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THE EFFECT OF TEMPERATURE AND STORAGE OF COW'S MILK WATER ON LACTIC ACID LEVELS Yuyun Nailufar
Jurnal Kesehatan Tambusai Vol. 3 No. 1 (2022): Maret 2022
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v3i1.3529

Abstract

Introduction: Fresh milk is a food that is very high in nutritional value. Cow's milk contains lactose and organic salts secreted by the mammary glands and contains bifidus factor which functions for the growth of Lactobacillus bifidus bacteria which can inhibit pathogenic bacteria. The more lactic acid bacteria in cow's milk, the lower the pH of cow's milk because it produces lactic acid. So that the growth of destructive bacteria and pathogens will be inhibited, so it is possible that an increase in the number of bacteria can provide good durability in cow's milk. Method: This quantitative study aims to determine the effect of temperature and storage time on lactic acid levels in cow's milk. The subject of this research is the milk of dairy cows in the Yogyakarta City farm. The sampling technique that will be used is purposive sampling. The instrument used to measure the levels of lactic acid in cow's milk is a set of titration tools. Analysis of the data used is the calculation of lactic acid levels using the acid-base titration formula. Results: The results of the calculation of the entire sample range from 0.1800 to 0.1890, so that a maximum reference value of 0.2% is obtained. The calculation results for all samples range from 0.1710 to 0.1962, so that a maximum reference value of 0.2% is good. This value can be said that the length of storage of cow's milk for 5 hours at a refrigerator temperature still has a good value for the number of lactic acid bacteria. Conclusion: The effect of temperature before storage on lactic acid levels in cow's milk still has good quantity and quality. The effect of storage time for cow's milk within 5 hours also still has the value and quality of lactic acid levels.
The description of syphilis examination results using the immunochromatography method based on gender and age Yuyun Nailufar; Sri Ramadhani E Akas; Anis Putri Fauziah; Novitawati Novitawati; Margareta Fety Oktavia
JHeS (Journal of Health Studies) Vol. 9 No. 2 (2025): September
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/jhes.4392

Abstract

Syphilis is a sexually transmitted infection (STI) caused by the bacterium Treponema pallidum. Syphilis screening can be performed using the immunochromatography method. Puskesmas (Primary Health Center) Gondokusuman II, Yogyakarta Municipality, provides syphilis screening facilities using the immunochromatography method. This study aims to describe the results of syphilis screening using the immunochromatography method. This study employed descriptive quantitative. The sample of this study was the results of syphilis screening from January 2024 to June 2025 using the Slovin formula, and obtained results from 81 respondents. Based on this study, the results of syphilis screening were predominantly male aged 20-25 years (64.2%), compared to females (35.8%). A high percentage was found in men of productive age or adulthood. This age group has a high curiosity, so it tends to encourage trying new things. The high reactive results based on the results of the examination indicate the potential for infection to spread in the population. The conclusion shows that syphilis examinations in January 2024-June 2025 using the immunochromatography method were dominated by male gender aged 20-25 years or productive age and adult age.
Effectiveness of red spinach (Amaranthus tricolor) extract as an alternative to eosin in histological staining Yuyun Nailufar; Aji Bagus Widyantara; Yastifani Ilisne Kaka Mesa; Dritantiara Suci Manikum
JHeS (Journal of Health Studies) Vol. 10 No. 2 (2026): September
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/jhes.4949

Abstract

The high usage of eosin as a cytoplasmic stain in histological preparations involves relatively high costs and poses potential environmental and toxicity risks, thereby necessitating a natural dye alternative. Red spinach (Amaranthus tricolor) contains anthocyanins, which have the potential to be used as a substitute for eosin. This study aim to compare the effectiveness of red spinach extract with eosin as a cytoplasmic stain in histological preparations. This study employed a quasi-experimental laboratory design with a descriptive quantitative approach involving 24 myoma tissue preparations divided into four groups: Hematoxylin-Eosin (control), and Hematoxylin combined with red spinach extract at concentrations of 10%, 20%, and 40%. Staining quality was assessed based on cytoplasmic staining intensity, nucleus-cytoplasm contrast, and tissue structure clarity by an anatomical pathologist. Statistical analysis was performed using the Kruskal-Wallis test followed by the Mann-Whitney test. The results showed a significant difference between the control group and all red spinach extract groups (p < 0.001). The Hematoxylin-Eosin group obtained the highest mean rank (21.50), while all red spinach extract groups shared the same mean rank (9.50). The findings indicate that red spinach extract at all tested concentrations has not been able to provide a staining quality equivalent to that of eosin. Unlike red spinach extract, which is a mixture of various natural compounds, eosin possesses more standardized chemical characteristics and usage, resulting in a more consistent dye. Staining histological preparations using hematoxylin combined with red spinach extract at concentrations of 10%, 20%, and 40% has not been able to produce a quality equivalent to eosin. This applies to the conditions of this study using extract concentrations of 10%, 20%, and 40% via the maceration method; therefore, it cannot yet be concluded that red spinach extract is generally unsuitable as an alternative to eosin. Future research is recommended to use higher extract concentrations, more optimal extraction methods, and longer staining durations.