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Syafrida Manuwoto
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Gangguan fisiologi dan biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) akibat perlakuan ekstrak campuran Tephrosia vogelli dan Piper aduncum Eka Chandra Lina; Dadang Dadang; Syafrida Manuwoto; Gustini Syahbirin
Jurnal Entomologi Indonesia Vol 12 No 2 (2015): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.723 KB) | DOI: 10.5994/jei.12.2.100

Abstract

Plant extracts have been known to cause physiological and biochemical interferences against insect, such as feeding inhibitor, food assimilation, and changes on activity of cytochrome b5 and cytochrome P450. This study was carried out to examine the effect of the extracts mixture of Tephrosia vogeliiand : Piper aduncum (1 : 5) on the physiology of Crocidolomia pavonana (F.) as well as extract of P. aduncum on biochemical of C. pavonana. The study showed that larvae of C. pavonana was experiencing a feeding inhibition until 94.82% after treated with extracts mixture on concentration 0.06% or equivalent to LC95. However, larvae treated with extracts mixture on LC25 and LC50 only experienced a relative growth disorders as a result of intrinsic toxicity of extracts mixture which enters into the body of insects. Insect adaptation to toxic plant compounds indicated by an increase in the digestibility of larvae approximately 11.11%. Furthermore, detoxification mechanism by larvae against active compounds of P. aduncum occured and shown by an increase of oxidative enzyme activity of cytochrome b5 and cytochrome P450, in in vivo and in vitro compared to control. This study provides an information about mode of action of extracts mixture of T. vogelii and P. aduncum (1 : 5) on larvae of C. pavonana and detoxification mechanism by larvae due to extract of P. aduncum
Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae) N. Usyati; Damayanti Buchori; Syafrida Manuwoto; Purnama Hidayat; Inez H. Slamet -Loedin
Jurnal Entomologi Indonesia Vol 6 No 1 (2009): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.28 KB) | DOI: 10.5994/jei.6.1.30

Abstract

Transformation two cry genes (cryIB-cryIAa) and transformation with a single the cry1B gene under the control of a wound-inducible maize proteinase inhibitor gene (mpi) promoter were two approaches that were used to get resistant rice to the rice stemborer which may be had a durable resistance. To obtain information on the effectiveness of seven transgenic rice lines to the rice yellow stemborer S. incertulas, a test was conducted in greenhouse. The seven lines were 1). line 4.2.3 and 2) line 4.2.4 both contain fusion of two cry genes (cryIB-cryIAa); 3) line 3R9 and 4) line 3R7 lines both contain of mpi-cryIB gene; and 5) line 6.11 contains of cryIAb gene by particle bombardment, 6) line DT-cry (Azygous) that do not contain cry gene (null), and 7) DT-cry line contains cryIAb gene by Agrobacterium, and as a negative control, we used three non transgenic rice varieties i.e., Rojolele, Cilosari, and Ciherang. The result showed that transgenic rice lines, except DT-cry and DT-cry (Azygous) lines were effective to suppress damage by the insect, and showed an inhibition effect on the growth of S. incertulas, and had a high level ofresistance than non transgenic rice varieties had. There were differences on resistance value/level among transgenic rice lines. Based on the resistance value, 6.11 line was the highest followed by 4.2.4 line and 3R7 line, these lines were categorized as high resistance. Transgenic rice-4.2.3 line and 3R9 line were categorized as moderat resistance. DT-cry and DT-cry (Azygous) lines were susceptible.
Komunitas serangga pada tanaman orok-Orok (Crotalaria striata) di berbagai habitat Hasmiandy Hamid; Damayanti Buchori; Syafrida Manuwoto; Hermanu Triwidodo
Jurnal Entomologi Indonesia Vol 4 No 2 (2007): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1379.23 KB) | DOI: 10.5994/jei.4.2.86

Abstract

To study insect community at orok-orok plantation (Crotalaria striata) from different habitat, we observed insects that interacted with Crotalaria plantation. There were four habitat type that used in this research, habitat open area and others were agroforestry habitat. We counted about nine ordo from insect that interacted with Crotalaria plant. More than 70% member of ordo, include to Hymenoptera, specially Formicidae. Not all function member of the ordo that known, but some of them are pollinator, parasitoid, predator and herbivore.
Co-Authors DADANG DADANG DAMAYANTI BUCHORI EKA CHANDRA LINA Gustini Syahbirin Hasmiandy Hamid Hermanu Triwidodo Inez H. Slamet -Loedin N. Usyati PURNAMA HIDAYAT