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Thamrin Wikanta
Pusat Riset Pengolahan Produk dan Sosial Ekonomi Kelautan
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Aktivitas Antioksidan dan Toksisitas Senyawa Bioaktif dari Ekstrak Rumput Laut Hijau Ulva reticulata Forsskal Swasono R. Tamat; Thamrin Wikanta; Lina S. Maulina
JURNAL ILMU KEFARMASIAN INDONESIA Vol 5 No 1 (2007): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Research on the antioxidative activity and toxicity of the bioactive substance of the green seaweed Ulva reticulata Forsskal extract had been carried out. The bioactive substance of the green seaweed Ulva reticulata Forsskal was extracted using methanol. The phytochemistry test on the methanol extract showed that the extract contained triterpenoid compound. The methanol extract was then partitioned using n-hexane-water (1:1) and chloroform-water (1:1). Each of the extract was then dried using freeze dryer. The antioxidative activity test of each fraction against free radical 1,1-diphenyl-2-picrilhydrazil (DPPH) showed that the water extract has the highest activity with IC 30 of 365,95 ug/ml. The toxicity test of each fraction against brine shrimp Artemia salina (Brine Shrimp Lethality Test, BSLT) showed that the chloroform extract showed the highest activity with LCs of 250,67 ug/ml. The water extract was then fractionated through silica column chromatography. The activity test of each fraction against DPPH and BSLT showed that fraction 1 has the highest activity with LCs of 100 ug/ml and ICs of 270,31ug/ ml. Identification using gas chromatography – mass spectrometric method showed that the antioxidative substance presence in the active fraction was probably nonyl phenol (C, H2,0).
Gambaran Histopatologi Pankreas Tikus Hiperglikemia setelah Mengkonsumsi K-Karagenan dan í-Karagenan Lestahari Rahayu; Rahma Damayanti; Thamrin Wikanta
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 2 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Research on the effect of K-carrageen and i-carrageen feeding on the representation of pancreatic B-cell histopathology of hyperglycemic rat has been carried out. Rats were previously damaged by aloxan monohydrat induction. There were 6 groups of rats, each group containing 5 animals. Group I normal control, group II negative control, group III 1st positive control, group IV 2nd positive control, group V K-carrageen treatment, group VI i-carrageen treatment. Group I, II, IV, V, and VI, were given orally, while group III was given subcutaneously. All the treatments were given during 15 days. All the rats were then killed and pancreatic hystopathology were carried out. The results revealed that aloxan monohydrat induction resulting the rat pancreatic weight reduction in all treatment groups. The ratio of rat body weight to the pancreatic weight was not significant difference in all treatment groups. Insulin injection was unable to increase the size of Langerhans islet and unable to support the recovery process of pancreatic damaged due to aloxan induction. On the other hand, chlorpropamide, K-carrageenan, and i-carrageenan were able to increase the size of Langerhans islet because they can support the recovery process of pancreatic organ damaged due to aloxsan induction. Insulin injection was unable to increase the ratio of Langerhans islet diameter to the amount of B-cells. While, chlorpropamide, K-carrageenan, and i-carrageenan were able to increase the ratio of Langerhans islet diameter to the amount of ß-cells. The macroscopic observation revealed that there was a black spot on the rat intestine receiving carrageen.
Identifikasi, Uji Antioksidan, dan Uji Toksisitas Senyawa Bioaktif dalam Caulerpa sertularioides (Vahl.) C. Agard. Thamrin Wikanta; Swasono R. Tamat; Magdalena Sinta Maryati
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Caulerpa sertularioides (Vahl.) C. Agard. is a green seaweed species in the Caulerpaceae family which has been used for medication. Extraction of bioactive compounds from Caulerpa sertularioides have been carried out and screened based on the antioxidative property against DPPH and on toxicity test by the brine shrimp lethality test (BSLT) method. Phytochemical screening of the methanol extract indicated the presence of a triterpen. The methanol extract was partitioned in sequence with n-hexanewater (1:1) and chloroform-water (1:1). The IC50 value of the water extract was found 327 ug/ml and the LCs value was 115 ug/ml as compared to the ICs of vitamin C positive control of 21 ug/ml. Fractination of the water extract in a silica gel column with various ratio of chloroform-methanol did not produce distinct separation. Fractions were analyzed on TLC and fractions of similar components were collected. Fraction I has IC50 value of 326 ug/ml and LC50 value of 68 ug/ml. Further separation and purification of fraction I on a preparative thin layer chromatographic system using chloroform-methanol (50:1) produced five isolates bearing antioxidative activity. The third isolate that showed the strongest activity, when analyzed using a GC-MS system indicating seven compounds, but only [(2-fluorophenyl)methyl)-1H-purin-6-amine that may have an antioxidative activity.
Co-Authors Lestahari Rahayu Lina S. Maulina Magdalena Sinta Maryati Rahma Damayanti Swasono R. Tamat