Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

Abd-El Aziem Farouk (Faculty Science, University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia)
Anis Shobirin Meor Hussin (Faculty Science, University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia)
Ralf Greiner (Max Rubner-Institute, Department of Food and Bio Process Engineering, Haid-und-Neu-Straße 5, 76131 Karlsruhe)
Shareef Mohideen Ismail (Faculty Science, University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia)
Hamadah Mohd Nur Lubis (Faculty Science, University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia)

ASEAN Journal on Science and Technology for Development
Vol. 26 No. 1 (2009): ASEAN Journal on Science and Technology for Development (AJSTD)

The production of phytase in a recombinant E.coli using the pBAD expression  system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3  central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature.  Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.