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All Journal International Journal of Biosciences and Biotechnology Agrikultura AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrosains: Jurnal Penelitian Agronomi PRIMA: Journal of Community Empowering and Services Journal of Biodiversity and Biotechnology Biodiversitas Journal of Biological Diversity Nusantara Bioscience

. Hadiwiyono

Department Of Agriculture Science, Graduated School Of Sebelas Maret University

Author-ID : 272484

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Other

Published : 16 Documents Claim Missing Document

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MORPHOLOGY, PHYSIOLOGY AND MOLECULAR CHARACTERISTICS OF OIL PALM (Elaeis guineensis Jacq.) ENDOPHYTIC Bacillus sp. Fifi Puspita; Hadiwiyono Hadiwiyono; Susilo Hambeg Poromarto; Dewi Indriyani Roslim
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Endophytic bacteria are the bacteria that live in plant tissues. In oil palm tissue there are many types of endophytic bacteria and have a role that can be beneficial for the plant, one of them is endophytic Bacillus sp. The aim of these research was to obtain morphology, physiology and molecular characteristics of endophytic Bacillus sp. originating from oil palm tissue. Sampling was done by random simple sampling method. Isolation of bacteria was performed on plant tissues such as roots, midribs, stems and leaves of oil palm plants. The results of morphological characterization such as colony color, colony shape and colony edge show similarity in each isolate but there are differences in the surface morphology of the colony, where there are 6 isolates with convex surface and 6 isolates with flat shape. Physiological test results such as catalase test, oxidase test, starch hydrolysis test, motility test and temperature effect test on bacterial growth showed positive results in each isolate. Molecular characterization using 16S rRNA primers based on BLASTn shows that all isolates tested have similarities with Bacillus sp. Based on the phylogenetic tree it was found that the endophytic bacteria of Ba-B2 isolates were associated with Bacillus flexus with 100% consistency index grouped at a distance of 0.03 and Ba-P2 isolates were related to Bacillus substilis at a distance of 0.01 with an 89% consistency index.

Quorum Sensing: Suatu Sistem Komunikasi Bakteri Fitopatogen, Peranannya Pada Proses Infeksi, dan Peluangnya sebagai Basis Pengembangan Strategi Baru dalam Pengendalian Penyakit Tumbuhan Hadiwiyono Hadiwiyono
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11765

Previously, it is thought that bacterium is an autonomous unicellular organism with no capacity for collective behavior. Now the paradigm has changed since there are communication between intercell bacteria. The phytopathogenic bacteria are able to regulate gene expression which play an important role in infection process in the host plant. The gene expression regulation involves an intercell communication in the population and this is called as quorum sensing (QS). This paper reviews the QS as a communication system of phythopathogenic bacteria, its role in infection process on the host plant, and its opportunity as the basis of novel strategy development on plant disease control.

Tanah Supresif: Terminologi, Sejarah, Karakteristik, dan Mekanisme Hadiwiyono Hadiwiyono
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11881

Many definitions of the suppressive soil purposed were proposed by some authors, however in summary, suppressive soil is the soil where virulent pathogen and susceptible host exist but the population and or disease produced by the pathogen are limited, due to the biotic factors supported by some specific environmental conditions. The suppressive soil phenomena has been recognized since 100 years ago, but it was thought as an interesting field since 30 years ago, and since the year many research topics related to the suppressive soil are conducted and leading to be popular. Based on the characteristic, suppressive soil could be grouped into general suppressive involving an antagonistic microbe complex being nontransferable, and specific suppressive caused by one or some antagonistic microbe being transferable. Antagonism mechanism of the microbes involved in the suppressiveness could be through antibiosis, competision, parasitism, predatism, and induced resistance.

EFFECT OF ALTITUDE AND WOUNDING ON BLOOD DISEASE PROGRESS OF PLANTAIN Hadiwiyono, S. Subandiyah, C. Sumardiyono, J. Widada, and M. Fegan.
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 2 (2007): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Effect of Altitude and Wounding on Blood Disease Progress of Plantain. In the latest decade, the blood disease of banana has spread in almost all provinces in Indonesia and caused wilting of millions banana clusters in several provinces. It is very difficult to control the disease due to the base data about ecology and epidemiology of the pathogen are still poorly understood. This research aimed to evaluate the effect of wounding of inoculation site on blood disease progress of plantain. The experiment was arranged using randomized completely block design It was conducted at three locations with altitude of 100, 1000, and 1600 m above sea levels as replication block. The treatments were wounding, unwounding inoculation site, inoculation, and uninoculation of plantain cv. Kepok Kuning Wounding was applied by stabbing with an injection pin around the corm of 15 stabs/seedling. The seedlings were planted singly in one liter of non sterile soil in plastic bag. Each treatment consisted of 5 seedlings which was replicated 3 times. Inoculation was done by soil drenching of 20 ml bacterial suspension at concentration of 108 cfu/ml two week after planting. The pathogen used for inoculation originated from low land area (about 100 m above sea level). Observation was done weekly for 5 weeks. The variables observed were wilt intensity and area under disease progress (AUDPC). The results showed that blood disease was able to establish at altitude of 1600 m above sea level. The disease progress however was slower that those at 100 and 1000 m above sea level. On wounded seedling, the disease progress was more aggressive than those on unwounded one.

EFEKTIVITAS CAISIN SEBAGAI TANAMAN PERANGKAP PATOGEN UNTUK PENGENDALIAN PENYAKIT AKAR GADA PADA KUBIS . Hadiwiyono; . Sholahuddin; Endang Sulastri
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

he research was conducted by observation on 5 plot kinds of cabbage planting. The plots consist of control plot with no planting Chinese cabbage, plot with planting Chinese cabbage as rotation plant on 38 day before planting of cabbage followed by manual eradication, plot with planting Chinese cabbage as rotation plant on 38 day followed by flooding for 14 days and soil tillage, plot with mixcropping Chinese cabbage on the early growth stage of the cabbage, and plot with intercropping of Chinese cabbage on 14 days before planting the cabbage. The results showed that planting Chinese cabbage as trap crop of the pathogen followed by eradication of the infected Chinese cabbage effectively controlled clubroot intensity and significantly restored the partial yields of the cabbage.

Kompatibilitas dan Efektivitas Azospirillum dan Streptomyces untuk Mengendalikan Penyakit Moler pada Bawang Merah di Alfisol Jumantono Hadiwiyono, Hadiwiyono; Fitriana, Faedah; Poromarto, Susilo Hambeg; Cahyani, Vita Ratri
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.50413

Pengaruh Azospirillum sp. dan Streptomyces sp. secara terpisah sebagai perlakuan tunggal pada kegiatan budidaya tanaman telah banyak dilaporkan pada penelitian sebelumnya, tetapi sebagai perlakuan kombinasi sebagai agens hayati masih terbatas. Penelitian ini bertujuan untuk menganalisis kompatibilitas dan efektivitas Azospirillum sp. dan Streptomyces sp. untuk pengendalian penyakit moler yang disebabkan oleh Fusarium oxysporum f. sp. cepae (FOCe) dan pertumbuhan bawang merah di tanah Alfisol Jumantono. Penelitian terdiri dari uji in vitro (uji antagonisme dan uji kompatibilitas) dan uji in vivo (penanaman pada polybag di screen house). Uji in vitro dilakukan dengan metode dual culture sementara uji in vivo dilakukan dengan Rancangan Acak Kelompok Lengkap (RAKL) yang terdiri dari lima perlakuan dan lima ulangan. Perlakuan terdiri dari perlakuan tanpa agens pengendali hayati, aplikasi fungisida, Azospirillum sp., Streptomyces sp., dan kombinasi Azospirillum sp. dengan Streptomyces sp. semua perlakuan diinkulasikan dengan FOCe.Variabel yang diamati pada uji in vitro yaitu kompatibilitas dan penghambatan pertumbuhan koloni FOCe, sementara pada uji in vivo variabel yang diamati adalah penghambatan terhadap intensitase penyakit serta pengaruhnya terhadap pertumbuhan bawang merah. Hasil penelitian menunjukkan bahwa secara in vitro Azospirillum sp. dan Streptomyces sp. kompatibel satu sama lain. Streptomyces sp. menekan pertumbuhan FOCe sebesar 52.96% lebih baik daripada Azospirillum sp. Akan tetapi, hasil uji in vivo menunjukkan Azospirillum sp. dan Streptomyces sp., baik secara individu maupun bersama-sama, belum berhasil memberikan pengendalian yang optimal terhadap penyakit moler pada bawang merah yang ditanam di tanah Alfisols Jumantono. Meskipun aplikasinya mampu mengurangi intensitas penyakit moler, tingkat efektivitasnya termasuk dalam kategori kurang. Selain itu, dampaknya terhadap parameter pertumbuhan bawang merah juga belum hasil yang diharapkan. Oleh karena itu, diperlukan peningkatan dalam metode aplikasi agens pengendali hayati guna mencapai hasil yang diinginkan.

Field Response to Pustule Bacteria and Yield of Several Soybean Varieties in the Mahogany Agroforestry System Under El Niño Dry Season in 2023 Haqiki, Zahra Tazkiyatun; Hadiwiyono, Hadiwiyono; Poromarto, Susilo Hambeg
AGRIVITA Journal of Agricultural Science Vol 47, No 2 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i2.4430

The factors that affect the low national soybean production are the reduction of agricultural land, the dry season, and extreme temperatures due to El Niño. Agroforestry can be one of the solutions, but the environmental conditions that make soybean plant growth less suitable can make it vulnerable to pathogens causing disease, such as a bacterial pustule caused by Xanthomonas axonopodis pv. glycines. This research aimed to evaluate the field response to bacterial pustules and the yield potential of various soybean varieties in mahogany agroforestry systems under the stress of the 2023 El Niño drought. This research was carried out during the El Niño dry season, August-October 2023, in the Alas Bromo Special Purpose Forest Area, Karanganyar District, Karanganyar Regency, Central Java. The research design used in this research was a complete randomized design with the factor of soybean varieties using six varieties planted under mahogany tree stands. The observed variables were height, the number of leaves, disease incidence, disease severity, infection rate, and area under the disease progression curve (AUDPC). The result showed that two varieties resistant to bacterial pustule: Agromulyo and Denasa-2. El Niño, dry season stress, causes low yield productivity; the highest yield was just 0.6 t/ha, produced by the Argomulyo variety.

Pemanfaatan Minyak Serai Sebagai Bahan Aktif Nanovirusida untuk Pengendalian Penyakit Kuning pada Cabai Septariani, Dwiwiyati Nurul; Hadiwiyono, Hadiwiyono; Harsono, Puji; Mawar, Munti'ah
PRIMA: Journal of Community Empowering and Services Vol 4, No 2 (2020): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v4i2.39797

Cabai merupakan komoditas yang penting secara ekonomi bagi Kelompok Tani Sidoluhur dan Kelompok Wanita Tani Puteri Petani Mandiri di Desa Cabeyan, Kecamatan Bendosari, Kabupaten Sukoharjo. Minimnya pengetahuan petani tentang pengendalian OPT menyebabkan pertanaman cabai terkendala berbagai penyakit terutama virus kuning yang dapat menurunkan hasil hingga 100%. Akhir-akhir ini berkembang formulasi nanoteknologi yang dapat meningkatkan efektivitas pestisida nabati. Nanoteknologi pestisida adalah formulasi partikel nanopestisida yang meningkatkan kelarutan, tingkat disolusi, dan dispersi saat aplikasi sehingga meningkatkan efikasi pestisida. Salah satu jenisnya yaitu nanovirusida yang berfungsi menekan infeksi virus pada tanaman. Kegiatan pengabdian ini bertujuan memperkenalkan nanovirusida nabati untuk mengatasi masalah penyakit pada pertanaman cabai. Metode kegiatan berupa penyuluhan mengenai cara mengendalikan berbagai organisme pengganggu tanaman (OPT) pada cabai, pembuatan demplot pembibitan, serta pelatihan teknis budidaya cabai dengan nanovirusida nabati. Aplikasi nanovirusida di lahan cabai lokasi mitra terbukti efektif menurunkan intensitas penyakit kuning lebih dari 50% dibandingkan tanpa pengendalian. Hasil kegiatan yang diperoleh berupa peningkatan keterampilan petani pada pengendalian virus kuning dan serangga penularnya dengan nanovirusida.

Evaluasi Efektivitas Mikoriza dan Pupuk Organik terhadap Pengendalian Serangan Fusarium oxysporum f.sp. Cepae pada Bawang Putih Supyani Supyani; Dwiwiyati Nurul Septariani; Zahra Tazkiyatun Haqiki; Hadiwiyono Hadiwiyono
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.71308

The main problem in garlic cultivation was root rot disease caused by Fusarium oxysporum f.sp. cepae. Environmentally friendly control following sustainable agriculture can be used to increase plant resistance by providing biological fertilizers such as mycorrhizae and organic fertilizers such as compost and husk charcoal. This research aimed to evaluate the effectiveness of the application of mycorrhizae and organic fertilizers against Fusarium oxysporum f.sp. cepae. The research was conducted from June to November 2021 at the Tawangmangu Horticultural Seed Garden in Jl. Raya Tawangmangu, Beji, Tawangmangu, Karanganyar. Laboratory test was conducted from December 2021 to January 2022 at Laboratory of Plant Pests and Diseases and Laboratory of Soil Chemistry, Faculty of Agriculture, Sebelas Maret University. The method used was completely randomized design with a combination of mycorrhizae treatment (2 gr plant -1), compost fertilizer (16 ton ha-1), and husk charcoal fertilizer (6 ton ha-1). The data obtained were analyzed using analysis of variance and then continued with the Duncan’s Multiple Range Test. The results showed that mycorrhizae gave 22.95% effectiveness in wilting control and 20.93% in tuber rot control. Organic fertilizers provide the wilting effectiveness control by 9.27% (compost) and 8.06% (husk charcoal), as well as tuber rot effectiveness control by 27.91% (compost) and 25.58% (husk charcoal). The combination of mycorrhizae and organic fertilizers gave the highest wilt control effectiveness of 26.07% in the combination of mycorrhizae, compost, and husk bran. The effectiveness of tuber rot control was found in the combination of Mycorrhizae and compost, which was 34.88%.

Effectiveness of Biofilmed Biofertilizer with Balanced Phosphate Fertilizer Dosage in Suppressing Purple Blotch Disease Intensity and Increasing Garlic Yield on Andisol Soil in Tawangmangu Mohammad Fikri Halim Madjid; Hadiwiyono Hadiwiyono; Sudadi Sudadi; Susilo Hamberg Poromarto
Journal of Biodiversity and Biotechnology Vol 5, No 2 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i2.114347

Garlic productivity in Indonesia is often constrained by purple blotch disease (Alternaria porri) . A sustainable control alternative involves utilizing biofilmed biofertilizer (BiO2) combined with phosphate (P) fertilizer . This study aimed to assess the effectiveness of balancing BiO2 and P dosage in suppressing purple blotch disease intensity and increasing garlic yield on Andisol soil in Tawangmangu . The research was conducted from May to September 2024 using a single-factor Completely Randomized Design (CRD) with four treatments: P0 (0% P + 100% NK + BiO2), P1 (50% P + 100% NK + BiO2), P2 (100% P + 100% NK + BiO2), and P3 (100% NPK without BiO2) . Data were analyzed using ANOVA followed by DMRT at a 95% significance level . Results indicated that combining BiO2 with P fertilizer was highly effective; it suppressed leaf spot disease intensity by 49.47% and increased garlic yields by 20.91% compared to the control . This confirms that integrating BiO2 with appropriate P fertilization is a viable strategy for improving garlic productivity in Andisol soil .

Co-Authors . Sholahuddin Agus Siswanto Andhika Wahyu Nugroho Argha Hyta Dimas Enggartiasto Arief Widyantoro Arief Widyantoro Bayu Rahmad Bernadip Budi Tjahjono Claudia Sandy Sofani Dedy Prasetyo Dedy Prasetyo Devi Puji Rahayu Devi Puji Rahayu DEWI INDRIYANI ROSLIM Dewi Indriyani Roslim Dwiwiyati Nurul Septariani Dwiwiyati Nurul Septariani Dwiwiyati Nurul Septariani, Dwiwiyati Nurul EDI PURWANTO Endang Sulastri Fifi Puspita FIFI PUSPITA Fitriana, Faedah Haqiki, Zahra Tazkiyatun Hardjono Sri Gutomo, Hardjono Sri I Gusti Bagus Wiksuana I Ketut Suada JAKA WIDADA Kumala Sari Lulu'il Maknun LUTFI TRI ANDRIANI MARK FEGAN Mawar, Munti'ah Mohammad Fikri Halim Madjid Natya Lakshita Poromarto, Susilo Hambeg Puji Harsono, Puji Salim Widono Salim Widono SITI SUBANDIYAH Subagiya Subagiya Sudadi Sudadi Sudadi Sudadi Sudadi Sudadi Supyani Supyani Supyani Supyani SUPYANI SUPYANI Supyani Supyani SUSILO HAMBEG POROMARTO SUSILO HAMBEG POROMORTO Susilo Hambeg Poromarto Susilo Hambeg Poromarto Susilo Hambeg Poromarto Susilo Hamberg Poromarto Vita Ratri Cahyani Vita Ratri Cahyani Zahra Tazkiyatun Haqiki

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