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All Journal Journal of Dentomaxillofacial Science
Gostry A. Dohude
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
Dentistry

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The effectivity of binahong (anredera cordifolia (ten.) steenis) leaves extract for growth inhibition of streptococcus mutans in oral cavity Gostry A. Dohude; Frisca CM. Ginting
Journal of Dentomaxillofacial Science Vol. 6 No. 3 (2021): (Available online: 1 December 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i3.1040

Abstract

Objective: This research was conducted to determine the effectiveness of binahong (anredera cordifolia (ten.) steenis) leaves extract in inhibiting the streptococcus mutans growth.Material and Methods:  The study is true experimental laboratories with posttest-only control group design. The study sample used was binahong leaves and streptococus mutans bacteria that were multiplied in Microbiology Laboratory of the Faculty of Pharmacy, Universitas Sumatera Utara. This study used seven treatment groups namely, 75%, 50%, 25%, 12.5%, 6.25% binahong leaves extract, amoxicillin (positive control) and Dimethyl Sulfoxide (DMSO) (negative control). Testing the treatment group for the streptococcus mutans was carried out using the Kirby-Bauer disk diffusion method with four repetitions. Extract was done by maceration using 96% ethanol solvent.Results: Indicate that the binahong leaves extract can inhibit the growth of streptococcus mutans which can be seen from the clear zone formed on paper discs that have been given binahong leaves extract with a concentration of 75%, 50%, 25%, 12.5%, 6.25%, along with amoxicillin and dimethylsulfoxide/DMSO. The results also showed a significant difference in diameter of the inhibition zone from each treatment group (p <0.005).Conclusion: Binahong leaves extract has antibacterial ability because it can inhibit the growth of Streptococcus mutans in the oral cavity and compared to other concentrations, 75% concentration is more effective in inhibiting the growth of streptococcus mutans in the oral cavity.
Inhibition test of the pirdot leaf (saurauia vulcani korth) extract to the growth of streptococcus mutans bacteria in oral cavity with in vitro Gostry A. Dohude; Noviyanti
Journal of Dentomaxillofacial Science Article In Press
Publisher : DiscoverSys Inc.

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Abstract

Objective: This study aims to determine effectiveness of pirdot leaf (Saurauia vulcani Korth) extract as an antibacterial against the growth of Streptococcus mutans bacteria. Material and Methods: The study is experimental laboratories with post-test only control group design. The study sample use Streptococcus mutans bacteria that multiply and cultivation in Microbiology Laboratory of the Pharmacy Faculty, Universitas Sumatera Utara. Pirdot leaves that used for this research is collected from Sipangan Bolon village, Girsang regency, North Sumatera, Indonesia and extracted at the Laboratory of Traditional Medicine at the Faculty Pharmacy in Universitas Sumatera Utara. This research use six treatment group concentration of 50%, 25%, 12,5%, 6,25% from pirdot leaves extract, Amoxicillin (positive control), and Aquadest (negative control). The testing in six treatment group for the Streptococcus mutans bacteria are using diffusion method namely Kirby-Bauer with four repetition. The production to get extraction of pirdot leaf used maceration method and using 70% ethanol as a solvent. Results : This research indicate that Pirdot (Saurauia vulcani Korth) leaf can inhibit growth of Streptococcus mutans bacteria that can be seen from clear zone at paper discs that have been given Pirdot leaves extract with 50%, 25%, 12,5%, 6,25% concentration, Amoxicillin (positive control), and Aquadest (negative control). The results also showed a significant difference from diameter of the inhibition zone at each treatment group (p<0,005). Conclusion: Pirdot leaf extract has antibacterial effect that can inhibit the growth of Streptococcus mutans in oral cavity with in vitro. At a concentration of 50% is more effective to inhibit the growth of Streptococcus mutans in oral cavity with in vitro.
Inhibition test of the local anesthetics articaine 4% with adrenaline 1:100000 to the growth of staphylococcus aureus bacteria in vitro Gostry A. Dohude; Wahyu Ardi
Journal of Dentomaxillofacial Science Article In Press
Publisher : DiscoverSys Inc.

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Abstract

Objective: This research was conducted to determine the effectiveness of 4% local anesthetic with 1:100000 adrenaline in inhibiting the Stahapylococcus aureus growth. Material dan Methods : The study is true experimental laboratories with posttest-only control group design. This study sample was Articain 4% with 1:100000 adrenaline and Staphylococcus aureus bacteria that were multiplied in Microbiology Laboratory of the Faculty of Pharmacy, University of North Sumatera. The Articain 4% used in this research were obtained from one of dental suppliers in Jakarta. This study used three treatment groups namely Articaine 4%, Amoxicillin (positive control), and Aquades (negative control). Testing the treatment group for the Staphylococcus aureus was carried out using the Kirby-Bauer disk diffusion method with nine repetitions. Results : The result from this research indicate that Articaine 4% with 1:100000 adrenaline can inhibit the growth ofStaphylococcus aureus that can be seen from clear zone at paper discs that have been given Articaine 4%, Amoxicillin (positive control), and Aquadest (negative control). The results also showed a significant difference from diameter of the inhibition zone at each treatment group (p<0,005). Conclusion: Articaine 4% with 1:100000 adrenaline has antibacterial effect that can inhibit the growth of Staphylococcus aureus in oral cavity with in vitro.
Co-Authors Frisca CM. Ginting Noviyanti Wahyu Ardi