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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Oto Rhino Laryngologica Indonesiana International Journal of Nasopharyngeal Carcinoma
Pudji Rahaju
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Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Medicine & Pharmacology

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Hubungan status nutrisi penderita karsinoma nasofaring stadium lanjut dengan kejadian mukositis sesudah radioterapi Fransiska Tricia; Pudji Rahaju; Rus Suheryanto
Oto Rhino Laryngologica Indonesiana Vol 42, No 1 (2012): Volume 42, No. 1 January - June 2012
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1131.729 KB) | DOI: 10.32637/orli.v42i1.40

Abstract

Background: Normal nutritional status is a balanced condition of nutritional intake and requirement in a person. Lack of nutrition in cancer patients promotes undesirable effects on various organs and systems of the body. Purpose: To determine the nutritional status differences in patients with advanced stage NPC type III WHO before and after radiotherapy,  and the relationship between nutritional status with mucositis occurance after radiotherapy. Method: Observational analytic study. Sampling was conducted by non-random purposive sampling technique with 10 subjects with NPC. The statistical analysis used paired sample Wilcoxon test and Spearman correlation test. Result: The study found significant differences between nutritional state regarding BMI, LOLA and transferrin before and after radiotherapy with p<0.05. Paired sample t test of BMI, LOLA, transferrin before and after radiotherapy demonstrated BMI p=0.000, LOLA p=0.001 and transferrin p=0.005. The paired sample t test for albumin before and after radiotherapy showed that radiotherapy did not cause significant decrease in albumin. Correlation test to determine the relationship among BMI, LOLA, albumin, and transferrin before radiotherapy with mucositis occurance showed BMI p=0.062, LOLA p=0.209, p=0.904 albumin, transferrin p=0.631 which meant that nutritional state has no corelation with mucositis occurance. While after radiotherapy it showed BMI p=0.122, p=0.209 LOLA, albumin, p=0.902 and transferrin p=1.000 which meant that nutritional state after radiotherapy has no corelation to the occurance of mucositis. Conclusion: Radiotherapy in patients with advanced-stage of NPC caused a significant difference in nutritional state before and after radiotherapy, but had no significant association with mucositis occurance. Keywords: nasopharyngeal carcinoma, radiotherapy, nutritional state, malnutrition, mucositis Abstrak :  Latar belakang: Status nutrisi normal menggambarkan keseimbangan yang baik antara asupan nutrisi dengan kebutuhan nutrisi. Kekurangan nutrisi pada penderita kanker memberikan efek yang tidak diinginkan terhadap struktur dan fungsi hampir semua organ dan sistem tubuh. Tujuan: Penelitian ini untuk mengetahui perbedaan status nutrisi penderita karsioma nasofaring (KNF) WHO tipe III   stadium lanjut   sebelum dan sesudah radioterapi, hubungan status nutrisinya dengan kejadian mukositis sesudah radioterapi. Metode: Penelitian ini adalah observasional analitik, dengan jumlah sampel 10 penderita KNF. Analisis statistik menggunakan paired sample t test dan uji korelasi Spearman. Hasil: Terdapat perbedaan yang bermakna antara status nutrisi dengan parameter Body Mass Index (BMI), lingkar otot lengan atas (LOLA) dan transferin sebelum dan sesudah radioterapi (BMI p=0,000, LOLA p=0,001 dan transferin p=0,005 dengan p<0,05). Pada paired sample t test albumin sebelum dan sesudah radioterapi menunjukkan nilai p=0,205 yang berarti bahwa radioterapi tidak menyebabkan penurunan albumin yang bermakna. Uji korelasi hubungan antara BMI, LOLA, albumin, dan transferin sebelum radioterapi dengan kejadian mukositis menunjukkan bahwa status nutrisi tidak berhubungan dengan kejadian mukositis (BMI p=0,062, LOLA p=0,209, albumin p=0,904, transferin p=0,631 dengan p>0,05). Uji korelasi hubungan antara BMI, LOLA, albumin, dan transferinsesudah radioterapi menunjukkan bahwa status nutrisi tidak berhubungan secara signifikan dengan kejadian mukositis sesudah radioterapi (BMI p=0,122, LOLA p=0,209, albumin p=0,902 dan transferin p=1,000 dengan p>0,05) Kesimpulan: Pemberian radioterapi pada penderita KNF stadium lanjut menyebabkan penurunan bermakna pada status nutrisi sebelum dan sesudah radioterapi, tetapi tidak berhubungan secara bermakna dengan kejadian mukositis sesudah radioterapi.  Kata kunci: karsinoma nasofaring, radioterapi, status nutrisi, malnutrisi, mukositis.
Hubungan mutasi gen ras dan p53 pada penderita karsinoma nasofaring dengan riwayat merokok Mohammad Dwijo Murdiyo; Cici Sunihapsari; Pudji Rahaju; Endang Retnoningsih; Johanes Bambang Soemantri
Oto Rhino Laryngologica Indonesiana Vol 43, No 1 (2013): Volume 43, No. 1 January - June 2013
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.713 KB) | DOI: 10.32637/orli.v43i1.14

Abstract

Background: Nasopharyngeal carcinoma (NPC) is a malignancy with multifactorial etiology involving Epstein Barr virus infection, genetic and environmental factors. Cigarette smoking is one of the environmental factors which have many compounds of carcinogen. Oncogene ras and tumor suppressor p53 gene play an important role in carcinogenesis. Mutation of these genes may contribute to NPC carcinogenesis. Purpose: To determine correlation between ras and p53 mutations in NPC patients with cigarette smoking history (quantity, duration, cumulative exposure). Methods: Thirty patients diagnosed as end stage type III WHO NPC were included in this cross sectional study. Thirty NPC biopsies were assessed for ras and p53 mutation by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The Fisher’s exact test and T test were used to analyse the correlation of ras, p53 mutation and cigarette smoking history. Results: Nras mutation was observed in 21(75%) subjects. One (3,33%) subject had H-ras mutation. There were no significant correlation between cigarette smoking history with N-ras (p=0,662) and H-ras (p=0,400) mutation, no significant correlation between N-ras, H-ras mutation with quantity, duration and cumulative exposure ofcigarette smoking (p>0.05). The p53 mutation was observed in 27 (93.1%). No significant correlation between cigarette smoking history and p53 mutation (p=1.000). No significant correlation between p53 mutation with quantity, duration and cumulative exposure of cigarette smoking (p>0.05). Conclusion: There were no significant correlation between cigarette smoking history and mutation of N-ras, H-ras and p53. N-ras, H-ras, p53 mutations were not correlated with quantity, duration and cumulative exposure of cigarette smoking.Keywords: nasopharyngeal carcinoma, ras, p53, cigarette smoking. ABSTRAKLatar belakang: Karsinoma nasofaring (KNF) memiliki etiologi multifaktorial yaitu infeksi virus EpsteinBarr, faktor genetik dan faktor lingkungan. Asap rokok adalah salah satu faktor lingkungan yang mengandungbahan karsinogen. Onkogen ras dan tumor suppressor gen p53 memiliki peran pada karsinogenesis. Mutasi gengentersebut dianggap berperan pada karsinogenesis KNF. Tujuan: Untuk mengetahui hubungan mutasi gen rasdan p53 pada penderita KNF dengan riwayat merokok. Metode: Studi cross sectional dengan subjek penelitian 30penderita KNF WHO tipe III stadium lanjut. Pemeriksaan mutasi gen ras dan p53 melalui pemeriksaan polymerasechain reaction (PCR) dan restriction fragment length polymorphism (RFLP). Analisa statistik menggunakan ujiFisher’s exact dan uji T. Hasil: Mutasi N-ras didapatkan pada 21(75%) subjek dari 28 subjek yang dapat diketahuimutasinya. Satu (3.33%) subjek mengalami mutasi H-ras dari 30 subjek. Tidak didapatkan hubungan bermaknaantara mutasi N-ras (p=0.662) dan H-ras (p=0.400) dengan riwayat merokok. Tidak ada perbedaan bermakna rataratajumlah, lamanya dan paparan kumulatif rokok antara subjek yang mengalami mutasi atau tidak mutasi N-rasdan H-ras (p>0,05). Mutasi p53 didapatkan pada 27 (93.15%) subjek dari 29 subjek. Tidak ada hubungan bermaknaantara riwayat merokok dengan terjadinya mutasi p53 (p=1.000). Tidak ada perbedaan bermakna rata-rata jumlah,lamanya dan paparan kumulatif rokok antara subjek yang mengalami mutasi atau tidak ada mutasi p53 (p>0,05).Kesimpulan: Tidak didapatkan hubungan bermakna antara riwayat merokok dengan terjadinya mutasi N-ras, Hrasdan p53. Tidak ada perbedaan bermakna rata-rata jumlah, lamanya dan paparan kumulatif rokok antara subjekyang mengalami dan yang tidak mengalami mutasi N-ras, H-ras dan p53.Kata kunci: karsinoma nasofaring, ras, p53, merokok.
CONTRIBUTION OF CANCER STEM CELLS TO THE METASTATIC MECHANISM IN POSITIVE LMP I WHO TYPE III NASOPHARYNGEAL CANCER PATIENT BEFORE AND AFTER NEOADJUVANT CHEMOTHERAPY Pudji Rahaju; Anis Eko Kristiawan; Endang Retnoningsih
INTERNATIONAL JOURNAL OF NASOPHARYNGEAL CARCINOMA Vol. 1 No. 03 (2019): International Journal of Nasopharyngeal Carcinoma
Publisher : TALENTA PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijnpc.v1i03.2057

Abstract

Introduction Nasopharyngeal cancer is the most common malignant tumour of the head and neck that caused high mortality. Most of patients came for treatment in late stage with enlargement of the neck lymphnode. Cancer stem cells are characteristic of stemness such as self-renewal and survival that caused tumour metastasis and recurrence. CD44, SOX2 and CCR7 are marker for NPC stem cells. Method Nine new patients NPC, WHO type III, positive LMP1 were examined for volume of the neck lymphnode, CD44, SOX2 and CCR7 expression before and after treatment chemotherapy cisplatin based. Staging determined by AJCC/UICC 2010. Result The results of this study showed that all subjects have increased SOX2 and CCR7 expression after treatment with statistically negative correlation. CD44 did not altered before and after treatment and cannot be evaluate. Correlation between alteration of the increase SOX2 and CCR7 expression and reduction of the volume have shown statistically positive correlation. Conclusion Neoadjuvant Chemotherapy Cisplatin based eliminated progenitor cells but did not for cancer stem cells in NPC. This result indicates that NPC with higher cancer stem cells must be more attention forward potential resistance and recurrence of the tumour. Although the have received all Stanford treatment
Hubungan kadar leptin serum dengan derajat rinitis alergi Ahmad Dian Wahyudiono; Endang Retnoningsih; Pudji Rahaju
Oto Rhino Laryngologica Indonesiana Vol 41, No 1 (2011): Volume 41, No. 1 January - June 2011
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.611 KB) | DOI: 10.32637/orli.v41i1.56

Abstract

Background: Allergic rhinitis is a global health problem that could impair the patient’s quality of life. Recent studies had showed the role of leptin, a hormone that produced by adipose tissue, on sensitization process which can increase the serum level of B cells and IgE. Purpose: To define the relationship between serum leptin level with the degree of allergic rhinitis based on ARIA and VAS.Methods: This study involved 38 subjects with cross sectional design. Statistical analysis included t-test, logistic regression and Kruskal-Wallis. Results: This study showed serum leptin level has correlation with the degree of allergic rhinitis based on ARIA (p<0.05), specifically on the intensity of allergic rhinitis (p<0.05), but not with the degree of allergic rhinitis based on VAS. Conclusion: Serum leptin level has a role on the degree of allergic rhinitis specifically on the intensity but not on the severity of allergic rhinitis symptoms. Controlling the serum leptin level can be considered as health promotion for patient with allergic rhinitis. Further research focusing on controlling serum leptin level for allergic rhinitis symptoms is recommended. Keywords: allergic rhinitis, serum leptin level, degree of allergic rhinitis   Abstrak :  Latar belakang: Rinitis alergi merupakan masalah kesehatan global dan dapat mengganggu kualitas hidup penderitanya. Beberapa penelitian telah menunjukkan peran leptin, hormon yang diproduksi oleh jaringan lemak, pada proses sensitisasi yang ditandai dengan kemampuan leptin meningkatkan sel B dan IgE. Tujuan: Penelitian ini bertujuan mengetahui hubungan kadar leptin serum dengan derajat rinitis alergi. Metode: Penelitian ini melibatkan 38 subjek dengan desain potong lintang untuk mengetahui hubungan kadar leptin serum dengan derajat rinitis alergi pada penderita rinitis alergi. Analisis statistik yang digunakan adalah uji t, uji regresi dan Kruskal-Wallis.Hasil: Penelitian ini menunjukkan bahwa kadar leptin serum berhubungan dengan derajat rinitis alergi berdasarkan ARIA (p<0,05) khususnya dengan intensitas keluhannya (p<0,05), namun tidak berhubungan dengan derajat rinitis berdasarkan VAS. Kesimpulan: Kadar leptin serum berhubungan dengan derajat rinitis alergi terutama pada intensitas keluhan dan bukan pada beratnya keluhan. Pengendalian kadar leptin serum dapat dipertimbangkan sebagai upaya memperbaiki kesehatan penderita rinitis alergi. Penelitian lebih lanjut yang menekankan pada pengendalian kadar serum leptin disarankan untuk mengendalikan keluhan rinitis alergi. Kata kunci: rinitis alergi, kadar leptin serum, derajat rinitis alergi
Effect of Tetrodotoxin from Crude Puffer Fish (Tetraodon fluviatilis) Liver Extract on Intracellular Calcium Level and Apoptosis of HeLa Cell Culture Natanael Untario; Titik Cinthia Dewi; M. Aris Widodo; Pudji Rahaju
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.04

Abstract

Cervical cancer is the third most commonly diagnosed cancer and fourth leading cause of women death with 8% of total death caused by cancer in women in 2008. Tetrodotoxin (TTX) is a potent neurotoxin found in inner organs puffer fish, with the specific mechanism of sodium channel blocking, and widely used for research purposes. Previous reports claimed that TTX has the capability of inhibiting the metastatic process of cancer and apoptotic effect. Studies also show that apoptosis is a process involving the increase of intracellular calcium level, yet the connection between TTX and increase of intracellular calcium level, therefore triggering apoptosis, has not been established. This is an experimental study with post test only control group design, carried out by exposing HeLa cell culture to a crude liver extract of a puffer fish species, Tetraodon fluviatilis. Crude puffer fish liver extract is administered into HeLa cell culture well in different concentrations 10-4, 10-2, and 10-1. Intracellular calcium level and apoptosis were then measured after 18 hours of incubation. Measurements of intracellular calcium level were done by using CLSM with Fura-2AM staining, and apoptosis by using flowcytometry with Annexin V/PI.  The result shows that there is a significant difference between samples both in intracellular calcium (p < 0.05) and apoptosis (p < 0,05). Both intracellular calcium and apoptosis levels are proportional to liver fish extract concentration. Pearson’s correlation test shows correlation between treatment and intracellular calcium levels (p = 0.000), between treatment and apoptosis (p = 0.002), but not between intracellular calcium and apoptosis (p = 0.05). These results suggest that TTX induces an increase in intracellular calcium level and apoptosis, but calcium pathway is not the sole cause of the apoptosis. 
Co-Authors Ahmad Dian Wahyudiono Anis Eko Kristiawan Cici Sunihapsari Endang Retnoningsih Endang Retnoningsih Fransiska Tricia Johanes Bambang Soemantri M. Aris Widodo Mohammad Dwijo Murdiyo Natanael Untario Rus Suheryanto Titik Cinthia Dewi