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All Journal AGROTEKBIS AGROLAND: The Agricultural Sciences Journal J-PEN Borneo : Jurnal Ilmu Pertanian
Nirwan Sahiri
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences

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INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO Yulianti Rasud; Zainuddin Basri; Nirwan Sahiri
J-PEN Borneo : Jurnal Ilmu Pertanian Vol 2, No 2 (2019)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS BORNEO TARAKAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/jpen.v2i3.1533

Abstract

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D.  In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D.  Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci :  Induksi Kalus, Cengkeh, 2,4-D.
THE IDENTIFICATION OF MANGO MORPHOLOGY AND ANATOMY (MANGIFERAINDICA L) AT WEST TOBOLI AND POMBALOWO VILLAGE, PARIGI MOUTONG. Widya Tri Wulandari; Nirwan Sahiri; Enny Adelina
AGROLAND: The Agricultural Sciences Journal Vol 5, No 1 (2018)
Publisher : Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.482 KB)

Abstract

Mango (Mangiferaindica L.) is a type of tropical fruit  which is favored by people in the world and becomes a commodity trade between countries. According to the information, known as mango The Best Loved-Tropical, accompany the popularity of durian as the King of Fruit. The main product of the mango is its thatis  usuallyconsumed in fresh or processed products. But in addition to the fruit,,other components that also played an important role were the mangoes leaves than can be benefit from medicinal plants as an alternative. This research aimed to obtain a diversity of mango tree based on morphological and anatomical analysis of plant the mango in the West Toboli village and Pombalowo Regency, Parigi Moutong Regency. This research was carried out in the village of West Toboli,  District Toboli Parigi North and the village of Pombalowo sub-district of Parigi, Parigiranga Reddy District and Laboratory Science (FMIPA), the University of Tadulako conducted in July to August 2016. This research method using exploratory, as for a character that observed was the height of the plant (m), diameter (cm), the shape of the canopy, the long strands of leaves (cm) width (cm) leaf blade, petiole length (cm). Cluster analysis results in the village there were three groups of Western Toboli diverse namely TBB12, TBB14 and TBB11, in the village of Pombalowo there were three diverse groups namely PBL1, PBL5 and PBL8. Whereas in the second the village obtained a combined five diverse groups namely TBB12, TBB14, TBB2, PBL1 and TBB11. Characters that distinguished the selected accessions were the height of plants, and the diameter of the rod.
Co-Authors Abdul Syakur Adrianton A. Aidin, Anita Andi Ete Enny Adelina Iban, Iban Ichwan Madauna Murtando, Hefip Mutmainna, Mutmainna Ramdan Anata Septian Palma Ariany Weni Listyaningsih Widya Tri Wulandari Yulianti Rasud Zainuddin Basri