Vu Van Do
Institute of Tropical Biology, 01 Mac Dinh Chi str., dist. 1, Ho Chi Minh City

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SYNTHESIS OF FERRITE NANOPARTICLES WITH PROTEIN MOLECULES IMMOBILLIZED ON THEIR SURFACES Tran Hoang Hai; Le Hong Phuc; Doan Thi Kim Dung; Nguyen Thi Le Huyen; Bui Duc Long; Nguyen Tien Thang; Vu Van Do; Nguyen Thi Nhu Quynh; Masanori Abe
ASEAN Journal on Science and Technology for Development Vol. 25 No. 1 (2008): ASEAN Journal on Science and Technology for Development (AJSTD)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.061 KB) | DOI: 10.29037/ajstd.227

Abstract

The ferrite (a Fe3O4 – gFe2O3 mixed solution) fine particles, ~8 nm in size were synthesized from an aqueous solution. Trypsin, a proteolytic enzyme or a protein, was immobilized onto the surfaces of those particles during the synthesis process. The process was performed in the open air at a temperature as low as 4°C and on near-neutral condition of pH = 9, which is compatible with most of the bioactive molecules as well as trypsin. Therefore this technique is advantageous for preparing magnetite particles having biomolecules immobilized on their surfaces, which will be used for biomedical applications utilizing magnetic separation technique.