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Swasono R. Tamat
Pusat Radioisotop dan Radiofarmaka BATAN
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Produksi dan Penetapan Kadar B-glukan dari Tiga Galur Saccharomyces cerevisiae dalam Media Mengandung Molase Kusmiati Kusmiati; Swasono R. Tamat; S. Nuswantara; Nita Isnaini
JURNAL ILMU KEFARMASIAN INDONESIA Vol 5 No 1 (2007): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Beta-glucan extracted from the cell membrane of Saccharomyces cerevisiae has been used as food and medical ingredients. Molase as a waste-product of the cane sugar industry, containing 4-9% of glucose and other nutrients, has been investigated to substitute glucose in the YPG fermentation media of Saccharomyces cerevisiae, and to improve the yield of B-glucan by three strains of Saccharomyces cerevisiae (RTA, RN4, and SC). Beta-glucan and protein concentration were determined by spectrophotometric method at a 490 nm and 750 nm respectively. The results showed that molase can be used as glucose-substitute in the YPG media for the production of B-glucan by three strains of Saccharomyces cerevisiae. The best yield for each strain was as follows: RTA strain in a media containing molase (8% v/v glucose equivalent) produced 61,79% w/w of B-glucan; RN4 strain in a media containing molase (1% v/v glucose equivalent) produced 98,42% w/w of B-glucan; SC strain in a media containing molase (2% v/v glucose equivalent) produced 56,48% w/w of B-glucan. One-way Anova followed by the Tukey-Bonferroni test indicated that molase can be used as substitute of glucose source in the YPG fermentation media, and significantly increased the B-glucan yield by all the three strains of Saccharomyces cerevisiae, as well as reducing the protein contents. The highest ß-glucan yield (98,42% w/w) was attained by the RN4 strain in a media containing molase (1% v/v glucose equivalent) with a protein impurity of only 10,53% w/w, while the RN4 and RTA strains produced a higher B-glucan yield than that of the SC strain.
Penetapan Kadar Simultan Deksametason dan Metilprednisolon dengan Metode Kromatografi Gas Spektrometri Massa melalui Derivatisasi Swasono R. Tamat; Ernawati Ernawati; Linda Rosalina; Sonny Pujianto
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 2 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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This investigation is intended to find a simultaneous assay method for glucocorticosteroids declared as doping compound by the International Olympic Committee. Experiments were carried out in the determination of dexamethason and methylprednisolon in urine matrix by gas chromatography-mass spectrometry (GC-MS) using various derivatisation reagents, reaction temperature and time. Selection of the best derivatisation reagent was based on the separation of total ion chromatogram (TIC) and appearance of three characteristic ions, m/z 305, 345,680 or m/z 206,456, 662 for dexamethasone; and m/ z 279, 369, 459 or m/z 147, 424, 644 for methylprednisolone. Dexamethasone and methylprednisolone derivates in the GC-MS spectra were identified by characteristic ions of m/z 305 (dexamethasone) and m/ z 279 (methylprednisolone). The best derivatisation yield was achieved by a combination of TMSI - [BSTFA-TMCS (99:1)](2:1) reacted at 90°C for 90 minutes, which produced a linear correlation between concentration (0-150 ng/ml ) and ratio of quantitation-ion abundance of standard compound/ion abundance of D3-testosterone internal standard with r= 0.9937 for dexamethasone and r=0.9823 for methylprednisolone; high precision CV=12.14% (dexamethasone) and CV=13.87%(methylprednisolone); high recovery for both dexamethasone and methylprednisolone, and LOD values of 4,44ng/ml (dexamethasone) and 1.80ng/ml (methylprednisolone). The method can be used for simultaneous quantitative assay of glucocorticosteroids, and has been validated for ten other glucocorticosteroid compounds.
Aktivitas Antioksidan dan Toksisitas Senyawa Bioaktif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal Swasono R. Tamat; Thamrin Wikanta; Lina S. Maulina
JURNAL ILMU KEFARMASIAN INDONESIA Vol 5 No 1 (2007): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Research on the antioxidative activity and toxicity of the bioactive substance of the green seaweed Ulva reticulata Forsskal extract had been carried out. The bioactive substance of the green seaweed Ulva reticulata Forsskal was extracted using methanol. The phytochemistry test on the methanol extract showed that the extract contained triterpenoid compound. The methanol extract was then partitioned using n-hexane-water (1:1) and chloroform-water (1:1). Each of the extract was then dried using freeze dryer. The antioxidative activity test of each fraction against free radical 1,1-diphenyl-2-picrilhydrazil (DPPH) showed that the water extract has the highest activity with IC 30 of 365,95 ug/ml. The toxicity test of each fraction against brine shrimp Artemia salina (Brine Shrimp Lethality Test, BSLT) showed that the chloroform extract showed the highest activity with LCs of 250,67 ug/ml. The water extract was then fractionated through silica column chromatography. The activity test of each fraction against DPPH and BSLT showed that fraction 1 has the highest activity with LCs of 100 ug/ml and ICs of 270,31ug/ ml. Identification using gas chromatography – mass spectrometric method showed that the antioxidative substance presence in the active fraction was probably nonyl phenol (C, H2,0).
Identifikasi, Uji Antioksidan, dan Uji Toksisitas Senyawa Bioaktif dalam Caulerpa sertularioides (Vahl.) C. Agard. Thamrin Wikanta; Swasono R. Tamat; Magdalena Sinta Maryati
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Caulerpa sertularioides (Vahl.) C. Agard. is a green seaweed species in the Caulerpaceae family which has been used for medication. Extraction of bioactive compounds from Caulerpa sertularioides have been carried out and screened based on the antioxidative property against DPPH and on toxicity test by the brine shrimp lethality test (BSLT) method. Phytochemical screening of the methanol extract indicated the presence of a triterpen. The methanol extract was partitioned in sequence with n-hexanewater (1:1) and chloroform-water (1:1). The IC50 value of the water extract was found 327 ug/ml and the LCs value was 115 ug/ml as compared to the ICs of vitamin C positive control of 21 ug/ml. Fractination of the water extract in a silica gel column with various ratio of chloroform-methanol did not produce distinct separation. Fractions were analyzed on TLC and fractions of similar components were collected. Fraction I has IC50 value of 326 ug/ml and LC50 value of 68 ug/ml. Further separation and purification of fraction I on a preparative thin layer chromatographic system using chloroform-methanol (50:1) produced five isolates bearing antioxidative activity. The third isolate that showed the strongest activity, when analyzed using a GC-MS system indicating seven compounds, but only [(2-fluorophenyl)methyl)-1H-purin-6-amine that may have an antioxidative activity.
Kapasitas Pengikatan Iodida dan Iod pada Karbon Aktif Konvensional dan Terbrominasi Sunarhadijoso Soenarjo; Swasono R. Tamat; Ade Saputra
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Related to the activities in a radioisotope processing laboratory at CDRR, BATAN, active carbon has a great potency and a role as adsorber for radioisotope immobilizing agent in process and installation systems. KAKEN Corporation, a CDRR's collaboration partner in Japan, has introduced a new type of active carbon namely KAKEN Brominated Active Carbon (KBAC) which is claimed to have an anionic exchange character. The presented experiment was thus performed to determine the iodide and iodine binding capacities of the KBAC resin as well as comparing to those of the conventional active carbon, which was also provided by KAKEN (KCAC). The experiment had been done using solutions of natural iodide and iodide - iodine mixtures. The binding capacities were determined by iodometry and iodatometry. In general the results obtained showed that the iodide-binding capacity of KBAC was higher than that of KCAC, but that the iodine-binding capacity of KCAC was higher. The KBAC showed that the binding capacity of iodide from iodide solutions was smaller than that from iodide-iodine samples, while the KCAC showed the reverse.
Co-Authors Ade Saputra Ernawati Ernawati Kusmiati Kusmiati Lina S. Maulina Magdalena Sinta Maryati Nita Isnaini Rosalina, Linda S. Nuswantara Sonny Pujianto Sunarhadijoso Soenarjo Thamrin Wikanta