SYNTHESIS OF FERRITE NANOPARTICLES WITH PROTEIN MOLECULES IMMOBILLIZED ON THEIR SURFACES

Tran Hoang Hai (Institue of Physics in Ho Chi Minh City, 01 Mac Dinh Chi Str., Dist. 1, Ho Chi Minh City)
Le Hong Phuc (Institue of Physics in Ho Chi Minh City, 01 Mac Dinh Chi Str., Dist. 1, Ho Chi Minh City)
Doan Thi Kim Dung (Institue of Physics in Ho Chi Minh City, 01 Mac Dinh Chi Str., Dist. 1, Ho Chi Minh City)
Nguyen Thi Le Huyen (Institue of Physics in Ho Chi Minh City, 01 Mac Dinh Chi Str., Dist. 1, Ho Chi Minh City)
Bui Duc Long (Institue of Physics in Ho Chi Minh City, 01 Mac Dinh Chi Str., Dist. 1, Ho Chi Minh City)
Nguyen Tien Thang (Institute of Tropical Biology, 01 Mac Dinh Chi str., dist. 1, Ho Chi Minh City)
Vu Van Do (Institute of Tropical Biology, 01 Mac Dinh Chi str., dist. 1, Ho Chi Minh City)
Nguyen Thi Nhu Quynh (Institute of Tropical Biology, 01 Mac Dinh Chi str., dist. 1, Ho Chi Minh City)
Masanori Abe (Tokyo Institue of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552)

ASEAN Journal on Science and Technology for Development
Vol. 25 No. 1 (2008): ASEAN Journal on Science and Technology for Development (AJSTD)

The ferrite (a Fe3O4 – gFe2O3 mixed solution) fine particles, ~8 nm in size were synthesized from an aqueous solution. Trypsin, a proteolytic enzyme or a protein, was immobilized onto the surfaces of those particles during the synthesis process. The process was performed in the open air at a temperature as low as 4°C and on near-neutral condition of pH = 9, which is compatible with most of the bioactive molecules as well as trypsin. Therefore this technique is advantageous for preparing magnetite particles having biomolecules immobilized on their surfaces, which will be used for biomedical applications utilizing magnetic separation technique.