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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology
S Said
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Agriculture, Biological Sciences & Forestry

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Kelahiran Anak Sapi Hasil Fertilisasi secara in Vitro dengan Sperma E M Kaiin; S Said; B Tappa
Media Peternakan Vol. 31 No. 1 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.135 KB)

Abstract

The aim of this study was to know the viability of embryo after fertilized in vitro with frozen separated sperm (sexing sperm). Eleven frozen embryos were transferred to six Bali cow and five FH cow recipients. Two Bali cow recipients were positively pregnant but only one born as male calf which was appropriate with sex of straw being inseminated (Y sperm). One FH cow recipient was also pregnant but aborted in 2.5 months of pregnancy. It was concluded that embryo resulted from in vitro fertilization (IVF) with sexing sperm could grow and develop to be a calf which was appropriate with the sex of straw inseminated. Key words: sperm sexing, IVF, embryo transfer, Bali cow
Tingkat Fertilisasi dan Perkembangan Embrio Mencit yang Diberi Ekstrak Buah Merah S Said; O P Astirin; S Wahyuningsih
Media Peternakan Vol. 34 No. 2 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (862.383 KB) | DOI: 10.5398/medpet.2011.34.2.112

Abstract

In the present study examined the influence of red fruit extract (RFE) on fertilization rate and development of mice embryos during the pre-implantation stage. The application of RFE dose 0 ml/head/day, 0.05 ml/head/day, and 0.1 ml/head/day for seven days were conducted to examine its influence at the level of fertilization, then the application of RFE was continued during 0-3 days stage of pregnancy (0 day of pregnancy is the formation of the vaginal plug). The super-ovulation of the white mice was done by injecting of 5 IU PMSG and hCG (48 hours after PMSG), folowed by mating the females with males with ratio 1:1. The single-cell-embryos were collected 20 hours after the injection of hCG. The eggs were then fixed and stained before examinating the normal fertilization of eggs (formation 2 pronucleus). The morula and blastocysts were collected 84 hours after injection of hCG. The influence of the RFE with dose 0.1 ml on normal fertilization rate (formation 2 PN), significantly higher (46.01%) compared with dose 0.05 ml (37.51%) and controlled (16.38%). However, the influence of RFE with dose of 0.05 ml and 0.1 ml on the growth of pre-implantation stage embryos may inhibit the growth of mice embryos during the pre-implantation stage in vivo.
Co-Authors B Tappa E M Kaiin O P Astirin S Wahyuningsih