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Hendro Pramono
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IDENTIFIKASI BAKTERI PENGOKSIDASI BESI DAN SULFUR BERDASARKAN GEN 16S rRNA DARI LAHAN TAMBANG TIMAH DI BELITUNG Dhewanti Puspitasari; Hendro Pramono; Oedjijono Oedjijono
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.765 KB) | DOI: 10.20884/1.sb.2014.1.1.12

Abstract

Heavy metals contamination disturb balance and diversity of microorganism in soil. Microorganisms which can able to survive in those conditions are bacteria capable of oxidizing heavy metals. Identification based on 16S rRNA was used to determine characteristics and phylogenetic relationship of bacteria which can oxidize iron and sulphur in tin mining areas. The aim of this research was able to determine the bacterias characteristics isolated from tin mining areas and determine the phylogenetic relation of iron-sulphur oxidizing bacteria on tin mining soil in Belitung based on 16S rRNA sequences. This research was done using descriptive method, including isolation, morphological characterization, and identification based on 16S rRNA sequences. Morphology characterization includes colony and cell morphology through Gram staining. Molecular characterization includes amplification of 16S rRNA gene (Polymerase Chain Reaction/ PCR), electrophoresis amplicon and sequencing. Bacteria identification was done by comparing the 16S rRNA gene sequence in GenBank. The result showed three bacterias were identified by 16S rRNA have a similarity with Bacillus anthracis strain Ames, Bacillus cereus ATCC 14579, Staphylococcus sciuri subsp. Sciuri strains DSM 20345 and Micrococcus luteus NCTC 2665.
DETEKSI KERAGAMAN SPESIES BAKTERI METANOGEN RUMEN SAPI MENGGUNAKAN KLONING GEN 16S rRNA DAN SEKUENSING Shoffiana Noor; Hendro Pramono; Saefuddin Aziz
Scripta Biologica Vol 1, No 4 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.925 KB) | DOI: 10.20884/1.sb.2014.1.4.43

Abstract

Ruminants produce methane gas which contributes to enhanced greenhouse effect in the atmosphere. Cattle issued the highest methane during the fermentation of feed in the rumen. Methane gas produced by methanogen bacteria in carbohydrates anaerobic fermentation. Methanogen bacteria are difficult to obtain diversity information because difficult cultured. One technique can be used is molecular rRNA 16S gene cloning and sequencing. This study was aims to determine the species diversity of methanogen bacteria in cattle's rumen using rRNA 16S gene cloning and sequencing technique by survey method. The results obtained 51 clones with 800 bp insert size length. The sequencing resulted 2 different sequences, ie 8-3L21 clone bacterium uncultured and BBS-12 clone methanogens rumen uncultured rRNA 16S gene partial sequence with 99% and 100% similarity. The Genus sequences for gene 1 and 3 were Prevotella (24%), Clostridium (1.5%), and other uncultured bacteria, whereas the 2 gene sequences of species was Methanobrevibacter ruminantium (21.83%), M. millerae (29.17%), M. gottschalkii (6.47%), Methanosphaera stadtmanae, and Methanobacterium alcaliphilum. This research provides scientific information about cattle rumen methanogen bacteria species diversity which can be used as a basis for control of cattle rumen methanogen bacteria. 
Co-Authors Dhewanti Puspitasari Oedjijono Oedjijono Saefuddin Aziz Shoffiana Noor