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Oedjijono Oedjijono

Fakultas Biologi, Universitas Jenderal Soedirman

Author-ID : 3698384

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IDENTIFIKASI BAKTERI PENGOKSIDASI BESI DAN SULFUR BERDASARKAN GEN 16S rRNA DARI LAHAN TAMBANG TIMAH DI BELITUNG Dhewanti Puspitasari; Hendro Pramono; Oedjijono Oedjijono
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Heavy metals contamination disturb balance and diversity of microorganism in soil. Microorganisms which can able to survive in those conditions are bacteria capable of oxidizing heavy metals. Identification based on 16S rRNA was used to determine characteristics and phylogenetic relationship of bacteria which can oxidize iron and sulphur in tin mining areas. The aim of this research was able to determine the bacterias characteristics isolated from tin mining areas and determine the phylogenetic relation of iron-sulphur oxidizing bacteria on tin mining soil in Belitung based on 16S rRNA sequences. This research was done using descriptive method, including isolation, morphological characterization, and identification based on 16S rRNA sequences. Morphology characterization includes colony and cell morphology through Gram staining. Molecular characterization includes amplification of 16S rRNA gene (Polymerase Chain Reaction/ PCR), electrophoresis amplicon and sequencing. Bacteria identification was done by comparing the 16S rRNA gene sequence in GenBank. The result showed three bacterias were identified by 16S rRNA have a similarity with Bacillus anthracis strain Ames, Bacillus cereus ATCC 14579, Staphylococcus sciuri subsp. Sciuri strains DSM 20345 and Micrococcus luteus NCTC 2665.

ANTIBACTERIAL CAPACITY OF Streptomyces ISOLATE FROM A MANGROVE PLANT RHIZOSPHERE Avicennia marina Rendy Setya Wardana; Dini Ryandini; Oedjijono Oedjijono
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

This research was conducted to obtain Streptomyces isolates from Avicennia marina rhizosphere capable of inhibiting E. coli and S. aureus growth, to investigate the capability and the characteristics of its antibacterial compound. This study completed the isolation by applying pour plate method on SCN agar medium. Antagonistic screening and selection processes were carried out by diffusion and dilution methods. Observation on the characteristic of the antibacterial compound applied was TLC method and MIC assay. This research confirmed the antibacterial compound capability by applying bioautography assay. Parameters measured consisted of inhibition zone diameter, Rf value on a bioautography plate, and the lowest concentration capable of inhibiting bacterial growth. Out of 16 isolates of Streptomyces obtained, Streptomyces 404 showed higher antagonistic activity than others. Inhibition zone diameter reached 20–25 mm in E. coli and S. aureus growth, respectively. TLC assay showed three spots in which two of them confirmed antibacterial activity in the bioautography assay that yielded Rf values of 0.47 for E. coli and 0.72 for S. aureus, while MIC assay showed that the lowest extract concentration inhibited bacterial growth was 20%.

EFEKTIFITAS BAKTERI ENDOFIT DAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DALAM MENINGKATKAN PERTUMBUHAN TANAMAN PADI Oryza sativa L. Aria Rizki Ramadhan; Oedjijono Oedjijono; Ratih Dewi Hastuti
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Rice plant needs essential and non-essential nutrients to grow. However, the use of inorganic fertilizers may affect the soil microbe community which expected to increase the availability of nutrients for both plants and microbes, a condition that could control several types of diseases. Many efforts were made to find alternative fertilizer which environmentally friendly, for instance by exploiting microorganisms such as endophytic bacteria associated with plant tissue or seed plant cell. This research aimed to determine the effect of endophytic bacteria, the concentration of IAA, and the combination of endophytic bacteria with a concentration of IAA, on the growth of rice plant. This research was conducted at the Laboratory of Soil Biology, The Soil Research Institute located in Cimanggu Bogor from December 2015 to February 2016. This research was a Completely Randomized Design (CRD) with two factors, i.e., the inoculant of endophytic bacteria (2.2 KT, KR 6, and I CM), and the concentration of IAA (0, 0.01, 0.1, 0.5, and 1 ppm). Three replicates were applied for each treatment of the inoculant combined with different doses of IAA. The variety of rice plants tested were Inpari 13 and IR 64. The potential test has been applied to determine the factor with the highest yield of the rice plant growth. The parameters observed were the height of the rice plant, the length of the rice plant roots, and the total weight of the plant including root. The results showed the inocula of endophytic bacteria alone had no effect to increase growth for both IR 64 and Inpari 13 rice variety. The IAA concentration of 0.1 ppm was able to increase the height of rice plant and the length of rice plant root for both IR 64 rice plant and Inpari 13. The combined treatment of KR 6 bacterial inoculum with 1 ppm of IAA concentration was effectively promoted rice plant growth for both IR 64 and Inpari 13.

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