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All Journal MEDIA MEDIKA INDONESIANA International Journal of Science and Engineering (IJSE) Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy)

Henna Rya Sunoko

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Author-ID : 366510

Engineering Health Professions Medicine & Pharmacology

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Articles

Peran Gen Polimorfik δ Asam Amino Levulinat Dehidratase pada Intoksikasi Pb Henna Rya Sunoko
MEDIA MEDIKA INDONESIANA 2008:MMI Volume 43 Issue 1 Year 2008
Publisher : MEDIA MEDIKA INDONESIANA

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ABSTRACTThe role of δ amino levulinic acid dehydratase polymorphism gene in lead intoxication.Background: Three distinct isozymes, designated ALAD 1-1, 1-2, and 2-2 are resulted from the expression of two common alleles, designated ALAD1 and ALAD2. The existence of this polymorphism gene whose product was implicated in the pathogenesis of lead toxicity suggested the potential for a genetically determined differential susceptibility. This study was attempted to investigate the role of ALAD polymorphism gene in lead intoxication showed by children blood lead levels.Methods: The study involved 54 children with 5-10-year-old, coming from Gebang Sari, Sekaran and Moro Demak. Cross-sectional design was adopted in this research. Blood lead level was measured by atomic absorption spectrophotometer. The ALAD polymorphism in exon 4 was determined by polymerase chain reaction (PCR) with restriction fragment length polymorphism, according to the methods described by Schwartz et al. Data were not statistically analyzed due to only two samples were ALAD2.Results: Two samples were ALAD2 (1-2 isozymes) with blood lead levels (BLL) of 24.50 ppb and 91.70 ppb respectivelly. The mean of ALAD2 BLL was about 48.60 ppb higher compared to that of ALAD1BLL.Conclusion: Individuals with ALAD2genotype had blood lead levels higher than those of individuals with the ALAD1 genotype, therefore, ALAD2genotype was much more susceptible to lead .Key Words: ALAD polymorphism gene, ALAD1, ALAD2, blood lead levelABSTRAKLatar belakang: ALAD 1-1, 1-2, dan 2-2, adalah tiga isozym yang berasal dari dua macam alel yaitu ALAD1 dan ALAD2. Keberadaan gen ALAD polimorfik dengan produknya ini, dalam patogenesis toksisitas Pb telah mengimplikasikan bahwa secara genetik sangat potensial untuk menyebabkan terjadinya perbedaan suseptabilitas terhadap Pb. Penelitian ini ditujukan untuk mempelajari peran gen ALAD polimorfik pada intoksikasi Pb yang ditunjukkan oleh tingkat kadar Pb darah.Metode: Penelitian mengikutsertakan 54 anak, umur 5-10 tahun, berasal dari Gebang Sari, Sekaran dan Moro Demak. Penelitian didesain secara cross-sectional. Kadar Pb darah diukur dengan alat atomic absorption spectrophotometer. ALAD polimorfik pada exon 4 dideterminasi dengan polymerase chain reaction (PCR) dengan restriksi “fragmentlength polymorphism” sesuai dengan metoda Schwartz et al. Analisis statistik tidak dilakukan sebab hanya ada dua sampel dengan ALAD2.Hasil: Ditemukan dua sampel anak dengan ALAD2 (1-2 isozymes), kadar Pb darah masing-masing 24,50 ppb dan 91,70 ppb. Rerata kadar Pb darah dengan ALAD2 lebih tinggi sekitar 48,60 ppb dibanding rerata kadar Pb darah dengan ALAD1.Simpulan: Genotipe ALAD2 lebih suseptibel terhadap Pb.

THE ANALYSIS, IDENTIFICATION, AND FORMULATION OF METALLOTHIONEIN EXTRACT AVAILABLE IN ROOTS, STEMS, LEAVES , FLOWERS , AND GRAINS OF RICE, CORNS, BEANS , AND SOYBEANS Budi Santosa; Henna Rya Sunoko; Andri S
International Journal of Science and Engineering Vol 10, No 1 (2016)
Publisher : Chemical Engineering Diponegoro University

Abstract –Vegetable materials are easily found around us but are frequently considered useless. The Metallothionein content in vegetable materials such in roots, stems, leaves, flowers, and fruit of rice, corns, beans, and soybeans has not been investigated before. Metallothionein protein has the role to bind heavy metals and serves as means of detoxification of heavy metals. This studi investigated to analyze, identify, and formulate metallothionein extracts from vegetable materials such as in roots, stems, leaves, flowers, and fruit of rice, corns, beans, and soybeans. The Experimental, each vegetable material available in roots, stems, leaves, flowers and fruit of rice, corns, beans, and soybeans was made into simplisia (crude medication) and then was processed into infuse. ELISA method was conducted as the measurement techniques upon protein level of each vegetable material infuse. The levels of Metallothionein protein available in vegetable materials were identified to determine the highest. The extract formula was taken from the highest levels of metallothionein. The result showed that the average metallothionein protein level from the entire vegetable materials was 0.62 ng: the highest in rice leaves of 1.4 ng and the lowest in bean flowers of 0.2 ng. The rice leaves were further formulated into metallothionein Extract. The analysis of metallothionein levels of vegetable materials such as in roots, stems, leaves, flowers, and fruit of rice, corns, beans and soybeans found that the highest level was in rice leaves of 1.354539 ng, and the lowest was in bean flowers of 0.22478. Key Words - Vegetable, metallothionein..

Zinc Supplementation Dosage Variations to Metallothionein Protein Level of Rattus Norvegicus Budi Santosa; Hertanto Wahyu Subagyo; Lisyani S; Henna Rya Sunoko
International Journal of Science and Engineering Vol 5, No 2 (2013)
Publisher : Chemical Engineering Diponegoro University

Zinc is an essential trace element involving in the activity of more than 300 enzymes and proteins of human body. One important role of zinc is to improve metallothionein protein binding heavy metals and functioning as heavy metal detoxification facilities. This research discusses the effect of zinc supplement on the improvement of metallothionein protein level. The in vivo test involving 28 rats categorized in 4 groups was performed. The experiments used randomized post test control group design. The 3 groups were daily supplemented by zinc in th concentration of 0.2 mg, 0.4 mg, and 0.8 mg. Whereas, the last group was let without zinc treatment. As an indicator the metallothionein protein level was checked after three weeks. The data was then evaluated by Anova an Bonferroni test in order to know the signicant of protein level difference among the groups. The result showed that the average of metallothionein protein level improved by increasing zinc suppplement with the 0.95 ±0.20; 1.28 ±0.19; 1.39 ±0.09; 1.91 ±0.3 ng/ml metallothionein per 0, 0,2, 0,4, 0,8.mg zinc added. Based on the ANOVA and Bonferroni test, indicated that the improvement was significant as shown with p value of 0.00

ANALISIS KANDUNGAN LOGAM BERAT TIMBAL (Pb), KADMIUM (Cd) DAN MERKURI (Hg) DALAM CUMI-CUMI (Loligo Sp.) YANG DIPEROLEH DARI TPI TAMBAK LOROK SEMARANG aqnes budiarti; rosmini rosmini; henna rya sunoko
Jurnal Ilmu Farmasi dan Farmasi Klinik JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 5 NO. 1 JUNI 2008
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACTThe research about analysis of heavy metal lead (Pb), cadmium (Cd) and mercury (Hg) in Octopuses (Loligo sp.) obtained from TPI Tambak Lorok Semarang has been carried out. This research was intended to identify heavy metals accumulating in octopuses (Loligo sp). The objects studied in this research were octopuses obtained from Tambak Lorok marketplace in Semarang. The sample for this research was taken by simple random sampling at three different points of locations. The locations 1, 2 and 3 were located each from the west, the north and the east, respectively, from the port. The contents of lead, cadmium and mercury in the octopuses’ bodies were measured using SSA (Atom-Absorption Spectrophotometer). The obtained data were then tested using One-way anava. The differences in metal contents were analyzed using Scheffe’s tests and then compared with the basic quality standard stipulated in the Decision Number 03725/B/SK/VII/1989 by the POM Directorate General The study based on the three different points of locations showed the following results. The average lead contents in the octopuses obtained from the points of locations 1, 2 and 3 were 0.29 mg/kg, 0.24 mg/kg and 0.25 mg/kg respectively. The average cadmium contents in the octopuses obtained from the points of locations 1, 2 and 3 were 0.04 mg/kg, 0.05 mg/kg and 0.06 mg/kg respectively. The average mercury contents in the octopuses obtained from the points of locations 1, 2 and 3 were 0.06 mg/kg, 0.07 mg/kg and 0.07 mg/kg respectively The lead, cadmium and mercury contents in the octopuses obtained from the three locations had not exceeded upper limit stipulated in the Decision Number 03725/B/SK/VII/1989 by the POM Directorate General. Based on the One-way ANOVA statistical tests on the lead contents, there was no significant difference among the three samples, but the statistical tests on the cadmium and mercury contents showed a significant difference among the three different points of locations relating to these heavy metal contents in the octopuses Keywords : SSA (Atomic-Absorption Spectrophotometer), Lead (Pb), Cadmium (Cd), Mercury (Hg), Octopuses (Loligo sp), Heavy Metal

Co-Authors AA Sudharmawan, AA Andri S Aqnes Budiarti Budi Santosa Budi Santosa Hertanto Wahyu Subagyo Lisyani S Rosmini Rosmini

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