Angela Mariana Lusiastuti
Research Institute for Freshwater Aquaculture

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COMBINATION VACCINES AGAINST KOIHERPES VIRUS AND Aeromonas hydrophila CO-INFECTION IN KOI AND COMMON CARP Angela Mariana Lusiastuti; Hessy Novita; Lila Gardenia; Taukhid Taukhid; Habil Sven M. Bergmann
Indonesian Aquaculture Journal Vol 15, No 2 (2020): (December, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.2.2020.93-102

Abstract

Co-infections occur when hosts are infected by two or more pathogens, either simultaneous or as a secondary infection. This research aimed to determine the best compositions of vaccine combinations and their protective efficacies against pathogens co-infection. This research was conducted in two stages. Firstly, surveys were conducted in three research areas: infected, high risk of infection, and virus free areas. Samples (three to five fish per pool) were collected from three fish farms per area. The basic antibody titer of fish from each farm was checked before and after vaccination as well as after the virus challenge in combination with the PCR result. The second stage of the research was conducted in the laboratory. Carp and koi fish were used to determine optimal vaccine combination and dosage for oral application. The results of this research showed that combination of KHV: Aeromonas hydrophila vaccines in the ratio of 1:2 and vaccine volume of 3 mL via the oral application gave higher titer antibody and efficacy against KHV and A. hydrophila. In conclusion, the combined vaccine offers an effective means of preventing the diseases, decreasing fish mortality, and simplifying the immunization schedule, which will eventually increase the overall health of farmed fish and benefit fish farmers and service extension officers. This research recommends that further development of the combined vaccines should be carried out, for example, overcoming the technical difficulties in its manufacturing.
EFEKTIVITAS KOMBINASI PROBIOTIK MIKROENKAPSULASI MELALUI PAKAN UNTUK PENGENDALIAN PENYAKIT MOTILE AEROMONADS SEPTICEMIA PADA IKAN LELE, Clarias gariepinus Angela Mariana Lusiastuti; Septyan Andriyanto; Reza Samsudin
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.416 KB) | DOI: 10.15578/jra.12.2.2017.179-186

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas kombinasi probiotik mikroenkapsulasi melalui pakan dalam pengendalian penyakit pada ikan lele, Clarias gariepinus. Penelitian dilakukan mulai dari penyiapan kombinasi probiotik Bacillus cereus ND2 dan Staphylococcus lentus L1K, mikroenkapsulasi probiotik, uji probiotik mikroenkapsulasi, serta uji dosis secara in vivo. Proses mikroenkapsulasi menggunakan BUCHI mini spray dryer dengan suhu inlet 131°C-133°C dan suhu outlet 65°C-70°C. Pakan yang digunakan adalah pakan buatan dengan kadar protein 38% yang dicampur dengan probiotik mikroenkapsulasi sesuai dengan dosis perlakuan dan diberi putih telur 2% sebagai binder. Perlakuan yang dilakukan adalah membandingkan secara in vivo probiotik mikroenkapsulasi (PM) dan probiotik non-mikroenkapsulasi (PNM), dan dilanjutkan pengujian dosis melalui pakan untuk memperoleh dosis terbaik dari aplikasi probiotik mikroenkapsulasi. Parameter pengamatan berupa tingkat sintasan, biomassa, kadar hematokrit, hemoglobin, respiratory burst activity, dan total bacterial count. Hasil penelitian menunjukkan bahwa suplementasi kombinasi probiotik mikroenkapsulasi Bacillus cereus ND2 dan Staphylococcus lentus L1K pada pakan lebih efektif, dan dengan dosis 2% menunjukkan hasil yang lebih baik dalam meningkatkan sintasan hingga 97,33%, biomassa dan respons imun (NBT assay, hematocrit, hemoglobin) ikan lele terhadap A. hydrophila.This study was aimed to know the effectivity of microencapsulated probiotic combinations administered through diet to control motile aeromonads septicemia disease in catfish (Clarias gariepinus). The research stages consisted of preparation probiotics, microencapsulation of probiotics and probiotic microencapsulation testing in vivo. BUCHI mini spray dryer was used in the microencapsulation process with inlet temperature ranged between 131°C-133°C and outlet temperature ranged between 65°C-70°C. The diets used in the research were pelleted feeds with a protein content of 38% and mixed with different dosages of microencapsulated probiotics. Egg albumen (2%) was used to bind the mixture. The effectivity of microencapsulated and non-microencapsulated feeds was compared in order to find an optimum microencapsulation dosage. The observed parameters were survival rate, biomass, hematocrit, hemoglobin, respiratory burst activity, and total bacterial count. The results showed that the supplementation of microencapsulated probiotic Bacillus cereus ND2 and Staphylococcus lentus L1K in the diets was more effective at a dose of 2%, showing better survival rate (97.33%), biomass gain, and immune responses (NBT assay, hematocrit, hemoglobin) against A. hydrophila in catfish Clarias gariepinus.
EVALUASI PEMBERIAN PROBIOTIK BACILLUS PADA MEDIA PEMELIHARAAN TERHADAP LAJU PERTUMBUHAN DAN PERUBAHAN HISTOPATOLOGI IKAN LELE DUMBO (Clarias gariepinus) YANG DIINFEKSI Aeromonas hydrophila Angela Mariana Lusiastuti; Mohammad Faizal Ulkhaq; Widanarni Widanarni; Tri Heru Prihadi
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.736 KB) | DOI: 10.15578/jra.11.2.2016.171-179

Abstract

The aim of this study was to evaluate the addition of a probiotic Bacillus on culture medium through growth rate and histopathological change in African Catfish who was infected by Aeromonas hydrophila. The study consisted of five treatments such as the addition of probiotic Bacillus ND2, Bacillus P4I2, Bacillus ND2 + Bacillus P4I2 (Kom), positive control (K+) and negative control (K-) (without probiotic addition). African Catfish (13.354±2.8 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila  (103 CFU/mL) were added once in earlier treatment (except for the negative control) as assumption that A. hydrophila will shape up quorum sensing in unfortunate condition. The result showed that the highest spesific growth rate in the treatment of Bacillus ND2 probiotics (1,708 ± 0.114%). Histopathology showed that damaged of liver dan kidney in probiotics treatment were lightner than positive control (K+).  The addition of Bacillus P4I1 (104 CFU/mL) efective to increased the spesific growth rate of African Catfish and prevent the damage of liver and kidney of African Catfish was infected by Aeromoniasis.