Arief Widyantoro
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret

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Antagonisme Bacillus terhadap Infeksi Layu Fusarium pada Bibit Pisang Hasil Kultur Jaringan Hadiwiyono Hadiwiyono; Arief Widyantoro; Salim Widono
Agrosains : Jurnal Penelitian Agronomi Vol 15, No 1 (2013): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.832 KB) | DOI: 10.20961/agsjpa.v15i1.18990

Abstract

Fusarium wilt (Fusarium oxysporum f.sp. cubense) is an important disease in banana. Fusarium wilt was hard to control because the pathogen can survive in many kind of soils type although there is no host. Therefore, overcoming the disease is urgently needed such as biological control. The endophytic Bacillus of banana was begun to use as antagonist agent to the pathogen. This research aimed to study the mechanism of antagonism and physiological character of Bacillus. There were 27 Bacillus isolates examined in-vitro to test the production of IAA, HCN, chitinase, pectinase, and antagonism. The top ten isolates based on the test in vitro were used to test in planta. The research showed that Bacillus were able to produce IAA, HCN, chitinase, pectinase, and able to retard the growth of Foc colony. The application of isolat B25 on banana seedling could decrease the disease intensity but still unable to prevent the fusarium wilt infection.