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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Marine Research Omni-Akuatika Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Zona Laut : Jurnal Inovasi Sains dan Teknologi Kelautan Jurnal Perikanan Jurnal Ilmu Kelautan Lesser Sunda
Eka Nurrahema Ning Asih
Universitas Trunojoyo Madura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Earth & Planetary Sciences Energy Engineering Environmental Science Social Sciences Transportation

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Concentration of Ammonia Pollution in Madura Traditional Salt Production Water Eka Nurrahema Ning Asih; Ary Giri Dwi Kartika; Makhfud Efendy; Kartika Dewi; Bahri Fadloli; Arya Galin Fakhrul Islami Zain
Journal Omni-Akuatika Vol 18 (2022): Omni-Akuatika Special Issue 4th Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2022.18.S1.980

Abstract

Ammonia (NH3) is one of the impurities in salt-producing water, which is often found in the traditional salt ponds of the Madurese. The purpose of this study was to determine the concentration of ammonia contamination as an impurity in salt production process water from raw water, reservoir ponds, evaporator ponds to geomembrane crystallizer ponds when salt production takes place in the salt ponds of Tanjungan Village, Bangkalan Regency. Determination of ammonia concentration using the phenate method based on SNI 06-6989.30-2005 with a spectrophotometer reading tool (UV-2700 Serial No. A11674900027) at a wavelength of 420 nm. The average concentration of ammonia as an impurity in raw materials in traditional pond ponds in Tajungan village, namely raw water is 2.31±0.24 mg/L, reservoir ponds are 2.56±0.57 mg/L, evaporator ponds are 2, 39±0.84 mg/L and the geomembrane crystallizer ponds are 1.37±0.09 mg/L. The highest average ammonia concentration was found in the reservoir ponds raw saltwater sample, while the lowest concentration was found in the geomembrane crystallizer ponds sample. The high average ammonia concentration in the salt ponds of Tajungan Village, Bangkalan Regency indicates that all raw saltwater in these ponds is contaminated with ammonia. The high concentration of ammonia in the salt ponds may be due to the entry of household waste. Keywords: (NH3), raw water, reservoir ponds, evaporation ponds, geomembrane crystallizer ponds
Karakterisasi Fisiologis Isolat bakteri Halofilik dari kolam Peminihan Tambak Garam Rakyat Di Kabupaten Pamekasan Kartika Dewi; Eka Nurrahema Ning Asih; Dewi Anugrah Fitri; Sri Astutik
Juvenil Vol 3, No 3 (2022)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v3i3.17074

Abstract

ABSTRAKKelompok bakteri slight halofilik dapat ditemukan di air laut pada kolam peminihan yang menjadi bahan baku utama dalam pembuatan garam di Pamekasan-Madura. Tujuan penelitian ini adalah untuk mengetahui karakteristik biokimia isolat bakteri halofilik yang di isolasi dari kolam peminihan pada tambak garam rakyat di Pamekasan-Madura. Metode yang digunakan dalam penelitian ini yaitu pengujian biokimia bakteri halofilik meliputi pewarnaan Gram, uji indol, katalase, dan oksidase, uji metil red, uji Voges Proskauer, asimilasi citrat, dan uji pemanfaatan sumber karbon. 3 isolat berhasil ditumbuhkan dalam media padat halophile menggunakan kadar garam sebesar 1% dengan kode P.P.1, P.P.2 dan P.P.3. Hasil uji indol ketiga isolat bakteri halofilik P.1, P.2 dan P3 menunjukkan hasil uji indol negatif.  Sumber karbon yang digunakan bakteri tersebut diantaranya glukosa, sukrosa dan mallosa dengan uji O/F, fermentative untuk isolat P.P.1 dan P.P.3 sedangkan non fermentatif untuk isolate P.P.2. Uji MR-VP menunjukkan hasil negatif untuk ke tiga isolat tersebut. Uji katalase dan uji oksidasi masing-masing isolat menunjukkan hasil positif, sehingga isolat P.P.1, P.P.2 dan P.P.3 termasuk bakteri aerob. Kata kunci: slight halofilik, kolam peminihan, uji biokimiaABSTRACTA diverse group of slight halophilic bacteria can be found in saline enviroment such purification water of traditional salt production Pamekasan-Madura. The existence of slight halophilic microbial communities from purification water of traditional salt production have not been used optimally. The aim of this study was biochemical characterization of the halophilic bacteria isolated from traditional salt production Pamekasan- Madura. The biochemical characterization were done using standart methodes which included indole test, catalase and oxidase activities, methyl red test, Voges-Proskauer (VP) test, citrate utilization test and carbohydrates utilisation . 3 isolates, P.P.1, P.P.2 and P.P.3 were successfully grown in halophile agar plates containing 2% of total salt. All of these isolates were negative for indole production and MR-VP test, but were positive for catalase and oxidase activities.  The carbon sources used by these isolates included glucose, sucrose and mallose with the O/F test was fermentative for P.P.1 and P.P.3 while non-fermentative for P.P.2. All of these isolates were identified as aerob bacteria.Keyword: slight halophilic, purification water, biochemical characterization
Bakteri halofilik dan halotoleran dari air baku tambak garam Universitas Trunojoyo Madura: Halophilic and halotolerant bacteria from raw water of salt ponds of Trunojoyo University Madura Salsabil Firda Nazhifan; Kartika Dewi; Eka Nurrahema Ning Asih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 1 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i1.44536

Abstract

Halophiles are bacteria that require specific salt concentrations to survive, while halotolerants are bacteria that grow in the absence of salt and in the presence of high salt concentrations. Both halophiles and halotolerant bacteria have potential biotechnological applications. The aim of this study was to isolate moderate halophiles and halotolerant bacteria from the raw water of salt ponds at Trunojoyo Madura University and to determine their morphological and biochemical characteristics. Four halophilic bacterial isolates with codes AB.1.2, AB.2.4, AB.2.5, and AB.3.6, and two isolates of halotolerant bacteria with codes AB.1.1 and AB.2.3, were obtained. All isolates were coccus shaped, and most of the isolates were gram-negative. Three isolates were identified as aerobic bacteria based on catalase and oxidase tests. Only halotolerant bacteria AB.1.1 and AB.2.3, showed positive results in the citrate utilization test, while halophiles AB1.2, AB.2.5, and AB.3.6, showed positive results in the urease test. All isolates, both halophiles and halotolerant, were able to utilize sucrose, glucose, mannitol, and maltose.
Penambahan ekstrak lamun (Enhalus acoroides) dan gonad bulu babi (Diadema setosum) sebagai formulasi sediaan moisturizer body lotion: Addition of seagrass (Enhalus acoroides) extract and sea urchin (Diadema setosum) gonads as formulation of moisturizer body lotion Lailatul Badriyah; Eka Nurrahema Ning Asih; Siti Nihayatun Ni’amah; Reza Hidayah Ningrum; Yuniar Mardiyanti; Destin Retno Wulansari
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 1 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i1.44880

Abstract

The skin is a part of the body that is sensitive to changes in temperature due to indoor and outdoor activities. A moisturizer body lotion made from seagrass and sea urchin gonads is a cosmetic product that can be used to treat dry and flaky skin. The purpose of this study was to determine the optimal formulation of moisturizer body lotion based on the characteristics of physical stability, the relationship between the irritation index and the panelist's preference test results, and the phytochemical content of the moisturizer body lotion made from seagrass (Enhalus acoroides) extract and sea urchin (Deadema setosum) gonads. This study consisted of extract preparation, body lotion preparation without the addition of seagrass extract and sea urchin gonads (control) and with addition (2:1, 1:2, and 3:3), body lotion physical stability test, irritation test, and phytochemical test. The results showed that there were color differences among the four body lotion formulations, namely white (control), celery (2:1), sage (1:2), and olive (3:3), with a distinct blossom odor. The pH range was 6.31-8.05 and its spreadability range was 3.2-6.7 cm. The results of the irritation index test from 32 panelists detected 3% indicating symptoms of irritation and severe edema (swelling), and 1% of the heat sensation index was detected. The highest panelist preference test values for color, aroma, and texture indicators were found in the product without the addition of seagrass extract and sea urchin gonads. Moisturizer body lotion preparations contain alkaloids and tannins. The best formulation is the ratio of seagrass extract to sea urchin gonads of 2:1, which is characterized by a high hedonic test (color), absence of irritation index edema, and feeling of heat.
Potensi Bakteri Halofilik Ekstrim dari Tambak Garam Tradisional sebagai Penghambat Aktivitas Bakteri Salmonella sp. Eka Nurrahema Ning Asih; Dewi Anugrah Fitri; Ary Giri Dwi Kartika; Sri Astutik; Makhfud Efendy
Journal of Marine Research Vol 12, No 3 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i3.35372

Abstract

Infeksi bakteri Salmonella sp. di perairan dapat merugikan perikanan budidaya, menurunkan kualitas hasil perikanan tangkap dan merusak kualitas produk olahan perikanan. Perlu dilakukan penelitian untuk mengatasi dan mencegah aktivitas bakteri Salmonella sp. pada beberapa sektor perikanan tersebut. Salah satu upaya yang bisa dilakukan adalah memanfaatkan sumberdaya bakteri halofilik yang hidup melimpah di air meja kristalisasi tambak garam tradisional sebagai agen penghambat aktivitas bakteri Salmonella sp.. Penelitian ini bertujuan untuk mengetahui potensi bakteri halofilik ekstrim yang dikultur dari tambak garam tradisional Pamekasan-Madura sebagai penghambat aktivitas bakteri Salmonella sp.. Metode yang digunakan dalam penelitian terdiri dari beberapa tahap yaitu isolasi, purifikasi, uji pewarnaan gram dan uji zona hambat bakteri. Karakteristik morfologi dan pengelompokan gram bakteri halofilik ekstrim diamati dibawah mikroskop binokuler model CX43RF dengan tipe kamera digital MDCE-5C. Uji aktivitas isolat bakteri halofilik ekstrim terhadap Salmonella sp. dilakukan dengan uji zona hambat menggunakan metode overlay dan difusi. Hasil penelitian diperoleh menunjukkan bahwa isolat bakteri halofilik esktrim mampu menghambat aktivitas Salmonella sp. dengan kisaran zona hambat berdiameter 4,28 mm dan 2,45 mm. Hasil penelitian ini diharapkan menjadi informasi awal untuk dipertimbangkan dalam pengembangan pemanfaatan bakteri halofilik ekstrim di bidang bioteknologi khususnya farmakologi laut kedepannya. Salmonella sp. in the waters can be detrimental to aquaculture, reduce the quality of captured fishery products and damage the quality of processed fishery products. Research needs to be done to overcome and prevent the activity of Salmonella sp. in some of the fisheries sectors. One of the efforts that can be done is to utilize the halophilic bacteria resources that live abundantly in the crystallization table water of traditional salt ponds as agents that inhibit the activity of these bacteria. This study aims to determine the potential of extremely halophilic bacteria cultured from Pamekasan-Madura traditional salt ponds as inhibitors of Salmonella sp. bacteria activity. The method used in this study consisted of several stages, namely isolation, purification, gram staining test, and bacterial inhibition zone test. Morphological characteristics and gram grouping of extremely halophilic bacteria were observed under a CX43RF binocular microscope with a digital camera type MDCE-5C. Activity test of extremely halophilic bacteria isolates against Salmonella sp. was carried out by testing the zone of inhibition using overlay and diffusion methods. The results showed that the extreme halophilic bacteria isolates were able to inhibit the activity of Salmonella sp. with a range of 4.28 mm and 2.45 mm diameter inhibition zones. The results of this study are expected to be initial information to be considered in the development of the use of extremely halophilic bacteria in the field of biotechnology, especially marine pharmacology in the future.
Kandungan Nutrien Pada Air Baku, Bozem, Kolam Peminihan Dan Meja Kristalisasi Di Tambak Garam Ary Giri Dwi Kartika; Makhfud Effendy; Arya Galin Fakhrul Islam Zain; Bahri Fadloli; Eka Nurrahema Ning Asih
Zona Laut : Jurnal Inovasi Sains Dan Teknologi Kelautan Volume 4, Nomor 1, Edisi Maret 2023
Publisher : Departemen Teknik Kelautan Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62012/zl.v4i1.25771

Abstract

Tambak garam terdiri dari beberapa kolam dengan fungsinya masing masing. Pada kolam kolam air tersebut terdapat biota mikro lengkap dengan nutrien yang mendukung asupan nutrisinya. Nutrien yang terakumulasi terlalu banyak dalam air akan mempengaruhi kualitas hasil produksi garam. Penelitian ini bertujuan untuk mengetahui kandungan nutrien yang terdiri dari nitrat, fosfat dan amonia serta pengaruhnya terhadap parameter kualitas air. Penelitian dilakukan pada 2 tambak garam di Kabupaten Bangkalan dan Pamekasan.  Pengambilan sampel air dilakukan di air baku, bozem, peminihan, dan meja kristalisasi  tambak garam yang kemudian sampel air tersebut dimasukan kedalam botol PET. Selanjutnya, sampel tersebut air dianalisis kandungan nitrat dengan metode brusin disulfanilat (SNI 06-2480-1991), asam askorbat untuk fosfat (SNI 06-6989.31-2005) dan alkalin sitrat untuk amonia (SNI 06-6989.30-2005). Hasil uji Kruskal-Wallis menunjukan adanya perbedaan kandungan nitrat, fosfat dan amonia  yang signifikan pada masing masing tiitk pengambilan sampel. Hal tersebut menunjukkan bahwa tiap kolam dengan kondisi kualitas air dan lingkungan sekitar mempengaruhi kandungan nutrien di air baku garam.
Potensi Ekstrak Lamun Enhalus acoroides Kering dan Basah Dari Perairan Sapeken-Madura Sebagai Antibakteri Vibrio parahaemolyticus Yuniar Mardiyanti; Eka Nurrahema Ning Asih; Fina Rohmatika; Siti Nihayatun Ni'amah
Juvenil Vol 5, No 2: Mei (2024)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v5i2.20998

Abstract

ABSTRAKBakteri Vibrio parahaemolyticus merupakan bakteri patogen yang memicu kegagalan panen budidaya udang di beberapa unit tambak budidaya udang di Madura. Salah satu kandidat bahan hayati laut yang bisa dijadikan sebagai agen antibakteri Vibrio parahaemolyticus adalah ekstrak lamun Enhalus acoroides. Perlu dilakukan telaah secara ilmiah untuk mengetahui konsentrasi ekstrak lamun ini dalam menghambat pertumbuhan bakteri Vibrio parahaemolyticus. Tujuan penelitian ini adalah mengidentifikasi perbedaan kemampuan antibakteri dari ekstrak Enhalus acoroides kering dan basah dengan konsentrasi yang berbeda terhadap pertumbuhan bakteri Vibrio parahaemolyticus selama 3 waktu pengamatan dan menganalisis konsentrasi terbaik ekstrak Enhalus acoroides kering dan basah dalam menghambat pertumbuhan bakteri Vibrio parahaemolyticus dari Perairan Sapeken Madura. Metode pengujian antibakteri menggunakan difusi cakram dan analisa statistik menggunakan One Way Anova dan uji Tukey HSD. Hasil uji antibakteri menunjukkan bahwa ekstrak Enhalus acoroides kering dan basah memiliki nilai tertinggi pada konsentrasi 80000 ppm pada masing-masing ekstrak dengan kategori lemah dengan kisaran zona hambat nilai 1.53±0,70 mm-2.15±0.91 mm pada lamun kering dan kategori sedang dengan kisaran zona hambat 2.35±0.13 mm-3.55±1.60 mm pada lamun basah. Konsentrasi 80000 ppm ekstrak Enhalus acoroides kering dan basah memiliki pengaruh yang signifikan dan termasuk konsentrasi terbaik sebagai antibakteri Vibrio parahaemolyticus dengan nilai signifikan (p0.05) sebesar 0.022 untuk lamun kering dan 0.010 untuk lamun basah.Kata kunci: Antibakteri, Vibrio parahaemolyticus, Enhalus acoroidesABSTRACTThe Vibrio parahaemolyticus bacteria is a pathogenic bacteria that triggers shrimp cultivation harvest failures in several shrimp cultivation pond units in Madura. One candidate for marine biological materials that can be used as an antibacterial agent for Vibrio parahaemolyticus is Enhalus acoroides seagrass extract. A scientific study needs to be carried out to determine the concentration of this seagrass extract in inhibiting the growth of Vibrio parahaemolyticus bacteria. This research aims to identify differences in the antibacterial ability of dry and wet Enhalus acoroides extracts with different concentrations against the growth of Vibrio parahaemolyticus bacteria during 3 observation periods and to analyze the best concentrations of dry and wet Enhalus acoroides extracts in inhibiting the growth of Vibrio parahaemolyticus bacteria from Sapeken Madura Waters. The antibacterial testing method uses disc diffusion and statistical analysis uses One Way Anova and the Tukey HSD Test. The antibacterial test results showed that dry and wet Enhalus acoroides extracts had the highest value at a concentration of 80,000 ppm in each extract with a weak category with a range of inhibition zone values of 1.53 ± 0.70 mm-2.15 ± 0.91 mm for dry seagrass and a medium category with a range of inhibition zone 2.35 ± 0.13 mm-3.55 ± 1.60 mm in wet seagrass. A concentration of 80000 ppm of dry and wet Enhalus acoroides extract has a significant effect and is among the best concentrations as an antibacterial for Vibrio parahaemolyticus with a significant value (p0.05) of 0.022 for dry seagrass and 0.010 for wet seagrass.Keywords: Antibacterial, Vibrio parahaemolyticus, Enhalus acoroides
DETEKSI INDIKASI ERITEMA PADA SEDIAAN HAND BODY LOTION DARI EKSTRAK LAMUN (Enhalus acoroides) DAN GONAD BULU BABI (Diadema setosum) Lailatul Badriyah; Eka Nurrahema Ning Asih; Siti Nihayatun Ni’amah; Reza Hidayah Ningrum; Yuniar Mardiyanti; Destin Retno Wulansari
Jurnal Perikanan Unram Vol 13 No 1 (2023): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v13i1.437

Abstract

Eritema adalah indikasi fisiologi berupa bercak kemerahan pada kulit akibat pemakaian hand body lotion yang mengandung bahan berbahaya pada kulit. Salah satu bahan hayati laut yang berpotensi sebagai anti eritema dalam sediaan hand body lotion adalah lamun (Enhalus acoroides) dan gonad bulu babi (Diadema setosum). Tujuan penelitian ini adalah untuk mengetahui persentase dan indeks indikasi eritema yang muncul dari 3 formulasi sediaan hand body lotion dari lamun dan gonad bulu babi pada kulit panelis. Metode dalam penelitian ini berupa eksperimen dengan merancang 3 formulasi sediaan hand body lotion, evaluasi fisik produk berupa uji organoleptik, dan uji in-vivo indikasi eritema dari ke 3 formulasi tersebut. Hasil penelitian menunjukkan bahwa dari 3 formulasi dari perbandingan ekstrak lamun dan gonad bulu babi yang dibuat yaitu F1 (2:1), F2 (1:2), F3 (3:3) dan kontrol F0 (0:0) didapatkan sebanyak 78% tidak terindikasi eritema pada 30 panelis. Sebanyak 16% panelis terindikasi sangat sedikit eritema (skala 2x1 cm), 6% panelis terindikasi eritema sedang serta 0% terindikasi eritema. Hasil uji organoleptik produk menunjukkan hand body lotion F1 berwarna celery tekstur 75% padat, F2 berwarna sage tekstur 50% padat, F3 berwarna olive tekstur 75% padat dan F0 berwarna putih tekstur 50% padat. Tingkat kesukaan panelis terhadap tekstur dan warna tertinggi pada F0 (Formula 0).
POTENSI EKSTRAK DAN SKRINING FITOKIMIA Caulerpa sp. SEBAGAI ANTIBAKTERI Vibrio parahaemolyticus DARI PERAIRAN SOCAH, BANGKALAN-MADURA Fina Rohmatika; Eka Nurrahema Ning Asih; Yuniar Mardiyanti; Siti Nihayatun Ni’amah
Jurnal Perikanan Unram Vol 13 No 4 (2023): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v13i4.557

Abstract

Prevelensi penyakit vibriosis yang menjadi penyebab kegagalan produksi budidaya udang vannamei pada dekade ini disebabkan oleh infeksi bakteri patogen Vibrio parahaemolyticus. Fenomena ini membutuhkan alternatif solusi untuk mengurangi resiko kegagalan panen salah satunya dengan menyediakan herbal kaya senyawa fitokimia berbahan sumberdaya hayati laut sebagai anti bakteri Vibrio parahaemolyticus misalnya anggur laut (Caulerpa sp.). Penelitian ini bertujuan untuk mengetahui senyawa bioaktif yang terdapat dalam ekstrak Caulerpa sp. dan potensi aktivitas antibakteri ekstrak Caulerpa sp. terhadap bakteri Vibrio parahaemolyticus. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi cakram dengan media Zobell Agar 2216E. Pengukuran zona hambat menggunakan jangka sorong dengan 3 waktu pengukuran (3x24 jam). Konsentrasi ekstrak Caulerpa sp. yang digunakan yaitu 10.000 ppm, 20.000 ppm, 40.000 ppm, 80.000 ppm, kontrol positif (kloramfenikol), kontrol negatif (aquades) dan kontrol tanpa perlakuan. Hasil uji fitokimia menunjukkan bahwa ekstrak Caulerpa sp. mengandung senyawa alkaloid, triterpenoid, dan saponin. Aktivitas antibakteri menunjukkan terdapat perbedaan nyata pengaruh konsentrasi ekstrak Caulerpa sp. terhadap Vibrio parahaemolyticus (Sig<0,05). Zona hambat tertinggi terdapat pada konsentrasi 80.000 ppm (kategori sedang) pada waktu pengamatan 24 jam (diameter zona hambat 4,22 mm ±0,22 mm).
Jenis Gram dan Morfologi Koloni Bakteri Air Baku Garam Ninik Agustina; Eka Nurrahema Ning Asih; Ary Giri Dwi Kartika
Jurnal Ilmu Kelautan Lesser Sunda Vol. 2 No. 1 (2022): Jurnal Ilmu Kelautan - Lesser Sunda
Publisher : Program Studi Ilmu Kelautan, Jurusan Perikanan dan Ilmu Kelautan, Fakultas Pertanian, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jikls.v2i1.44

Abstract

Halophilic bacteria are bacteria that can survive in environments with high salt levels, one of which is in salt ponds. Therefore, it is necessary to research to determine the morphology of halophilic bacteria for further use in the identification of bacterial species. This study aimed to determine the colony morphology and types of gram halophilic bacteria. Isolation of halophilic bacteria was carried out using the scatter method. Bacterial purification was carried out by streak method, then morphology identification and bacterial staining were carried out to determine the cell shape and gram of bacteria. The isolation results obtained 4 isolates from raw water (B.AB.1) and reservoir (B.B.1, B.B.2, and B.B.3). The results of the identification of the colony morphology of each bacterial isolate had different colors, shapes, colony edges, elevations, and surfaces. The results of the gram staining test showed that 2 isolates (B.B.1 and B.B.2) were gram-positive and 2 bacterial isolates (B.AB.1 and B.B.3) were gram-negative. The isolates of B.AB.1, B.B.2 and B.B.3 were in the form of bacilli, while the isolates of B.B1 were in the form of cocci.
Co-Authors Ary Giri Dwi Kartika Arya Galin Fakhrul Islam Zain Arya Galin Fakhrul Islami Zain Bahri Fadloli Bahri Fadloli Destin Retno Wulansari Destin Retno Wulansari Dewi Anugrah Fitri Dewi Anugrah Fitri Fina Rohmatika Fina Rohmatika Kartika Dewi Lailatul Badriyah Lailatul Badriyah Makhfud Efendy Makhfud Efendy Makhfud Effendy Ninik Agustina Reza Hidayah Ningrum Reza Hidayah Ningrum Salsabil Firda Nazhifan Siti Nihayatun Ni&#039;amah Siti Nihayatun Ni’amah Siti Nihayatun Ni’amah Sri Astutik Sri Astutik Yuniar Mardiyanti Yuniar Mardiyanti Yuniar Mardiyanti