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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BioEksakta : Jurnal Ilmiah Biologi Unsoed
Nuraeni Ekowati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Variasi Genetik Berdasarkan Penanda Molekular Random Amplified Polymorphic DNA Pada Jamur Shiitake (Lentinula edodes) Nuraeni Ekowati; Rina Sri Kasiamdari; Nursamsi Pusposendjojo; C.J. Soegihardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.97

Abstract

Penelitian ini bertujuan mengetahui variasi genetik jamur Lentinula edodes asal Malang, Cianjur, Lembang, dan Yogyakarta serta mendapatkan primer terseleksi untuk identifikasi L. edodes secara molekular. Penelitian dilakukan di Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta. Tahapan penelitian terdiri atas ekstraksi DNA dari miselium empat isolat L. edodes dan satu sampel outgroup (Pleurotus ostreatus), amplifikasi DNA dengan teknik PCR-RAPD menggunakan delapan jenis primer (OPA 1, OPA 2, OPA 3, OPA 4, OPA 7, OPA 8, OPA 9 dan OPA 10), elektroforesis menggunakan gel agarosa dan pengamatan pita DNA dengan UV transluminator. Data pita DNA dianalisis dengan software NTSYSpc21 untuk menentukan tingkat similaritas, jarak genetik dan untuk mengkonstruksi dendrogram berdasarkan metode UPGMA. Hasil penelitian menunjukkan bahwa semua primer yang digunakan dapat mengamplifikasi DNA sampel dan satu diantaranya (OPA 4) tidak dapat menunjukkan adanya polimorfisme pada keempat isolat. Ukuran fragmen DNA teramplifikasi berkisar antara 1291774 bp. Berdasarkan hasil penelitian dapat disimpulkan bahwa antara isolat L. edodes asal Malang, Cianjur, Lembang dan Yogyakarta terdapat variasi genetik dengan jarak genetik antara 7886%. Polimorfisme tertinggi (83,33%) diperoleh menggunakan primer OPA 2.
isolasi dan identifikasi jamur endofit pada tanaman moringa (moringa oleifera Lam.) Hasnadhiazahra Rohadi; Nuraeni Ekowati; Moh Ilyas
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.738 KB) | DOI: 10.20884/1.bioe.2019.1.2.1804

Abstract

Moringa oleifera Lam. is a small, perennial tree which belongs to moringaceae family. This plant is widely known for their nutritional and medicinal purposes. They can associate with microorganisms showing beneficial symbiosis called as endophytic microorganism. Endophytic fungi is microorganism capable of living within the plant tissues without harming their host. This study was aimed for obtaining pure culture of fungal endophytes from M. oleifera and then identifying them based on morphological identification. The morphological identification was done by observing both macro- and micro- morphological characteristics of the endophytic fungi. Twenty endophytic fungi were isolated from lamina, petiole and stem of M. oleifera. The genus obtained were Cladosporium, Colletotrichum, Fusarium, Phomopsis, Phyllosticta, white mycelia sterilia and black mycelia sterilia (Dematiaceae). Keywords: endophytic fungi; identification; isolation; morphology; Moringa oleifera Lam.
Detection of Psychotropic Compound in Coprophilous Fungi in District of Baturraden Banyumas Regency Safira Amanda; Aris Mumpuni; Nuraeni Ekowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.4245

Abstract

Coprophilous fungi or dung loving fungi are a group of fungi adapted to life on dung and fecal pellets of herbivorous animals. Coprophilous fungi contain psychotropic compounds. Psychotropics compounds are substances or drugs, both natural and synthetic, not narcotics, which have psychoactive properties through a selective influence on the central nervous system which causes distinctive changes in mental activity and behavior. Chemical Spot Test still remain an important tool for the preliminary identification of illicit drugs and other psychotropic compound in spite of developments in instrumental technology and the increased portability of this technology which enables its use in the field. Banyumas Regency is a very potential area as a habitat for coprophilous fungi, specifically in Baturraden District because there are many cattle farms where the dung is where the fungi grow, also the climate is suitable for fungal growth. make an inventory and identify the coprophilous fungi found in District of Baturraden Banyumas Regency and to detect the presence of psychotropic compound in the fungi. The research will be conducted using purposive random sampling and Color Test or Chemical Spot Test analysis. The obtained data is analyzed descriptively by comparing with Atlas of The Munsell Color System. This research obtained seven genera of coprophilous fungi i.e: Coprinellus sp., Coprinopsis sp., Entoloma sp., Gymnopus sp., Lepiota sp., Parasola sp. and Stropharia sp. that discovered in two cattle farms in Baturraden District.
Aplikasi Jamur Pelapuk Putih pada metode Biopulping berbahan dasar Limbah Daun Kayu Putih (Melaleuca leucadendron) Syifa Mubarrak; Aris Mumpuni; Nuraeni Ekowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3386

Abstract

biopulping limbah daun kayu putih lama dan limbah daun kayu putih baru dan mengetahui JPP terbaik untuk proses biopulping limbah daun kayu putih. Penelitian menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL). Perlakuan berupa menginokulasikan JPP (Phanerochaete chrysosporium, Ganoderma lucidum, dan Pleurotus tuberregium), tiap perlakuan diulang sebanyak 3 kali. Variabel penelitian terdiri atas variabel bebas berupa jenis JPP yang berbeda dan umur limbah, variabel terikat berupa kemampuan biodelignifikasi JPP pada limbah daun kayu putih. Parameter utama yang diamati adalah kadar lignin dan kadar selulosa sebelum dan sesudah masa inkubasi daun kayu putih. Parameter pendukung yang diamati adalah berat limbah sebelum dan sesudah inkubasi, pH, dan Berat Media.Hasil penelitian menunjukkan bahwa terjadi penurunan kadar lignin dan selulosa pada seluruh perlakuan. Hasil rata-rata penurunan kadar lignin terbesar yakni pada perlakuan jamur P. tuberregium terhadap limbah daun kayu putih lama sebesar 0,014%. Penurunan kadar selulosa akhir tertinggi pada perlakuan jamur terbesar adalah pada jamur G. lucidum terhadap limbah daun kayu putih lama mengalami rata-rata penurunan selulosa terbesar 3,22%.
Karakterisasi Isolat Khamir Gf1 Terhadap Kondisi Asam Saluran Pencernaan Unggas Awwaluz Zahroh Mahya Ainillah; Nuraeni Ekowati; Ade Erma Suryani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.833 KB) | DOI: 10.20884/1.bioe.2020.2.1.1745

Abstract

Probiotik merupakan mikroorganisme yang dapat memperbaiki mikroekologi usus yang berdampak positif terhadap kesehatan inang. Kandidat probiotik merupakan mikroorganisme yang memiliki potensi sebagai probiotik, seperti menghasilkan enzim fitase sehingga mampu mengoptimalkan kondisi saluran pencernaan. Salah satu mikroorganisme sebagai kandidat probiotik adalah khamir penghasil enzim fitase. Khamir penghasil fitase telah diisolasi oleh BPTBA-LIPI Yogyakarta asal makanan fermentasi tradisional Indonesia yaitu gathot.Pengujian khamir fitase sebagai kandidat probiotik dilakukan dengan menstimulasikan kondisi saluran pencernaan seperti kondisi asam yaitu pada pH rendah dan kondisi asam lambung. Kondisi pH yang rendah dan asam lambungmerupakan suatu penghalang masuknya mikroorganisme ke saluran gastrointestinal, oleh karena itu pengujian terhadap asam dilakukan untuk mengetahui karakteristik mikroorganisme yang akan digunakan sebagai kandidat probiotik. Pengujian dilakukan secara invitro dengan menguji ketahanan khamir pada kondisi asam dengan nilai pH 2 dan 3 serta kondisi asam lambung dengan menggunakan pepsin 0,3%. Hasil penelitian menunjukkan bahwa isolat khamir GF1 asal makanan tradisional fermentasi mampu tahan terhadap kondisi asam karena mampu mempertahankan viabilitasnya pada pH 3 dan kondisi asam lambung.
Biodegradasi Limbah Lindi Hitam, Acid Orange dan Acid Red menggunakan Jamur Trametes versicolor F200 Rismi Seftiani Jaitun; Nuraeni Ekowati; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.789 KB) | DOI: 10.20884/1.bioe.2019.1.2.1806

Abstract

Limbah lindi hitam dan zat warna azo (acid orangedan acid red) merupakan limbah berbahaya yang dapat mengkontaminasi ekosistem, sehingga perlu dilakukan pengolahan sebelum limbah dibuang ke ekosistem. Pengolahan secara fisik maupun kimiawi memiliki kelemahan diantaranya membutuhkan input energi tinggi, biaya yang mahal, penerapan yang terbatas dan menghasilkan produk lain yang bersifat toksik. Trametes versicolor dapat dijadikan sebagai agen biodegradasi, sebab menghasilkan anzim ligninolitik seperti Lakase (Lac), Manganese peroksidase (MnP) dan Lignin peroksidase (LiP).Jamur T. versicolor F200 mampu mendekolorisasi hingga 50%pada limbah lindi hitam; 90% pada Acid orange; dan 90% pada Acid red. Aktivitas enzim Lac, MnP dan LiP terbaik masing-masing yaitu 143,59 U/L (6 hari inkubasi), 33,3 U/L (6 hari inkubasi) dan 1167,42 U/L (awal inkubasi) pada limbah lindi hitam; 520,513 U/L) (awal inkubasi), 138 U/L (awal inkubasi) dan 358 U/L (awal inkubasi) pada acid orange; dan 408,3 U/L (awal inkubasi, 77 U/L (awal inkubasi) dan 228,871 U/L (akhir inkubasi). Kata kunci : Biodegradasi, Limbah lindi hitam, Trametes versicolor F200 dan acid orange dan acid red
Optimasi Konsentrasi Glukosa dan Waktu Inkubasi Terhadap Bobot β-Glukan Jamur Schizophyllum commune Tri Rahayu Apriyani; Nuraeni Ekowati; Nuniek Ina Ratnaningtyas
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4601

Abstract

The main purpose of this study was to determine the effect of the interaction between glucose concentration and incubation time on the growth of fungi S. commune and also the weight of β-glucan was used to determine the best glucose concentration and incubation time for the growth of fungi S. commune and β-glucan production. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in glucose concentration (G) with three levels, namely 10 g/L (G1), 20 g/L (G2), and 30 g/L (G3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (I1). , incubation time of 25 days (I2), and incubation time of 30 days (I3). Variables which used in this research independent and dependent variables. The independent variables were glucose concentration and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that glucose concentration and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. Glucose concentration of 30 g/L and incubation time of 25 days was the optimum glucose concentration and incubation time for the growth of S. commune fungus, and also glucose concentration of 30 g/L and incubation time of 25 days was the optimum glucose concentration and incubation time for β-glucan production of S. commune.
Pengaruh pH dan Waktu Inkubasi Berbeda Terhadap Pertumbuhan dan Produksi β-Glukan Schizophyllum commune Amalia Sofia Maharani; Nuraeni Ekowati; Nuniek Ina Ratnaningtyas
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4594

Abstract

The main purpose of this study are to determine the optimum value of pH and incubation time on S. commune's growth, and also the optimum value of pH and incubation time on β-glucan production of S. commune. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in pH (P) with three levels, namely pH 5 (P1), pH 6 (P2), and pH 7 (P3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (W1). , incubation time of 25 days (W2), and incubation time of 30 days (W3). Variables which used in this research independent and dependent variables. The independent variables were pH and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that pH and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. The value of pH 6 and incubation time of 25 days was the optimum pH and incubation time for the growth of S. commune fungus, and also pH 5 and incubation time of 25 days was the optimum pH and incubation time for β-glucan production of S. commune.
Co-Authors Ade Erma Suryani Ajeng Arum Sari Amalia Sofia Maharani Aris Mumpuni Awwaluz Zahroh Mahya Ainillah C.J. Soegihardjo Hasnadhiazahra Rohadi Moh Ilyas Nuniek Ina Ratnaningtyas Nursamsi Pusposendjojo Rina Sri Kasiamdari Rismi Seftiani Jaitun Safira Amanda Syifa Mubarrak Tri Rahayu Apriyani