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All Journal Scripta Biologica
Sorta Basar Ida Simanjutak
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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AKTIVITAS GLUTATION PEROKSIDASE DAN KADAR GULA DARAH TIKUS DIABETES YANG DIBERI EKSTRAK DAUN KAPULAGA (Amomum cardamomum) Hafsah Riyanti; Sorta Basar Ida Simanjutak; Hery Winarsi
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.999 KB) | DOI: 10.20884/1.sb.2014.1.2.442

Abstract

Oxidative stress in a patient with diabetes known to decrease the activity of GSH-PX and increase the level of blood glucose. Glibenclamide is commonly used by patients with diabetes to lower the blood glucose. However, the long-term use of glibenclamide may induce the damage of pancreatic β-cells. The extract containing flavonoids and vitamin C of cardamoms leaf can reduce free radicals. The effect of cardamoms leaf extract on the enzymes activity and the level of blood glucose in diabetic rats is yet unknown. The aim of this study was to examine the effect of cardamoms leaf extract administration in the GSH-PX activities and the level of blood glucose of diabetic rats. The method used in this study was experimental with completely randomized design (CRD). There were three groups with five replicates for each. The first group, diabetic rats as control group were given no treatment; the second group, diabetic rats were given cardamoms leaf extract at the dose of 100 mg/kg of body weight; the third group, the diabetic rat were given glibenclamide at the dose of 2 mg/kg of body weight. Blood samples were taken three times at 0, 7, and 14 days after each treatment. Parameters measured were GSH-PX activity and the level of blood glucose. The data were analyzed using ANOVA F-test with a confidence level of 95%. The result of this study showed that the cardamoms leaf extract treatment decreased the activity of GSH-PX activity and decreased the level of blood glucose at a significant level (p < 0.05) from 278.8 to 101.4 mg/dl.
DIFERENSIAL LEUKOSIT IKAN GURAMI (Osphronemus gouramy Lac.) DENGAN PERBEDAAN LEVEL SUPLEMENTASI Spirulina platensis DALAM PAKAN Hikmah Widyaningrum; Sorta Basar Ida Simanjutak; Priyo Susatyo
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (710.971 KB) | DOI: 10.20884/1.sb.2017.4.1.383

Abstract

The aim of this research was to know the effect of supplemented Spirulina platensis in diet on leukocytes differential of gourami (Osphronemous gouramy Lac.) and to obtain the best dose. This research was carried out experimentally using a completely randomized design (RAL), which consists of 4 treatments and 3 repetitions. The fish fed with diets which contain different levels of (0, 2.4, and 6g/kg) Spirulina platensis were investigated. Sampling was done 4 times at intervals of 2 weeks of the feeding experiment, leukocyte differential (monocyte, lymphocyte, neutrophil, eosinophyl) of blood taken from fish were determined. The results showed that the highest percentage of lymphocytes was found in level of supplementation of 4 g/kg of diets, whereas the highest percentage of monocytes, neutrophils and eosinophils were found at the level suplemetation 2 g/kg of diets. Level supplementation of S. platensis 4 g/kg of diets is an efficient and effective level in stimulating immune system cells of gourami, particularly lymphocytes cell.
Co-Authors Hafsah Riyanti Hery Winarsi Hikmah Widyaningrum Priyo Susatyo