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All Journal Berkala Ilmiah Kedokteran Duta Wacana
Ardiyan, Yustina Nuke
Bagian Histologi, Fakultas Kedokteran, Universitas Kristen Duta Wacana, Jalan Dr. Wahidin Sudirohusodo Nomor 5-25, Yogyakarta, Daerah Istimewa Yogyakarta 55224
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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A REVIEW OF QUALITY CONTROL IN HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY STAINING Yustina Nuke Ardiyan
Berkala Ilmiah Kedokteran Duta Wacana Vol 5, No 2 (2020): BERKALA ILMIAH KEDOKTERAN DUTA WACANA
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/bikdw.v5i2.180

Abstract

Background: Tissue staining methods need controls to give best quality results. Controls is necessary to support the validity, correctness, and reliability of the staining results. Haematoxylin and Eosin (HE) and immunohistochemistry (IHC) staining require different control quality. Objective: To discuss the utilization of controls used in tissue staining. Method: This is a narrative review with literature from 2008-2016. Results: Routine staining usually does not require any control. Specific stainings require controls to support the validity, correctness, and reliability of the staining results. Histochemistry staining requires only positive control, while positive and negative controls must be included in IHC staining. A tissue section which expresses the protein of interest is reffered to positive control, whereas those without any target antigen expression are defined as negative control. Conclusion: Histochemistry and IHC staining controls are needed to obtain the validity of the tissue or cell samples.
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