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Hamadah Mohd Nur Lubis
Faculty Science, University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Mathematics

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Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology Abd-El Aziem Farouk; Anis Shobirin Meor Hussin; Ralf Greiner; Shareef Mohideen Ismail; Hamadah Mohd Nur Lubis
ASEAN Journal on Science and Technology for Development Vol. 26 No. 1 (2009): ASEAN Journal on Science and Technology for Development (AJSTD)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.931 KB) | DOI: 10.29037/ajstd.304

Abstract

The production of phytase in a recombinant E.coli using the pBAD expression  system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3  central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature.  Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.   
Co-Authors Abd-El Aziem Farouk Anis Shobirin Meor Hussin Ralf Greiner Shareef Mohideen Ismail