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HENDIG WINARNO
LABORATORIUM BAHAN KESEHATAN PATIR-BATAN
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Uji Aktivitas Inhibisi Isolat Kuersitrin dari Benalu Masisin Dendrophthoe pentandra (L.) Miq. terhadap Sel Leukemia L1210 ERMIN KATRIN W.; RISMA MARISI TAMBUNAN; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 1 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Parasitic plant [Dendrophthoe pentandra (L.) Miq.] on the masisin [Rhodomyrtus tomentosa (Aiton) Hassk.] was traditionally used for cancer treatment in Central Kalimantan. Maceration of dried Dendrophthoe pentandra using ethanol followed by ethyl acetate produced ethanol and ethyl acetate extracts which exhibit inhibitory activity against leukemia L1210 cells with IC50 of 17.3 and 20.9 µg/mL, respectively. Fractionation of ethyl acetate extract by column chromatography yielded 7 fractions (F1~F7). Inhibitory activity test on fractions F1~F7 against leukemia L1210 cells showed inhibitory activity with IC50 0f 19.9, 19.0, 20.7, 16.0, 13.7, 12.5, and 17.4 µg/mL, respectively. During evaporation of F5 by rotary evaporator, a yellowish precipitate was observed and identified as quercitrin (quercetin 3-O-rhamnoside). Inhibitory activity test on quercitrin against leukemia L1210 cell showed that the crystal has an IC50 of 14.7 µg/mL. Separation of F7 by semipreparative HPLC reverse phase column yielded isolate A and B which exhibited inhibitory activity on leukemia L1210 cells with IC50 of 15.0 and 14.4 µg/mL, respectively. Subsequent solvent partition of ethanol extract into ethyl acetate-water and separation of ethyl acetatesoluble portion by column chromatography produced 7 fractions (F8~F14). Further separation of F14 by column chromatography yielded quercitrin and isolate C which has not been identified yet.
Sitotoksisitas terhadap Sel Leukemia L1210 dan Profil Kromatogram dari Serbuk Temu Putih Curcuma zedoaria (Berg) Rosc. yang Diradiasi ERMIN KATRIN W.; JOHANS RICHARD ALBERT; SWASONO R. TAMAT; SUSANTO SUSANTO; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 1 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

White turmeric Curcuma zedoaria (Berg) Rosc. is useful for treating cancer disease. It is very susceptible to microbial, therefore gamma irradiation technique to reduce microbial contamination is necessary in order to extend the storing period. Gamma irradiation on white tumeric was carried out by cobalt-60 source at doses of 5, 7.5, 10, and 15 kGy. Then they gradually were maeerated with n-hexane, ethyl acetate, and ethanol. The purpose of this research was to study the effect of gamma irradiation by observing parameters on the cytotoxic activity of ethyl acetate extract and active fraction of white tumeric rhizome against L1210 leukemia cells, also profiles of thin layer and HPLC chromatograms of active fraction as anticancer agent. Ethyl acetate extract of irradiated and control samples were the most active to inhibit the growth of L1210 leukemia cells with IC50 value of 4.71 ug/mL. Ethyl acetate extracts from irradiated and control samples were fractionated using column chromatography and obtained 7 fractions (Fr), respectively. Fraction 3 of control sample was the most active fraction with IC50 values 1.43 µg/ ml. The IC50 value of Fr 3 decreased with increasing irradiation doses and chromatogram profiles of radiated samples with doses of > 5 kGy were changed. The maximum radiation dose for white turmeric preservation is 7.5 kGy, at this dose its anticancer efficacy was maintained.
Co-Authors ERMIN KATRIN W. JOHANS RICHARD ALBERT Risma Marisi Tambunan SUSANTO SUSANTO Swasono R. Tamat