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Sari Budi Moria Sembiring
Research Institute for Mariculture, Gondol, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PHENOTYPIC AND GENOTYPIC PERFORMANCES OF BLACK TIGER SHRIMP, Penaeus monodon HAVING FAST GROWTH TRAITS Haryanti Haryanti; Ahmad Muzaki; Ida Komang Wardana; Fachrudin Fachrudin; Sari Budi Moria Sembiring; I Gusti Ngurah Permana
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.932 KB) | DOI: 10.15578/iaj.4.2.2009.101-108

Abstract

Shrimp breeding research focusing on fast growth traits is important to develop better phenotypic and genotypic characters of shrimp spawners. The aim of this research was to evaluate shrimp breeding technology to produce fry having fast growth traits. Selection was initiated from the breeding of wild shrimp spawners (F-0) based on full sib mating, probiotics, biosecurity, and viral diseases diagnosis. Viral disease diagnoses were performed using 7 viruses (TSV, WSSV, IHHNV, YHV, BP, MBV, and HPV) provided in the IQ-2000 kit. The result showed that 11 families of the first generation (F-1) shrimp were phenotypically varied (big, regular and small size). Number of fry from the first generation resulted from phenotypic selection that have fast growth trait ranged between 1.99% and 4.49% of the total fry population. Genotypic performance of the shrimp broodstock (F-0) and the first generation (F-1) showed different genetic variations. Heterozigosity values were 0.2872±0.0047 (female) and 0.5487±0.0103 (male) for shrimp broodstock (F-0), 0.704±0.0261 for the first generation (F-1) having fast growth trait, and 0.6224±0.023 for F-1 having slow growth. Gene markers of fast growth trait were indicated by the genes with molecular weights of 1,025; 1,280; and 1,325 basepairs and having different DNA sequences compared to that of slow growth shrimp.
ASPECTS OF LEOPARD CORAL GROUPER (Plectropomus leopardus) REPRODUCTION IN INDONESIA Retno Andamari; Sari Budi Moria Sembiring; Gusti Ngurah Permana
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.853 KB) | DOI: 10.15578/iaj.2.1.2007.51-57

Abstract

Leopard coral grouper, Plectropomus leopardus is one of the most economically important finfish fish in Indonesia and the demand for the grouper is rapidly increasing in Asia and the Pacific. Grouper exports from Bali were 1,613 mt in 2001, 2,082 mt in 2002 and 2,861 mt in 2003. Understanding the reproductive biology of fishes is an important component in developing mariculture and in the management of capture fisheries. This study reports on the reproductive biology of 86 coral groupers collected from various locations in Indonesia. The length and weight of these fish were recorded and related to gonad development. There was a strong relationship between length and weight; weight being proportional to the length raised to the power (b value) 3.2. As the value of b was greater than 3, this suggests that growth is allometric. Histological analysis 73% of the fish were immature, 19% were in transition from females to males, only 4% were male, and only 2 fish (2%) had mature gonads: these were female. The sex of 2 fish could not be determined. From these data it can be seen that the leopard coralgrouper has asynchronous gonad development. The two fish that were mature contained 343,980 and 429,259 oocytes and three distinct sizes of oocytes could be found. This suggests that the grouper is a multiple spawner. If fish are required for brood stock, this study has shown that only those with a length greater than 35 cm in standard length should be taken from the wild.
Co-Authors Ahmad Muzaki Fachrudin Fachrudin Gusti Ngurah Permana Haryanti Haryanti I Gusti Ngurah Permana Ida Komang Wardana Retno Andamari