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All Journal BioEksakta : Jurnal Ilmiah Biologi Unsoed
Ajeng Arum Sari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Decolorization Of Indigosol Blue Dye Using Trametes versicolor F200 and Aspergillus sp Sunu Pertiwi; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.848 KB) | DOI: 10.20884/1.bioe.2020.2.2.1835

Abstract

The dyeing process of batik eventually produces much of wastewater. The difficult degradation and the dangers posed within the synthetic dyes are the main concerns in finding efficient wastewater treatment. Biological treatment has been known to be an effective technique of reducing or eliminating color intensity in wastewater. Fungi is one organism that can decompose many environmental pollutants. The aims of this research were to determine the ability of fungal isolates in decolorizing the synthetic dyes and analyzed which treatment has the highest decolorization percentage. Fungal isolates of Trametes versicolor F200 and Aspergillus sp. were used as a biological agent to decolorize of Indigosol Blue dye. The decolorization percentage was analyzed by spectrophotometer method. The result showed that T. versicolor F200 and Aspergillus sp. able to decolorize Indigosol Blue dye. The decolorization treatment of Indigosol Blue dyes using T. versicolor F200 showed the highest decolorization percentage reaching 97.21%.
OPTIMASI WAKTU INKUBASI DAN pH Ganoderma sp. DARI KEBUN RAYA BATURRADEN UNTUK DEKOLORISASI RBBR Maria Pricilia Gita; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.947 KB) | DOI: 10.20884/1.bioe.2020.2.1.1771

Abstract

Jamur pelapuk putih diketahui memiliki kemampuan untuk mendekolorisasi pewarna, salah satunya jamur Ganoderma sp. dari Kebun Raya Baturraden. Jamur tersebut mampu mendekolorisasi Remazol Brillliant Blue R (RBBR) yang bersifat toksik, mutagenik, karsinogenik, dan stabil terhadap perlakuan fisika maupun kimia. Proses dekolorisasi dapat dipengaruhi oleh faktor lingkungan seperti waktu inkubasi dan pH. Setiap jamur pelapuk putih memiliki waktu inkubasi dan pH optimum yang berbeda dalam mendekolorisasi pewarna. Penelitian ini bertujuan untuk mengetahui kemampuan isolat Ganoderma sp. dari Kebun Raya Baturraden dengan waktu inkubasi dan pH berbeda dalam mendekolorisasi pewarna RBBR, serta mengetahui variasi waktu inkubasi dan pH yang menunjukkan hasil terbaik. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan Perlakuan variasi pada penelitian ini terdiri dari waktu inkubasi yaitu 24, 48, 72, 96, dan 120 jam, serta pH 3, 4, 5, 6, dan 7. Hasil penelitian menunjukkan bahwa Ganoderma sp. dari Kebun Raya Baturraden mampu mendekolorisasi RBBR pada waktu inkubasi dan pH berbeda dengan persentase dekolorisasi 4,10% - 83,04%. Persentase dekolorisasi tertinggi ditunjukkan pada waktu inkubasi 96 jam dan pH 6, yaitu 83,04%. Hal tersebut membuktikan bahwa Ganoderma sp. dari Kebun Raya Baturraden memiliki waktu inkubasi optimum 96 jam, serta pH optimum 6 untuk mendekolorisasi RBBR.
Aspergillus sp. For Indigosol Blue and Remazol Brilliant Blue R Decolorization Fitria Ayudi Ulfimaturahmah; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.1795

Abstract

Synthetic dyes are artificial dyes manufactured by Industry and commonly used for the textile industry. These dyes had potentially caused an environmental problem. Many types of dyes are recalcitrant and have toxic properties for living organisms. It can be removed by decolorization method, especially a biological decolorization by fungi. Fungi were chosen due to the ability to degrade toxic components. Aspergillus sp. is the fungi which commonly used for dye decolorization. It might be caused that Aspergillus sp. is one type of fungi lived in the textile waste and expected not to die in the dye decolorization treatment. The purpose of this research was to investigate the ability of the mycelia pellets of Aspergillus sp to decolorized Indigosol Blue dye and Remazol Brilliant Blue R (RBBR) dye. This research showed that mycelial pellets of Aspergillus sp. had high activity of decolorization of Indigosol Blue dye up to 85.37% and RBBR dye up to 80.21% and caused low pH value after 24 hour incubation time compared to the control solution.
Aktivitas Enzimatik Isolat Jamur Auricularia sp., Trametes sp., dan Pholiota sp. pada Pewarna Remazol Brilliant Blue R dengan Variasi pH Agung Wiriat Putra Pratama Hadi; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.325 KB) | DOI: 10.20884/1.bioe.2020.2.1.1772

Abstract

Limbah dari industri tekstil berupa zat warna sintetik seperti Remazol Brilliant Blue R (RBBR) dapat menimbulkan pencemaran lingkungan akibat sifat zat warna RBBR yang toksik dan sulit terurai. Isolat Auricularia sp.,Trametes sp., dan Pholiota sp. memiliki enzim yang mampu mendekolorisasi komponen pewarna RBBR. Aktivitas enzim lignolitik seperti lakase yang dimiliki jamur mampu mengoksidasi pewarna tekstil. Aktivitas enzim lakase dipengaruhi oleh jenis jamur yang digunakan, pH, suhu, dan waktu inkubasi. Penelitian bertujuan untuk mengetahui aktivitas enzim lakase dari isolat jamur Auricularia sp., Trametes sp., dan Pholiota sp. dalam pewarna RBBR pada nilai pH berbeda, serta mengetahui isolat pada pH optimum yang memiliki aktivitas enzim lakase dalam pewarna RBBR. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Variabel bebas yang diamati adalah jenis isolat dan kondisi pH (4, 8, dan 12), sedangkan variabel tergantungnya adalah kemampuan isolat dalam menghasilkan enzim. Parameter utama yang diamati adalah aktivitas enzim Lakase. Parameter pendukung terdiri atas nilai bobot kering miselium. Hasil menunjukan ketiga isolat memiliki aktivitas enzim lakase dalam RBBR dengan pH berbeda. Aktivitas tertinggi terdapat pada Trametes sp. dalam RBBR dengan pH 4 yaitu sebesar 101,9 U/mL. Aktivitas terendah terdapat pada Aricularia sp. dalam RBBR dengan pH 4 sebesar 48,6 U/mL.
Biodegradasi Limbah Lindi Hitam, Acid Orange dan Acid Red menggunakan Jamur Trametes versicolor F200 Rismi Seftiani Jaitun; Nuraeni Ekowati; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.789 KB) | DOI: 10.20884/1.bioe.2019.1.2.1806

Abstract

Limbah lindi hitam dan zat warna azo (acid orangedan acid red) merupakan limbah berbahaya yang dapat mengkontaminasi ekosistem, sehingga perlu dilakukan pengolahan sebelum limbah dibuang ke ekosistem. Pengolahan secara fisik maupun kimiawi memiliki kelemahan diantaranya membutuhkan input energi tinggi, biaya yang mahal, penerapan yang terbatas dan menghasilkan produk lain yang bersifat toksik. Trametes versicolor dapat dijadikan sebagai agen biodegradasi, sebab menghasilkan anzim ligninolitik seperti Lakase (Lac), Manganese peroksidase (MnP) dan Lignin peroksidase (LiP).Jamur T. versicolor F200 mampu mendekolorisasi hingga 50%pada limbah lindi hitam; 90% pada Acid orange; dan 90% pada Acid red. Aktivitas enzim Lac, MnP dan LiP terbaik masing-masing yaitu 143,59 U/L (6 hari inkubasi), 33,3 U/L (6 hari inkubasi) dan 1167,42 U/L (awal inkubasi) pada limbah lindi hitam; 520,513 U/L) (awal inkubasi), 138 U/L (awal inkubasi) dan 358 U/L (awal inkubasi) pada acid orange; dan 408,3 U/L (awal inkubasi, 77 U/L (awal inkubasi) dan 228,871 U/L (akhir inkubasi). Kata kunci : Biodegradasi, Limbah lindi hitam, Trametes versicolor F200 dan acid orange dan acid red
Biodegradasi Bioplastik Berbasis Pati Menggunakan Isolat Fungi Indigenous Asal Tempat Pembuangan Akhir Gunung Tugel, Kabupaten Banyumas Jimmy Al Fa'is; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4605

Abstract

The use of plastic in Indonesia, especially as packaging, is very popular among the public, because it is practical and has many uses. Plastic consumption in 2020 even reached 67.8 million tons per day. Therefore, one way to overcome this problem is to use and produce biodegradable plastics or bioplastics. Biodegradation of bioplastics can be done with fungi. The potential place to find bioplastic degrading fungi is the Gunung Tugel Ex-Final Disposal Site (TPA). Considering the fact that bioplastiks have a fairly good level of degradation, in this study a starch-based biodegradation test will be carried out using isolats of indigenous fungi originating from Ex-TPA Gunung Tugel using the method of calculating the weight loss of bioplastics (weight loss). This study aims to obtain starch-based bioplastic degrading fungi isolats from Ex-TPA Gunung Tugel, Banyumas Regency and determine the highest biodegradability ability of starch-based bioplastics by fungal isolats from Ex-TPA Gunung Tugel, Banyumas Regency. The results showed that isolats of indigenous fungi that had the potential to degrade starch-based bioplastics from Ex-TPA Gunung Tugel, Banyumas Regency were obtained from the genus Aspergillus (GT2 isolate) and Penicillium (GT1, GT3, GT4, GT5, and GT6) isolate. The highest biodegradability of starch-based bioplastics was found by fungi from the genus Aspergillus, namely isolate GT2 with a weight loss of 21.84%. The biodegradation occurs due to the activity of endoamylase, exoamylase, and glucanase enzymes produced by fungi. The observation of the texture of the bioplastic resulted in a significant change in color, namely from the white bioplastic sheet to brownish. These observations also showed the attachment of fungal mycelium on the surface of the bioplastik accompanied by a hollow and uneven surface of the bioplastikc. Keywords : Biodegradation, Starch-based bioplastik, Indigenous Fungi, TPA.
Co-Authors Agung Wiriat Putra Pratama Hadi Fitria Ayudi Ulfimaturahmah Jimmy Al Fa'is Maria Pricilia Gita Nuraeni Ekowati Ratna Stia Dewi Rismi Seftiani Jaitun Sunu Pertiwi