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Interaction Of Platelet Activating Factor Acetyl Hydrolase (Paf Ah) Enzyme In Gln281 To Arg281 Mutation Toward Paf And Its Molecular Dynamic Putri, Jayarani Fatimah; ., widodo; Rohman, Muhammad Saifur
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Platelet Activating Factor Acetyl Hydrolase (PAF AH) or LpPLA2 is key enzyme in myocardial infarction catalyzes the sn-2 acetyl group of Platelet Activating Factor (PAF) into lyso PAF and acetate as non-potent inflammatory molecules. PAF AH plays a critical role in arterial plaque development of Coronary Artery Disease (CAD). A crystal structure of PAF AH complexes with other ligand and effects of amino acid alteration to protein plasma consequence have also been reported. Here we report on the result of molecular docking and Molecular dynamic (MD) simulation carried out for PAF AH wild type (WT)/PAF and mutant Q281R/PAF complexes. Docking result shown that amino acid residues on active site of Q281 PAF AH mutant have not recognized on PAF AH. Eelectrostatics and hydrophobic bonds significantly reduced in Q281R than wild type. In the 7500 ps MD simulation Q281R showed less dynamics than WT but enzymatic machinary of mutant Q281R was not interrupted during MD simulation as well as PAF AH wildtype. These findings clearly indicated the importance effect of mutant Q281R in PAF AH recognition to its substrate
Action Plan in Developing Sea Turtle Conservation as Ecotourism Attraction in Sukamade, Meru Betiri National Park Haryati, Jehan Ramdani; Putri, Jayarani Fatimah; Chairiyah, Nurul; Harris, Ali; Putri, Helen Aulia; Pamungkas, Ratih Nila
Journal of Indonesian Tourism and Development Studies Vol. 4 No. 2 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2016.004.02.04

Abstract

Sukamade Coastal Area located in Meru Betiri National Park has particular object as ecotourism attraction, i.e. Sea Turtle. On the other hand, national park management use the cross subsidy from ecotourism activities to promote the conservation action plans for the survival of sea turtle. This study was aimed to formulate action plans to develop the sea turtle conservation as ecotourism attraction in Sukamade Coastal Area. Field observation was conducted to evaluate the current activities of the sea turtle conservation and ecotourism activities in Sukamade. Semi-structured interview and secondary data collection was also conducted to support the formulation of the action plan. Data was analyzed by SWOT method to be developed into action plan. Results showed that the management of sea turtle conservation and ecotourism activities in Sukamade Coastal Area has been attempted to meet the conservation concept for sea turtle but also beneficial for the ecotourism implementation. However, we recommend a long term and short term action plan for the sustainability of the sea turtle conservation and ecotourism activities in Sukamade Coastal area. It includes the mangrove restoration, sea turtle feed development, mapping of sea turtle distribution and migration, the use of up to date biotechnology, workshop on conservation management, and the development of research facility in the area. Additional to the current policy on sea turtle conservation and ecotourism activities, the action plan that we recommended were expected to improve the sustainability management in Sukamade Coastal Area. Keywords: Action Plan, conservation, ecotourism, Sea Turtle.