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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Marine Research Omni-Akuatika Juvenil: Jurnal Ilmiah Kelautan dan Perikanan
Kartika Dewi
Program Studi Ilmu Kelautan, Fakultas Pertanian, Universitas Trunojoyo Madura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Concentration of Ammonia Pollution in Madura Traditional Salt Production Water Eka Nurrahema Ning Asih; Ary Giri Dwi Kartika; Makhfud Efendy; Kartika Dewi; Bahri Fadloli; Arya Galin Fakhrul Islami Zain
Journal Omni-Akuatika Vol 18 (2022): Omni-Akuatika Special Issue 4th Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2022.18.S1.980

Abstract

Ammonia (NH3) is one of the impurities in salt-producing water, which is often found in the traditional salt ponds of the Madurese. The purpose of this study was to determine the concentration of ammonia contamination as an impurity in salt production process water from raw water, reservoir ponds, evaporator ponds to geomembrane crystallizer ponds when salt production takes place in the salt ponds of Tanjungan Village, Bangkalan Regency. Determination of ammonia concentration using the phenate method based on SNI 06-6989.30-2005 with a spectrophotometer reading tool (UV-2700 Serial No. A11674900027) at a wavelength of 420 nm. The average concentration of ammonia as an impurity in raw materials in traditional pond ponds in Tajungan village, namely raw water is 2.31±0.24 mg/L, reservoir ponds are 2.56±0.57 mg/L, evaporator ponds are 2, 39±0.84 mg/L and the geomembrane crystallizer ponds are 1.37±0.09 mg/L. The highest average ammonia concentration was found in the reservoir ponds raw saltwater sample, while the lowest concentration was found in the geomembrane crystallizer ponds sample. The high average ammonia concentration in the salt ponds of Tajungan Village, Bangkalan Regency indicates that all raw saltwater in these ponds is contaminated with ammonia. The high concentration of ammonia in the salt ponds may be due to the entry of household waste. Keywords: (NH3), raw water, reservoir ponds, evaporation ponds, geomembrane crystallizer ponds
Karakterisasi Fisiologis Isolat bakteri Halofilik dari kolam Peminihan Tambak Garam Rakyat Di Kabupaten Pamekasan Kartika Dewi; Eka Nurrahema Ning Asih; Dewi Anugrah Fitri; Sri Astutik
Juvenil Vol 3, No 3 (2022)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v3i3.17074

Abstract

ABSTRAKKelompok bakteri slight halofilik dapat ditemukan di air laut pada kolam peminihan yang menjadi bahan baku utama dalam pembuatan garam di Pamekasan-Madura. Tujuan penelitian ini adalah untuk mengetahui karakteristik biokimia isolat bakteri halofilik yang di isolasi dari kolam peminihan pada tambak garam rakyat di Pamekasan-Madura. Metode yang digunakan dalam penelitian ini yaitu pengujian biokimia bakteri halofilik meliputi pewarnaan Gram, uji indol, katalase, dan oksidase, uji metil red, uji Voges Proskauer, asimilasi citrat, dan uji pemanfaatan sumber karbon. 3 isolat berhasil ditumbuhkan dalam media padat halophile menggunakan kadar garam sebesar 1% dengan kode P.P.1, P.P.2 dan P.P.3. Hasil uji indol ketiga isolat bakteri halofilik P.1, P.2 dan P3 menunjukkan hasil uji indol negatif.  Sumber karbon yang digunakan bakteri tersebut diantaranya glukosa, sukrosa dan mallosa dengan uji O/F, fermentative untuk isolat P.P.1 dan P.P.3 sedangkan non fermentatif untuk isolate P.P.2. Uji MR-VP menunjukkan hasil negatif untuk ke tiga isolat tersebut. Uji katalase dan uji oksidasi masing-masing isolat menunjukkan hasil positif, sehingga isolat P.P.1, P.P.2 dan P.P.3 termasuk bakteri aerob. Kata kunci: slight halofilik, kolam peminihan, uji biokimiaABSTRACTA diverse group of slight halophilic bacteria can be found in saline enviroment such purification water of traditional salt production Pamekasan-Madura. The existence of slight halophilic microbial communities from purification water of traditional salt production have not been used optimally. The aim of this study was biochemical characterization of the halophilic bacteria isolated from traditional salt production Pamekasan- Madura. The biochemical characterization were done using standart methodes which included indole test, catalase and oxidase activities, methyl red test, Voges-Proskauer (VP) test, citrate utilization test and carbohydrates utilisation . 3 isolates, P.P.1, P.P.2 and P.P.3 were successfully grown in halophile agar plates containing 2% of total salt. All of these isolates were negative for indole production and MR-VP test, but were positive for catalase and oxidase activities.  The carbon sources used by these isolates included glucose, sucrose and mallose with the O/F test was fermentative for P.P.1 and P.P.3 while non-fermentative for P.P.2. All of these isolates were identified as aerob bacteria.Keyword: slight halophilic, purification water, biochemical characterization
Bakteri halofilik dan halotoleran dari air baku tambak garam Universitas Trunojoyo Madura: Halophilic and halotolerant bacteria from raw water of salt ponds of Trunojoyo University Madura Salsabil Firda Nazhifan; Kartika Dewi; Eka Nurrahema Ning Asih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 1 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i1.44536

Abstract

Halophiles are bacteria that require specific salt concentrations to survive, while halotolerants are bacteria that grow in the absence of salt and in the presence of high salt concentrations. Both halophiles and halotolerant bacteria have potential biotechnological applications. The aim of this study was to isolate moderate halophiles and halotolerant bacteria from the raw water of salt ponds at Trunojoyo Madura University and to determine their morphological and biochemical characteristics. Four halophilic bacterial isolates with codes AB.1.2, AB.2.4, AB.2.5, and AB.3.6, and two isolates of halotolerant bacteria with codes AB.1.1 and AB.2.3, were obtained. All isolates were coccus shaped, and most of the isolates were gram-negative. Three isolates were identified as aerobic bacteria based on catalase and oxidase tests. Only halotolerant bacteria AB.1.1 and AB.2.3, showed positive results in the citrate utilization test, while halophiles AB1.2, AB.2.5, and AB.3.6, showed positive results in the urease test. All isolates, both halophiles and halotolerant, were able to utilize sucrose, glucose, mannitol, and maltose.
Deteksi Total Bakteri Escherichia coli Pada Sedimen Laut Perairan Desa Padelegan Sebagai Indikator Cemaran Mikrobiologis Wisata Pantai The Legend-Pamekasan Eka Nurhema Ning Asih; Annisa Ramadhanti; Ashari Wicaksono; Kartika Dewi; Sri Astutik
Journal of Marine Research Vol 13, No 1 (2024): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v13i1.37063

Abstract

Escherichia coli tergolong bakteri fecal coliform yang merupakan kelompok bakteri patogen sebagai indikator pencemaran mikrobiologi air. Tingginya jumlah bakteri tersebut di perairan Desa Padelegan diduga disebabkan oleh tekanan pencemaran dalam rumah tangga. Penelitian ini dilakukan untuk mengetahui kualitas bakteriologis khususnya bakteri Escherichia coli yang terakumulasi pada sedimen perairan Desa Padelegan terkait dengan keselamatan operasional kawasan wisata pantai The Legend di sekitar lokasi. Metode yang digunakan untuk analisis total bakteri Escherichia coli mengacu pada SNI 2332.1:2015. Hasil penelitian menunjukkan nilai total bakteri Escherichia coli pada 7 stasiun berkisar antara <3,0 MPN/g - 93 MPN/g. Total bakteri Escherichia coli pada seluruh stasiun tersebut berada di bawah ambang batas untuk keperluan wisata bahari sesuai Keputusan Menteri Negara Lingkungan Hidup No. 51 Tahun 2004 yaitu <200 MPN/g dengan toleransi <10%. Nilai analisis total bakteri Escherichia coli menunjukkan bahwa kualitas perairan khususnya sedimen di sekitar Desa Padelegan berada pada kategori masih baik dan aman untuk kegiatan wisata bahari. Escherichia coli is classified as Fecal coliform bacteria which is a group of pathogenic bacteria as an indicator of water microbiological contamination. The high total number of these bacteria in the waters of Padelegan Village is thought to have come from the pressure of domestic contamination. This study was conducted to determine the bacteriological quality, especially Escherichia coli bacteria that accumulate in the sediments of the waters of Padelegan Village related to the safety of operating beach tourism areas The Legend around the location. The method used for the total analysis of Escherichia coli bacteria refers to SNI 2332.1:2015. The results showed that the total value of Escherichia coli bacteria at 7 stations ranged from <3.0 MPN/g - 93 MPN/g. The total Escherichia coli bacteria at all of these stations were below the threshold for marine tourism purposes according to the Decree of the State Minister for the Environment No. 51 of 2004, namely <200 MPN/g with a tolerance of <10%. The value of the total analysis of Escherichia coli bacteria shows that the quality of the waters, especially the sediments around the village of Padelegan, is in the category that is still good and safe for marine tourism activities.
Co-Authors Annisa Ramadhanti Ary Giri Dwi Kartika Arya Galin Fakhrul Islami Zain Ashari Wicaksono Bahri Fadloli Dewi Anugrah Fitri Eka Nurhema Ning Asih Eka Nurrahema Ning Asih Makhfud Efendy Salsabil Firda Nazhifan Sri Astutik Sri Astutik