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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Heart Science Journal

Muhammad Saifur Rohman

Laboratorium Biokimia Fakultas Kedokteran Universitas Brawijaya

Author-ID : 363330

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

Published : 4 Documents Claim Missing Document

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Articles

Konstruksi Open Reding Frames (ORF) Artifisial Berukuran 798-bp yang Menyandi Protein dengan Urutan Asam Amino Acak Prijambada, Irfan Dwidya; Al-Awally, Khotibul Umam; Rohman, Muhammad Saifur; Artama, Wayan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2004): February 2004
Publisher : Universitas Atma Jaya Yogyakarta

Penyusunan pustaka dari open reading frames (ORF) buatan yang tersusun atas 798 bp (pasangan basa), 576Â di antaranya tersusun secara acak, yang mampu menyandi 266 asam amino telah berhasil dilakukan. Dalam upaya penyusunan tersebut diperoleh 32 transforman,Â lima di antaranya membawa ORF buatan. Dari kelima transforman yang membawa ORF buatan tersebut, hanya satu transforman yang mampu berekspresi dan menyandi suatu protein. Protein yang dihasilkan memiliki ukuran 17 kDa, berukuran lebih kecil daripada ukuran yang diharapkan yaitu 29 kDa.

Interaction Of Platelet Activating Factor Acetyl Hydrolase (Paf Ah) Enzyme In Gln281 To Arg281 Mutation Toward Paf And Its Molecular Dynamic Putri, Jayarani Fatimah; ., widodo; Rohman, Muhammad Saifur
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Platelet Activating Factor Acetyl Hydrolase (PAF AH) or LpPLA2 is key enzyme in myocardial infarction catalyzes the sn-2 acetyl group of Platelet Activating Factor (PAF) into lyso PAF and acetate as non-potent inflammatory molecules. PAF AH plays a critical role in arterial plaque development of Coronary Artery Disease (CAD). A crystal structure of PAF AH complexes with other ligand and effects of amino acid alteration to protein plasma consequence have also been reported. Here we report on the result of molecular docking and Molecular dynamic (MD) simulation carried out for PAF AH wild type (WT)/PAF and mutant Q281R/PAF complexes. Docking result shown that amino acid residues on active site of Q281 PAF AH mutant have not recognized on PAF AH. Eelectrostatics and hydrophobic bonds significantly reduced in Q281R than wild type. In the 7500 ps MD simulation Q281R showed less dynamics than WT but enzymatic machinary of mutant Q281R was not interrupted during MD simulation as well as PAF AH wildtype. These findings clearly indicated the importance effect of mutant Q281R in PAF AH recognition to its substrate

Hibiscus Sabdariffa Linn) terhadap NF-ĸβ, TNF-α dan ICAM-1 pada Human Umbilical Vein Endothelial Cells (HUVECs) Cultured yang dipapar Low Density Lipoprotein (LDL) Teroksidasi Sarbini, Dwi; Sargowo, Djanggan; Rohman, Muhammad Saifur
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Graduate School, Universitas Brawijaya

Tujuan penelitian ini adalah mengetahui efek dan mekanisme kerja ekstrak teh Rosella merah (Hibiscus sabdariffa Linn) terhadap aktifasi NF-ĸβ dan ekspresi protein TNF - α serta ICAM-1 yang menjadi mediator inflamasi pada aterosklerosis. Penelitian ini menggunakan kultur sel endotel yang diisolasi dari vena umbilikalis manusia ( HUVECs). Kelompok kontrol digunakan HUVECs tanpa paparan ox-LDL (kontrol negatif) dan HUVECs yang dipapar 40 µgml-1 Ox-LDL (kontrol positif). Kelompok perlakuan adalah HUVECs yang dipapar dengan berbagai dosis teh Rosella merah (0,01 mgml-1, 0,005 mgml-1 dan 0,001 mgml-1) dan diberikan selama 2 jam sebelum dipapar ox-LDL. Pengukuran aktifasi NF-ĸβ dilakukan setelah 30 menit paparan Ox-LDL menggunakan imunohistokimia. Ekspresi protein TNF-α dan ICAM-1 diukur setelah 24 jam dipapar Ox-LDL menggunakan imunohistokimia. Berdasarkan analisis ANOVA (p<0.01) terdapat efek penghambatan ekstrak teh Rosella merah (Hibiscus sabdariffa Linn) terhadap aktifasi NF-ĸβ dan ekspresi protein TNF -α serta ICAM-1 yang manjadi mediator terjadinya inflamasi pada aterosklerosis melalui penghambatan aktifasi NF-ĸβ. Terdapat hubungan negatif antara aktifasi NF-ĸβ dan ekspresi protein TNF -α serta ICAM-1 dengan dosis ekstrak teh Rosella merah (Analisis Spearman's [p<0,01, Correlation Coeff = -1]). Kata Kunci: atherosklerosis, ICAM-1, NF-ĸβ, Ox-LDL, Rosella merah (Hibiscus sabdariffa Linn), TNF-α

When bones meet blood vessels: BMP-2 expression and vascular calcification in a rat model of metabolic syndrome Sihotang, Fransiska Anggreni; Rohman, Muhammad Saifur; Satrijo, Budi; Sargowo, Djanggan; Rizal, Ardian
Heart Science Journal Vol. 7 No. 2 (2026): The Evolving Landscape of Heart Failure
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2026.007.02.14

Background: Vascular calcification (VC) is a significant contributor to cardiovascular morbidity, particularly in conditions like metabolic syndrome (MetS). Bone Morphogenetic Protein-2 (BMP-2) is implicated in the osteogenic differentiation of vascular cells, potentially linking MetS to VC. Objective: This study aimed to investigate aortic BMP-2 expression and the presence of VC in a rat model of MetS and assess the effects of Metformin, Empagliflozin, and a green tea/green coffee extract combination. Methods: Male Sprague-Dawley rats were induced with MetS using a high-fat, high-sucrose diet combined with a low-dose streptozotocin injection (30 mg/kgBW). Rats were divided into five groups (n=5): Normal control (NORM), MetS (METS), MetS + Metformin (MFN, 500 mg/kgBW), MetS + Empagliflozin (EMP, 30 mg/kgBW), and MetS + GTCE (300 mg/kgBW green tea + 200 mg/kgBW green coffee). Treatments were administered daily via oral gavage for 9 weeks. Result: Aortic tissue was collected for histological analysis and qRT-PCR to measure relative BMP-2 mRNA expression. Histological analysis revealed calcification in the aortic wall of the METS group rats. Compared to the NORM group, BMP-2 mRNA expression was significantly upregulated in the METS group (p<0.001). Treatment with MFN, EMP, and GTCE significantly downregulated BMP-2 mRNA expression compared to the METS group (p<0.001 for all). Conclusion: This study demonstrates that MetS induction in this rat model might promotes aortic calcification and significantly increases BMP-2 mRNA expression. Pharmacological interventions with Metformin, Empagliflozin, and green tea/coffee extract attenuated the MetS-induced upregulation of BMP-2 expression. These findings suggest a potential role for BMP-2 in MetS-associated vascular changes.

Co-Authors Al-Awally, Khotibul Umam Artama, Wayan Djanggan Sargowo Dwi Sarbini Fransiska Anggreni Sihotang Irfan Dwidya Prijambada Jamhari Jamhari Jayarani Fatimah Putri, Jayarani Fatimah Rizal, Ardian Satrijo, Budi

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