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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Prapti Sedijani
Program Studi Pend. Biologi Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Explorasi Mikroba Beraroma dari Bahan Alam Prapti Sedijani; Dewa Ayu Citra Rasmi; Syamsul Bahri
Jurnal Penelitian Pendidikan IPA Vol. 5 No. 2 (2019): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v5i2.222

Abstract

Abstract: The Scented volatile compound has a huge market as its wide applications in daily life. The extraction of this compound from natural resources produces a very low rendement. Microbes synthesize secondary metabolit might resemble of their host have been explored from scented natural resources (flowers: Damask rose, Jasminum sambac and Plumeria acutifolia); cymbopogon, muntinga calabura L and leave of Panadus amaryllis).  It was found 12 bacterial and 14 fungal scented isolates that were various in terms of scent types and intensities. They were bread starters like the smell, alcohol like the smell, sweet sugar like the smell, salaka like the smell and fresh sugarcane juice like smell. Several isolates emit original host like the smell in very low intensities. Persistency scent test showed that the scents were persistently emitted when cultured on PDA medium.  Key Words: Scented volatile; Damask rose; microba; exsplorasi
Powerful Lipolytic Activity of Fungi Isolated from Coconut and Avocado Flesh on Different pH and Temperature Prapti Sedijani; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati; Riska Agustina Anggriani
Jurnal Penelitian Pendidikan IPA Vol. 7 No. SpecialIssue (2021): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v7iSpecialIssue.1261

Abstract

Lipase is widely applied in various fields of industries elevating its economic value. The demand for lipase keeps increasing open up working opportunities. Isolates showing lipolytic activity obtained previously are tested for their activity on different pH mediums: 7, 8, 9, and 10; and at different temperatures. The fungi are grown on SDA medium supplemented with Olive Oil, emulsifier, Tween 80, and Rhodamine B. The experiment was made in 3 replicates and is incubated at room temperature of 30oC. Lipase activity was calculated based on the clear zone around colonies observed on day 2 and day 3 after inoculation. The result shows that those isolates are highly active on various pH at RT, and the activity slightly reduces at 30oC. This result suggests that a wide range of their applications are at room temperature when pH is a limiting factor for the applications
Co-Authors Dewa Ayu Citra Rasmi Dewa Ayu Citra Rasmi Kusmiyati Kusmiyati Riska Agustina Anggriani Syamsul Bahri