Martha Sari
Pusat Penelitian Bioteknologi LIPI, Cibinong

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PENINGKATKAN KUALITAS MINYAK GORENG CURAH MENGGUNAKAN ADSORBEN LEMPUNG DESA GEMA TERAKTIVASI Sophia, Halida; Muchtar, Akmal; Sari, Martha
Sistem Informasi Vol 5 No 1 (2014): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.302 KB)

Abstract

Telah dilakukan penelitian tentang peningkatan mutu minyak goreng curah menggunakan adsorben lempung asal Desa Gema, Kabupaten Kampar, Provinsi Riau. Penelitian ini meliputi: (1) Pembuatan adsorben dengan aktivasi menggunakan H2SO4 0,5 M, selama 3 jam dengan kecepatan pengadukan 500 rpm (2) Pemurnian minyak goreng secara adsorbsi dan mengetahui mutu minyak curah setelah adsorbsi berupa bilangan peroksida, bilangan asam, kandungan air, warna. Proses adsorpsi diamati dengan variasi massa (1, 3, 5, dan 7 gram) dengan waktu kontak 30 menit. Semua nilai yang diperoleh dibandingkan dengan SNI 3741:2013. Hasil yang didapat menunjukkan lempung teraktivasi H2SO4 mampu menurunkan bilangan peroksida, bilangan asam, dan kandungan air secara maksimal masing-masing sebesar 31,02%, 36,36%, dan 38,89% dengan 7 gram lempung teraktivasi.
STRUCTURAL CHARACTERIZATION OF FRACTIONATED GLYCOLIPID BIOSURFACTANTS SYNTHESIZED BY Pseudozyma aphidis YB205 Sari, Martha; Kartika, I Made; Kusharyoto, Wien
Teknologi Indonesia Vol 39, No 3 (2016)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v39i3.278

Abstract

Fractionation and structural characterization of glycolipid biosurfactant produced by yeast Pseudozyma aphidis strain YB205 was conducted. The yeast strain was grown in a nutrient broth with crude oil as the carbon sources and the glycolipid biosurfactant produced was isolated. The crude glycolipid was fractionated using column chromatography followed by complete separation and purification using extraction technique employing  different organic solvents. The fractions were subjected to activity test using oil displacement assay followed by chemical identity test using thin layer chromatography. In order to elucidate its chemical structure, the most active fraction was subjected NMR and FTIR analysis. Results showed that six major fractions were generated all of which showed biosurfactant activity. Four fractions is fractions 2, 4, 5, and 6 showed glycolipid characteristics and fraction 6 showed the highest biosurfactant activity. Combination of NMR and FTIR spectroscopy spectra indicated that chemical structures of fraction 6 belonged to glycolipid species.