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EFFECTS OF SYNTHETIC ASTAXANTHIN, CHLORELLA, AND SPIRULINA SUPPLEMENTATION IN DIETS ON GROWTH AND PIGMENTATION OF KURUMOI RAINBOWFISH, Melanotaenia parva Nina Meilisza; Muhammad Agus Suprayudi; Dedi Jusadi; Muhammad Zairin Jr.; I Made Artika
Indonesian Aquaculture Journal Vol 15, No 2 (2020): (December, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.2.2020.67-75

Abstract

Several studies have recommended the supplementation of astaxanthin in the Kurumoi rainbowfish diet to enhance its color and growth. However, knowledge regarding the effects of naturally-sourced and synthetically-made carotenoids in fish diets is currently limited. This study’s objective was to compare the growth and color performances of Melanotaenia parva by supplementing fish feed with synthetic astaxanthin and natural carotenoids sourced from Chlorella and Spirulina. A total of 12 fish (weight of 1.27 ± 0.02 g and total length of 4.70 ± 0.07 cm) were stocked at a density of one fish per liter. Basal feed (B) was used as the control feed. The experimental feeds were: B added with different doses of synthetic astaxanthin (Carophyll® Pink 10% water-soluble) from low to higher doses as follows: 0.6 g kg-1 (AS-L), 2.6 g kg-1 (AS-O), and 5.1 g kg-1 (AS-H); and B added with natural carotenoids of Chlorella sp. (Ch) and Spirulina sp. (Sp) of 8.6 g kg-1 and 5.5 g kg-1, respectively. The experimental diets were given at satiation for 56 days at 8 am and 3 pm. The study results showed that the addition of synthetic astaxanthin at a dose of 2.6 g kg-1 could increase the fish growth up to 12% with carotenoid deposition in the fish fin of three times higher than that of the treatments without synthetic astaxanthin. This dose was considered the optimal dose to increase the fish’s growth performance and pigmentation compared with the high dose of 5.1 g kg-1. Despite having the same nutrient composition, natural carotenoids in Chlorella and Spirulina did not produce better results compared to the low dose of synthetic astaxanthin of 0.6 g kg-1.
Sosialisasi Tanggap Darurat Pencegahan Covid-19 di Kepulauan Talaud I Made Artika; Mutmainnah A. Umar
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. Khusus 2 (2021): April 2021
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.885 KB)

Abstract

Since the World Health Organization or WHO declared that the Coronavirus disease (Covid-19) is a pandemic, the President of the Republic of Indonesia and the Regional Government and their staff have worked hand in hand to make some tactical steps to prevent the spread of this virus. The public must be informed on the dangers of Covid-19, through creation of special protocols which are expected to prevent the spread of this virus. The purpose of this activity was to conduct socialization directly or indirectly in providing education, convincing, and providing an overview of the dangers of the Covid-19 Virus and applying health protocols to the community in Melonguane District, Talaud Islands Regency, North Sulawesi Province. This activity took place from July 7 to August 28, 2020. The results of this activity showed that the people in the Talaud Islands have understood the importance of using good and correct masks when outside the home and also the use of hand soap or hand sanitizer which can help people to avoid this dangerous virus. Keywords: covid-19, emergency response, Kepulauan Talaud, Melonguane
Peningkatan Kesadaran Masyarakat Menghadapi Pandemi Covid-19 di Desa sukawati, Kabupaten Gianyar, Bali I Made Artika; Cok Istri Agung T Apriliyanti; Sang Ayu Putu Didi K Agustini; Ni Putu Asri Ratna Suhita; Ni Putu Peggy Liliyani; Sang Ayu Made Sri Tandewi; Ni Kadek Sintya Anggreni; I Nyoman Rai Widiartha Kesuma
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. Khusus 2 (2021): April 2021
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.969 KB)

Abstract

Sukawati Village is one of the tourist villages located in Sukawati, Gianyar, Bali. Sukawati Village has the potential that can be developed optimally if the role of the community, Sukawati Village officials, village youths, and farmer groups can unite in carrying out and developing the existing potential. Before the KKN-T program which was carried out in Sukawati Village, several problems had not been resolved by the village and the community, such as the minimal publications regarding Sukawati Village, limitations in marketing and financial strategies, and the existence of the Covid-19 pandemic which was the biggest problem. hampers economic and tourism activities. The activities carried out during the KKN-T in Sukawati Village are based on the main problems faced. The activities carried out received appreciation and support from village officials, the community, and village youth. The activities carried out produce each output based on the intended target. Activities carried out are more focused on community empowerment, such as the implementation of training in the fields of economy, technology, and health. Activities in the health sector include training in making soap and hand sanitizers as a means of dealing with the Covid-19 pandemic situation. For the economic sector, namely the socialization of financial strategies and marketing strategies. Activities in the field of technology include training village officials in creating village websites. The results obtained from this activity are in the form of increased sensitivity to the environment and surrounding communities, caring for each other, helping each other to help advance Sukawati Village during the pandemic and post-Covid-19 pandemic. We pack the training provided and make it in the form of a learning video so that in the future it can be used as a reference by the community and can be accessed on YouTube, Whatsapp groups, and Facebook in Sukawati Village. Keywords: Sukawati Village, liquid soap, hand sanitizer, website, marketing, covid-19
In silico phylogenetic, physicochemical, and structural characteristics of phytase enzyme from ten Aspergillus species Ridwan Putra Firmansyah; Shobiroh Nuur Alimah; I Made Artika; Popi Asri Kurniatin
Menara Perkebunan Vol. 92 No. 1 (2024): 92(1), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i1.559

Abstract

Phytic acid is a chemical compound consisting of inositol and phosphoric acid and is an antinutrient compound found in monogastric poultry feed ingredients made from cereal crops. Phytase hydrolyzes phosphoester bonds in phytic acid, releasing inorganic phosphate and phosphate esters. Aspergillus is a genus of molds that produce phytase and has been widely used in phytase production because they are easy to culture. This study aims to compare the structures, physicochemical characteristics, and phylogenetic relationships of phytases from several species of Aspergillus in silico as an initial screening step in obtaining the most suitable phytase to be used in poultry feed. Phylogenetic trees were constructed using MEGA 11 and physicochemical characteristics were analyzed using ProtParam. Protein structures were modeled with AlphaFold. The phytase structures were then docked with phytic acid using the YASARA Structure. The results showed that phytase 1QFX from Aspergillus niger, P34755 from A. awamori, and D5HQ11 from A. ficuum have very high similarity in terms of phylogenetics, sequences, physicochemical characteristics, and protein structures. The docking results from the three phytase structures showed that phytase 1QFX has the most negative ΔG value and the lowest Kd, which indicated the highest affinity to the phytic acid substrate. This research concludes that among the three phytase structures that have been compared and docked with phytic acid, phytase 1QFX from A. niger is the most suitable to be applied to poultry feed.