Anang Hari Kristanto
Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

KERAGAMAN GENETIK DAN BIOREPRODUKSI EMPAT POPULASI IKAN MATA MERAH (Puntius orphoides Valenciennes, 1842) Wahyulia Cahyanti; Fera Permata Putri; Sri Sundari; Anang Hari Kristanto
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.15.3.2020.141-149

Abstract

Ikan mata merah merupakan ikan konsumsi yang berkerabat dekat dengan ikan tawes yang terdistribusi luas di kawasan tropis. Analisis keragaman genetik dan performa reproduksi ikan mata merah, baik jantan maupun betina belum tersedia secara lengkap dan valid. Tujuan penelitian ini adalah untuk menentukan keragaman genetik ikan mata merah dari berbagai lokasi, dan mengkaji bioreproduksinya. Ikan sampel yang diperoleh dari nelayan dan pengumpul di empat lokasi berbeda yaitu: Jawa Barat (Tasikmalaya dan Cianjur), Jawa Tengah (Purwokerto), dan Jawa Timur (Umbulan, Pasuruan), ditampung di Balai Benih Ikan lokal, kemudian ditransportasikan ke Instalasi Plasma Nutfah, Cijeruk, Bogor. Sampel ikan dari berbagai ukuran digunakan untuk analisis keragaman genetik melalui analisis morfometrik dan RAPD, sedangkan untuk kajian bioreproduksi ikan yang digunakan adalah calon dan induk ikan mata merah jantan dan betina. Hasil analisis menunjukkan bahwa keragaman genetik populasi Purwokerto dan Umbulan memiliki perbedaan dengan nilai sharing component intraspesies tertinggi, yaitu 100,0%. Polimorfisme empat populasi ikan mata merah berkisar antara 1,92%-17,30% dengan kisaran tingkat heterozigositas 0,0088-0,0678. Populasi Purwokerto memiliki jarak genetik terjauh (0,0678) dari populasi lainnya. Pada pengamatan kinerja reproduksi hanya dua populasi yang matang gonad dan bisa dipijahkan, yaitu populasi Tasikmalaya dari 10 ekor matang gonad, tiga ekor berhasil memijah, dan Cianjur dari sembilan ekor matang gonad, tiga ekor berhasil memijah dengan nilai derajat pembuahan kedua populasi sebesar 100% dan derajat penetasan yang masih cukup tinggi Tasikmalaya 84,32 ± 7,38a% dan Cianjur 73,15 ± 3,78a%. Volume sperma ikan jantan diperoleh sebanyak 0,1 mL; dengan jumlah spermatozoa sebanyak 75 x 108 sel.Red-eye fish is a fish species closely related to tawes/silver barb/Java barb and widely distributed in tropical regions. As of now, information on genetic diversity and reproductive performance of red-eye fish, both male and female, have not yet been completed. Thus, this study was conducted to determine the genetic diversity of red-eye fish populations from different locations and study their bioreproduction. Live fish were obtained from fishermen and collectors in four different areas namely West Java (Tasikmalaya and Cianjur), Central Java (Purwokerto), and East Java (Umbulan, Pasuruan). The collected fish were temporarily stored in each local fish seed center before transported to the Germplasm Installation, Cijeruk, Bogor. Fish samples of various sizes were used for genetic diversity analysis through morphometric analysis and RAPD. In bioreproduction observation, the fish used were prospectively matured and matured male and female of red-eye fish. The results of the genetic study showed that the Purwokerto and Umbulan populations have genetic diversity differences with the highest intraspecies sharing component value of 100.0%. The polymorphism of four red-eye fish populations ranged from 1.92%-17.30% with a range of heterozygosity levels of 0.0088-0.0678. Purwokerto population has the farthest genetic distance (0.0678) from the other populations. The observation of reproductive performance found only two populations having mature gonad fish and could subsequently be artificially spawned. The populations were Tasikmalaya population with ten mature gonad fish, of which three fish successfully spawned and Cianjur population with nine mature gonad fish, of which three successfully spawned. The fertilization rate of the two spawned populations was 100%. The hatching rateof eggs from Tasikmalaya and Cianjur populations were relatively high of 84.32 ± 7.38a% and 73.15 ± 3.78a%, respectively. The maximum volume of sperm produced by each male fish from both populations was 0.1 mL, with the total number of spermatozoa of 75-108 cells.
PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN BAUNG (Hemibagrus nemurus) KETURUNAN G-1, G-2, DAN G-3 Irin Iriana Kusmini; Anang Hari Kristanto; Ani Widiyati; Fera Permata Putri
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.861 KB) | DOI: 10.15578/jra.14.4.2019.201-211

Abstract

Ikan baung (Hemibagrus nemurus) telah berhasil didomestikasi oleh Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Bogor. Hasil penelitian domestikasi, populasi Cirata memiliki fekunditas dan pertumbuhan yang lebih bagus, populasi ini dikembangkan hingga tiga generasi (G1, G2 dan G3) namun, ada informasi terbatas mengenai perbandingan antara perkembangan gonad dari berbagai generasi ikan. Tujuan penelitian ini adalah untuk mengukur pertumbuhan dan mengamati perkembangan gonad generasi pertama (G-1), kedua (G-2), dan ketiga (G-3). Parameter yang diukur dan diamati meliputi pengukuran panjang, berat, laju pertumbuhan spesifik, morfologi dan berat gonad, diameter oosit, dan indeks gonad somatik (IGS). Pengambilan sampel dilakukan setiap dua bulan dengan mengumpulkan sepuluh sampel ikan dari setiap generasi. Jaringan gonad disiapkan secara histologis dan diwarnai larutan asetokarmin sebagai zat pewarna. Pengamatan menggunakan mikroskop dengan perbesaran 4 x 10. Hasil penelitian menunjukkan bahwa pada usia 222 hari, gonad jantan dan betina secara fisik masih dalam bentuk benang halus. Namun, karakteristik sekunder pada ikan jantan seperti papila sudah mulai tampak meski tidak terlalu jelas. Pada usia 283 hari, ukuran oosit berkisar antara 0.025-0.05 mm. Pada usia 345 hari, telur mulai terlihat dengan ukuran oosit G-1, G-2, dan G-3 masing-masing berkisar antara 0.59 ± 0.24, 0.39 ± 0.15, dan 0.48 ± 0.15 mm. Setelah usia 406 hari, perkembangan gonad mencapai TKG-III dengan diameter rata-rata oosit G-1, G-2, dan G-3 berkisar antara 1.13 ± 0.11, 0.92 ± 0.18, dan 1.11 ± 0.10 mm dengan IGS dari G-1, G-2, dan G-3 dari 8.20%, 0.98 %, dan 4.8%, masing-masing. Tidak ada perbedaan yang signifikan antara diameter oosit G-1, G-2, dan G-3 (P£0,05). Keturunan kedua (G-2) merupakan generasi terbaik untuk dijadikan kandidat budidaya. Asian redtail catfish (Hemibagrus nemurus) has been successfully domesticated by the Research Institute for Freshwater Aquaculture and Fisheries Extension, Bogor. The results of the domestication of Cirata population have better fecundity and growth, this population has been developed for up to three generations (G1, G2 and G3) however, there is limited information regarding the comparison between the ovarian development of different generations of the fish]. This study’s aim was to measure the growth and observe the ovarian development of the first (G-1), second (G-2), and third generations (G-3). The parameters measured and observed included the measurements of length, weight, specific growth rate, morphology and gonad weight, oocyte diameter, and somatic gonad index (IGS). The sampling was carried out every two months by collecting ten fish samples of each generation. Gonad tissues were histologically prepared and stained asetocarmine solution as the coloring agent. The observations used a microscope with a magnification of 4 x 10. The results of the study showed that at the age of 222 days, the male and female gonads were physically still in the form of fine threads. However, secondary characteristics in male fish such as papillae have begun to appear though not very clear. At the age of 283 days, oocytes’ sizes ranged between 0.025-0.05 mm. At the age of 345 days, the eggs were starting to be visible with the oocyte sizes of G-1, G-2, and G-3 ranged between 0.59 ± 0.24, 0.39 ± 0.15, and 0.48 ± 0.15 mm, respectively. After the age of 406 days, the development of gonads reached gonad maturating level III with the average diameter of the oocytes of G-1, G-2, and G-3 ranged between 1.13 ± 0.11, 0.92 ± 0.18, and 1.11 ± 0.10 mm with the IGS of G-1, G-2, and G-3 of 8.20%, 0.98%, and 4.8%, respectively. There was no significant difference among the oocyte diameters of G-1, G-2, and G-3 (P£0.05). The 2nd generation (G2) is the best generation to be a candidate aquaculture.