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Andi Tenriulo
Research Institute for Coastal Aquaculture, Maros
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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CLONING OF ProAV PROMOTER ISOLATED FROM TIGER PRAWN Penaeus monodon Andi Parenrengi; Alimuddin Alimuddin; Sukenda Sukenda; Komar Sumantadinata; Muhammad Yamin; Andi Tenriulo
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.086 KB) | DOI: 10.15578/iaj.4.1.2009.1-7

Abstract

Promoter is a specific DNA sequence involved in the transcription of a particular gene. It is usually located in the upstream of the gene they regulate. Isolation and characterization of promoter is essentially needed in order to establish the sequence analysis and transcription factor that are used in the regulation of gene expression. The research was conducted to analyze the characteristics of Penaeus monodon anti viral gene promoter (ProAV) towards generation of auto-transgenic tiger prawn, P. monodon. ProAV promoter was isolated by PCR (Polymerase Chain Reaction) method and the purified DNA fragment was cloned into pGEM-T Easy cloning vector. The promoter sequence was characterized by using BLAST-N and Genetyx version 7 softwares. The results showed the success in isolating a promoter from tiger prawn of 368 bp in length. BLAST-N analysis showed that the sequence of isolated promoter has high similarity (95%-98%) compared to the other promoters in the GeneBank. The study revealed the existence of important transcription factors (TATA box, MRE, TCF-1, and other potential regulatory elements) are identified in the promoter sequence.
GENETIC VARIABILITY AND POPULATION STRUCTURE OF GROUPER (Epinephelus suillus) FROM MAKASSAR STRAIT AND BONE BAY, SOUTH SULAWESI, INDONESIA Andi Parenrengi; Andi Tenriulo
Indonesian Aquaculture Journal Vol 3, No 2 (2008): (December 2008)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.808 KB) | DOI: 10.15578/iaj.3.2.2008.77-87

Abstract

Random Amplified Polymorphic DNA (RAPD) was employed to determine the genetic variability and population structure of grouper (Epinephelus suillus) from Makassar Strait and Bone Bay, South Sulawesi, Indonesia. Genomic DNA was isolated from preserved muscle tissue using Phenol-Chloroform technique. Among 24-screened arbitrary primers, ten primers (OPA-02, OPA-06, OPA-08, OPA-10, OPA-15, OPA-16, OPA-17, OPA-18, OPA-19 and CA-05) were selected to generate RAPD fingerprinting of grouper populations. The ten primers generated a total of 212 fragments (loci) and 120 polymorphic fragments in their size ranging from 250 to 2,500bp. The high polymorphism (60%) was obtained from Makassar population followed by Bone (59%) and Pare-Pare populations (50%). Similarity index of individuals was 0.86±0.07 for Pare-Pare, 0.80±0.11 for Makassar and 0.82±0.07 for Bone population. Fifteen fragments from ten primes were identified as species-specific markers of E. suillus. The UPGMA cluster analysis showed that the dendrogram seemed to be clustered according to their geographical location, where Pare-Pare population was genetically closer to Makassar population (D=0.20) than to Bone population (D=0.24).
Co-Authors Alimuddin Alimuddin Andi Parenrengi Komar Sumantadinata Muhammad Yamin Sukenda Sukenda