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MOHAMAD ASPOLLAH SUKARI
UNIVERSITI PUTRA MALAYSIA
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Pengaruh Lupene-ol dan Lupene-on dari Aegle marmelos Correa terhadap Pelepasan Enzim β-hexoaminidase dari Sel Mast AGUNG ENDRO NUGROHO; SUGENG RIYANTO; MOHAMAD ASPOLLAH SUKARI; KAZUTAKA MAEYAMA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 1 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

20(29)-lupene-3α-ol and 20(29)-lupene-3-on are pentacyclic triterpenes isolated from Aegle marmelos Correa collected in Yogyakarta, Indonesia. Their molecular structures were confirmed in Universiti Putra Malaysia. These compounds were obtained from petroleum ether extract of the plant’s stem bark. This study investigated the effects of the compounds on the β -hexoaminidase enzyme release from mast cell culture (RBL-2H3 cell line). DNP24-BSA was used as immunologic inducer for the β -hexoaminidase release from the mast cells. The enzyme release was determined by colorimetric methods with a substrate, p-nytrophenyl-2-acetamydo-2-deoxy- β -D-gluko-pyranocide, and a microplate reader at 405 nm. lt was found that treatment with 20 µg/ml DNP24-B SA could stimulate the release of β -hexoaminidase from RBL-ZH3 cells by 3O.83 ± 1.10%. Lupene-ol and lupene-on showed inhibitory effects on the β -hexoaminidase release from RBL-2H3 cells induced by DNP24-BSA in dose-dependent manner. At the dose of 100 µM, lupene-ol and lupene-on inhibited the β -hexoaminidaserelease by 35.69i4.7 5% and 39.19 9.56%, respectively. The ICN values of their effects on DNP24-BSA experiments were 59.40 µM and 72.51 µM. The results suggested that the inhibitory effect of lupene-ol and lupene-on on the β-hexoaminidase release involved mechanisms related to the interaction of IgE on the RBL-2H3 cells surface or intracellular signal transductions found in the mast cell degranulation. Key words: Aegle marmelos Correa, lupene-ol, lupene-on, mast cells, β -hexoaminidase enzyme.
Antagonisme Marmin terhadap Reseptor 5-Hidroksi Triptamin dan Reseptor Histamin pada Otot Polos Ileum Marmut AGUNG ENDRO NUGROHO; PUGU NOVI ARSITO; YANCE ANAS; JOKO TRI WIBOWO; SUGENG RIYANTO; MOHAMAD ASPOLLAH SUKARI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 2 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The exploration of several active compounds of medicinal plants Aegle marmelos Correa (Rutaceae) have found marmin that strongly inhibit the histamine release from mast cells. The present study investigated the antagonism phenomena of marmin on 5-hydroxy triptamine and histamine receptors in guinea-pig ileum smooth muscle. The study was performed using isolated-organ technique in Tyrode buffer medium. The agonists used to sensitize the receptors were histamine and 5-hydroxy triptamine. The type of antagonism was identified by considering: 1). the decrease of pD2 or Emax values of agonists due to pretreatment of marmin, and 2) Schild regression method. In the study, marmin (10 and 100 mM) decreased the maximum contraction effect of 5-HT by 40% and 60%, respectively. However, marmin did not influence the pD2 value of 5-HT . On the other side, marmin did not influence the maximum contraction effect of histamine, however, decreased the pD2 value of histamine. Based on Schild regression method, the pA2 value of marmin was 0.8016. In conclusion, marmin non-competitively antagonized 5-HT 4 receptor, and competitively antagonized histamine receptor in ileum smooth muscle.
Co-Authors Agung Endro Nugroho JOKO TRI WIBOWO KAZUTAKA MAEYAMA PUGU NOVI ARSITO SUGENG RIYANTO Yance Anas