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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Mikologi Indonesia NICHE Journal of Tropical Biology
Wahyu Aji Mahardhika
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Isolasi kapang endofit dari tanaman ciplukan (Physalis angulata L.) dan potensi antibakteri terhadap Escherichia coli dan Staphylococcus aureus Wahyu Aji Mahardhika; MG Isworo Rukmi; Sri Pujiyanto
NICHE Journal of Tropical Biology Vol. 4, No. 1, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.1.33-39

Abstract

Berbagai Kapang Aspergillus seksi Nigri dari Buah Kedondong Wahyu Aji Mahardhika; Isworo Rukmi
Jurnal Mikologi Indonesia Vol 6, No 1 (2022): Juni 2022
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v6i1.175

Abstract

Kapang merupakan cendawan berfilamen yang hidup pada berbagai subsrat dan memiliki habitat yang beragam, antara lain hidup pada tanah, air, serealia, endofit dalam tanaman, dan buah. Habitat kapang yang beragam juga memperbanyak diversitas kapang pada berbagai substrat, salah satunya pada buah kedondong. Salah satu kapang yang sering ditemukan adalah Aspergillus. Kapang Aspergillus terdiri dari beberapa grup salah satunya adalah Aspergillus seksi Nigri yang lebih dikenal dengan black mold. Tujuan dari penelitian ini adalah untuk mengidentifikasi kapang Aspergillus seksi Nigri pada buah kedondong yang telah rusak atau busuk. Isolasi kapang tersebut menggunakan metode secara langsung (purposive sampling) dengan mengambil kapang Aspergillus yang diduga berasal dari seksi Nigri, lalu diinokulasikan ke media PDA, MEA, dan CDA. Kapang kemudian diamati morfologinya secara makroskopis dan mikroskopis serta selanjutnya diidentifikasi untuk mengetahui spesiesnya. Pada penelitian ini diperoleh hasil sebanyak sembilan isolat yang berasal dari buah kedondong, diantaranya Aspergillus niger, Aspergillus foetidus, Aspergillus aculeatus, Aspergillus japonicus dan Aspergillus sp. Penelitian selanjutnya isolat tersebut dapat diidentifikasi secara molekuler dan diuji potensinya.
NEW RECORD OF Mycena chlorophos (BIOLUMINESCENT FUNGI) FOR INDONESIA Fathansah Nugeraha; Silva Ristiana Haryadi; Wahyu Aji Mahardhika; Oktan Dwi Nurhayat; Permana Putra, Ivan
BIOTROPIA Vol. 32 No. 3 (2025): BIOTROPIA Vol. 32 No. 3 December 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.3.2547

Abstract

HIGHLIGHTS- Glowing mushrooms were found at night in the forest of IPB University and showed a bright green light in the dark.- Examination of their morphological features and phylogeny material confirmed they are the species Mycena chlorophos.- This is the first confirmed record of Mycena chlorophos in Indonesia, extending its known distribution worldwide. ABSTRACTA bioluminescent fungus produce light as a result of a chemical reaction within its body. The majority of fungi exhibiting bioluminescence are classified within the Mycenaceae which comprises a significant proportion of the known glowing fungi. One of those species which well noted worldwide is Mycena chlorophos. However, no clear record of M. chlorophos has been discovered in Indonesia. While engaging in our routine late-night mushroom foraging in IPB University Campus Forest (IPBUCF), some glowing basidiomata were encountered. This study sought to determine the taxonomic classification of the collected specimens through comprehensive morphological and molecular examinations. The fresh fruiting bodies were underwent thorough examination based on macroscopic and microscopic characteristics. Phylogenetic analysis of the rDNA-ITS region was conducted using ITS 1/2 primers, and the integration of morphological and molecular data verified that our specimens belong to Mycena chlorophos. By morphology, our specimen poses convex pileus with shallow depression at centre, viscid, shiny, adnexed lamellae with series of lamellae, ellipsoid and smooth basidiospores. The pileus and hymenophore glow vividly with a bright greenish hue in the darkness. The BLAST analysis indicated a 98% similarity between our specimen and Mycena chlorophos from China, ranking it among the top matches. Furthermore, the phylogenetic tree positioned our specimens within the M. chlorophos clade with a 100% bootstrap support value. Our specimen was in sub clade with materials from Malaysia. The DNA sequence of our scpecimen has been registered to Gen Bank and can be used for future reference of bioluminescent fungi wordlwide. This research updates information regarding the distribution and characteristics of  M. chlorophos in Indonesia. The discovery of this species in the IPBUCF adds to the known geographical range of M. chlorophos near the anthropogenic activity in Indonesia. In addition, our result also contributes to the growing documentation of bioluminescent fungi in Indonesia, a region with high but underexplored fungal biodiversity.
Co-Authors Fathansah Nugeraha Ivan Permana Putra MG Isworo Rukmi Oktan Dwi Nurhayat Silva Ristiana Haryadi Sri Pujiyanto