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All Journal Indonesian Journal of Laboratory Inisiatif: Jurnal Ekonomi, Akuntansi Dan Manajemen
Istini Istini
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Mathematics Mechanical Engineering Medicine & Pharmacology Physics

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Pengaruh Penambahan Serum Dan Lama Waktu Inkubasi Lateks Terhadap Aktivitas Fagositosis Makrofag Tikus Sprague Dawley (SD) Dalam Menunjang Kegiatan Penelitian Istini Istini; Budi Santoso
Indonesian Journal of Laboratory Vol 1 No 1 Tahun 2018
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijl.v1i1.40965

Abstract

Fagositosis makrofag peritoneal dari hewan coba merupakan salah satu penelitian yang berkaitan dengan sistem imun tubuh. Fagositosis merupakan reaksi makrofag memfagosit/memakan lateks, tetapi reaksi tersebut kadang terkendala karena lateks tidak difagosit oleh makrofag. Perlu penambahan serum normal dari hewan coba bersangkutan, namun waktu inkubasi masih belum optimal. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serum pada lateks dan lama waktu inkubasi sehingga terjadi reaksi fagositosis yang optimal untuk selanjutnya dapat dipakai peneliti sebagai patokan dalam bekerja. Waktu inkubasi menggunakan 5 perlakuan : (1) makrofag + lateks tanpa serum inkubasi 60 menit, (2) makrofag + lateks + serum inkubasi 60 menit, (3) makrofag + lateks + serum inkubasi 45 menit, (4) makrofag + lateks + serum inkubasi 30 menit, (5) makrofag + lateks + serum inkubasi 15 menit. Hasil penelitian menunjukkan bahwa waktu penambahan lateks yang mengandung serum dan diinkubasi selama 60 menit dan 45 menit yaitu lateks yang difagosit oleh makrofag terlalu banyak, sedangkan untuk waktu inkubasi 30 menit dan 15 menit lateks yang difagosit makrofag tidak terlalu banyak. Kesimpulan: apabila penelitian fagositosis makrofag dari tikus Sprague Dawley(SD) dengan lateks mengalami kendala yaitu lateks tidak di fagosit oleh makrofag maka dapat ditambahkan serum pada lateks, sedangkan waktu inkubasi makrofag dengan lateks yang mengandung serum selama 15-30 menit
Pengaruh Kompetensi dan Loyalitas Terhadap Kinerja Karyawan PT Pegadaian CP Ponorogo Yang Dimediasi Oleh Kemampuan Kerja Ade Syahrizal; Agatha Tyagitha; Istini Istini; Ahmad Shofi Muzadi; Chamariyah Chamariyah
Inisiatif: Jurnal Ekonomi, Akuntansi dan Manajemen Vol. 4 No. 2 (2025): April : Inisiatif : Jurnal Ekonomi, Akuntansi dan Manajemen
Publisher : Universitas 45 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30640/inisiatif.v4i2.4142

Abstract

Ade Syahrizal, 2024. The Influence of Competence and Loyalty on the Empolyee Performance of PT Pegadaian CP Ponorogo Mediated by Work Ability. Wijaya Putra University Postgraduate Program Surabaya. This type of research is explanatory research, the approach used is a quantitative approach, the research sample is 46 people. The analysis tool uses Smart PLS (Partial Least Square). Research objectives: 1) To determine and analyze the influence of competence on the employee performance of PT Pegadaian CP Ponorogo. 2) To determine and analyze the influence of competence on the work ability of the PT Pegadaian CP Ponorogo. 3) To determine and analyze the influence of loyalty on the employee performance of PT Pegadaian CP Ponorogo. 4) To determine and analyze the indirect influence of loyalty on the work ability of PT Pegadaian CP Ponorogo. 5) To determine and analyze the indirect influence of work ability on the employee performance of PT Pegadaian CP Ponorogo. 6) To determine and analyze the indirect influence of competence on the employee performance of PT Pegadaian CP Ponorogo which is mediated by work ability. 7) To determine and analyze the indirect influence of loyalty on the employee performance of PT Pegadaian CP Ponorogo. The research results show that: 1) Competence has a positive and significant effect on employee performance as evidenced by the original sample value of 0.369 and p-values ​​of 0.000. 2) Competence has a significant positive effect on work ability as proven by the original sample value of 0.373 and p-values ​​of 0.002. 3) Loyalty has a positive and significant effect on employee performance as evidenced by the original sample value of 0.492 and p-values ​​of 0.000. 4) Loyalty has a significant positive effect on work ability as proven by the original sample value of 0.378 and p-values ​​of 0.000. 5) Work ability has a significant positive effect on employee performance as evidenced by the original sample value of 0.378 and p-values ​​of 0.000. 6) Competence has a positive and significant effect on employee performance which is mediated by work ability as evidenced by the original sample value of 0.141 and p-values ​​of 0.013. 7) Loyalty has a positive and significant effect on employee performance which is mediated by work ability as evidenced by the original sample value of 0.186 and p-values ​​of 0.001.
Co-Authors Ade Syahrizal Agatha Tyagitha Ahmad Shofi Muzadi Budi Santoso Chamariyah Chamariyah