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All Journal Majalah Ilmiah Peternakan Journal of the Indonesian Tropical Animal Agriculture
M. Riyadhi
Study Program of Animal Production, Faculty of Agriculture, Lambung Mangkurat University, Jl. A. Yani km 36. Banjarbaru, South Kalimantan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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SPERM MORPHOLOGICAL ASSESSMENTS OF FRIESIAN HOLSTEIN BULL SEMEN COLLECTED FROM THREE ARTIFICIAL INSEMINATION CENTERS IN INDONESIA Purwantara, B.; Arifiantini, R.I.; Riyadhi, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 2 (2010): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.2.90-94

Abstract

Morphologically abnormal sperm in semen has been associated with the sub fertility and sterilityfor many years. This study assessed the sperm morphology of Friesian Holstein bull semen which wascollected from three Artificial Insemination centers in Indonesia. Total of 22 bulls were used in thisstudy; an ejaculate from each bull was examined. Three to four glass slides were prepared for each bullsample; a drop of semen was placed on each glass slide, smeared, and air-dried. The smeared sampleswere stained with carbolfuchsin-eosin (Williams stain). Morphological abnormality types were recordedfrom total of 500 spermatozoa. Results demonstrated that all 22 bulls had low sperm abnormality(<10%). Pearshaped was the most frequently type of sperm abnormality found in the samples(0.81±0.93%); while detached head was the lowest (0.01±0.04%).
PENGARUH BERBAGAI KONSENTRASI LAKTOSA DAN SARI KEDELAI PADA KUALITAS SEMEN KAMBING BOER YANG DIPRESERVASI M.A., NOVIAN; NURSYAM, A. S.; RIYADHI, M.; RIZAL, M.
Majalah Ilmiah Peternakan Vol 27 No 1 (2024): Vol. 27 No. 1 (2024)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MIP.2024.V27.i01.p01

Abstract

This study aims to determine the effect of lactose and soybean milk concentrations as semen extenders for Boer goat semen. The research method employed a Completely Randomized Design (CRD), consisting of four treatments and six replications, namely: 80% lactose + 20% egg yolk as a control (P0), 97% lactose + 3% soybean milk (P1), 95% lactose + 5% soybean milk (P2), and 93% lactose + 7% soybean milk (P3). The semen samples were then stored at 3-5oC, and motility and live spermatozoa percentage were observed daily for four days. The results obtained from the research indicated that the concentration of soybean milk significantly affected (P<0.05) the motility and live spermatozoa percentage of Boer goats after cooling on the third day. The combination of 3% soybean milk with 97% lactose as an extender resulted in improved motility and live spermatozoa percentage in Boer goat semen.
Co-Authors B. Purwantara M. Rizal M.A., NOVIAN NURSYAM, A. S. R.I. Arifiantini