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VARIATION OF SEED PRODUCTION AND VIABILITY IN A FULL-SIB TRIAL OF Melaleuca cajuputi sub sp. cajuputi IN GUNUNGKIDUL YOGYAKARTA Baskorowati, Liliana; Susanto, Mudji; Prasetyono, Prasetyono; Kartikawati, Noor; Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 9, No 2 (2012): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

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Abstract

Family variation of capsule production and the seed viability in the Paliyan full-sib trial of Melaleuca cajuputi subsp. cajuputi, at Gunungkidul, Yogyakarta, were observed. The full-sib trial was designed as Incomplete Block Design, consisting of 39 families; six individual as tree plot and replicated in eight blocks. Height and diameter at breast height were assessed to identify the correlation between capsule category and growth performance. The capsule production was assessed visually to one of the capsule categories: “0” for none; “1” for light; “2” for medium and “3” for heavy. Results showed that each family of M. cajuputi sub sp. cajuputi equally contributed  to the seed production.   Progeny analysis showed that the seed productions were not strongly under genetic control (h2i) = 0.12. This study also found positive correlation between the flowering strength (the flower production levels) and the number of capsule (R2=0.279). However, there were no significant differences between the flowering levels and the viability of M. cajuputi sub sp. cajuputi. Mean seed viability was 31%,  there were no significantly differences of capsules production between trees having high flowering intensity and low flowering intensity. Low seed viability was assumed due to the unsynchronicity of flowering, leading to the low levels of outcrossing rate. Therefore, selection of families with synchronicity of flowering was recommended to establish a seed orchard.
Improvement of Seed Orchard Management Based on Mating System of Cajuputi Trees Kartikawati, Noor Khomsah; Naiem, Mohammad; Hardiyanto, Eko Bhakti; Rimbawanto, Anto
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Breeding plan of cajuputi in Indonesia is aimed to increase plantation productivity of oil yield and 1.8 cineole content. Seed orchard of cajuputi at Paliyan, Gunungkidul, established using selected and genetically improved materials, has been producing seeds for operational plantation. This seed orchard would perform optimally if the mating systems of all individuals contribute to the inheritance of all genetic potential of the offsprings. Therefore, investigation of the mating systems of cajuputi was indispensible. The study has been carried out on 10 selected mother trees and the 24 offsprings of each mother trees using 8 microsatellite markers of nuclear DNA, namely Hin-2 (100-132 bp), Hin-4 (79-114 bp), Hin-5 (128-148 bp), Hin-7 (136-224 bp), Sal-1 (93-99 bp), Sal-3 (118-219 bp), Xho-1 (96-111 bp) and Xho-4 (150-216 bp), respectively. The result showed relatively high genetic variation of the offspring (HE=0.602, HO=0.594) originated from parent trees in the seed orchard. Parent trees tend to outcross(tm=0.951, ts=0.806), although seeds originated from biparental inbred (tm – ts = 0.145) and correlated paternity(rp=0.098) have also been observed. This genetically viable population could maintain its reproduction fi tness forshort term and adapt to the dynamic environmental changes for long term.Key words: mating system, cajuputi, seed orchard, microsatellite
VARIATION OF SEED PRODUCTION AND VIABILITY IN A FULL-SIB TRIAL OF Melaleuca cajuputi sub sp. cajuputi IN GUNUNGKIDUL YOGYAKARTA Baskorowati, Liliana; Susanto, Mudji; Prasetyono, Prasetyono; Kartikawati, Noor; Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 9, No 2 (2012): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2012.9.2.73-80

Abstract

Family variation of capsule production and the seed viability in the Paliyan full-sib trial of Melaleuca cajuputi subsp. cajuputi, at Gunungkidul, Yogyakarta, were observed. The full-sib trial was designed as Incomplete Block Design, consisting of 39 families; six individual as tree plot and replicated in eight blocks. Height and diameter at breast height were assessed to identify the correlation between capsule category and growth performance. The capsule production was assessed visually to one of the capsule categories: “0” for none; “1” for light; “2” for medium and “3” for heavy. Results showed that each family of M. cajuputi sub sp. cajuputi equally contributed  to the seed production.   Progeny analysis showed that the seed productions were not strongly under genetic control (h2i) = 0.12. This study also found positive correlation between the flowering strength (the flower production levels) and the number of capsule (R2=0.279). However, there were no significant differences between the flowering levels and the viability of M. cajuputi sub sp. cajuputi. Mean seed viability was 31%,  there were no significantly differences of capsules production between trees having high flowering intensity and low flowering intensity. Low seed viability was assumed due to the unsynchronicity of flowering, leading to the low levels of outcrossing rate. Therefore, selection of families with synchronicity of flowering was recommended to establish a seed orchard.
GENETIC DIVERSITY OF Dendrocalamus asper IN JAVA REVEALED BY RAPD MARKERS Rimbawanto, Anto
Indonesian Journal of Forestry Research Vol 3, No 1 (2006): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2006.3.1.67-74

Abstract

Dendracalamusasper is  one of  the most widely use and widely distributed bamboo  species in Indonesia. A genetic conservation plot has been established to maintain genetic diversity of the species. A total of  115 samples  were collected from 12 sites throughout Java. Genetic diversity was revealed using 31   RAPD  primers  producing  64 polymorphic  bands.  A total  of  81  different  multi-locus genotypes (genets) were identified between 115 samples. TI1e total number of multi-locus genotypes in all populations ranged from 3 to 9 (mean 6.5),  indicating that all populations consisted of multiple genotypes. Mean genetic diversity was 0.04, (ranging from the lowest 0.009 in Kuningan population to the highest 0.144  in Purwokerto population). The Simpsons diversity index indicated  that the most population composed of  unique genotype. Geographical differences did not correlate with genetic cluster.