. Hadiwiyono
Faculty Of Agriculture Sebelas Maret University

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Succession of Fungal Community Structure in Degradative Land Caused by Basal Rot Pathogen of Garlic Cahyani, Vita Ratri; Rahayu, Devi Puji; Siswanto, Agus; Prasetyo, Dedy; Hadiwiyono, Hadiwiyono
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi 2014: Articles in Press (Vol. 11 No. 1)
Publisher : Faculty of Agriculture, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v0i0.216

Abstract

Land degradation causes a decreasement the ability of land in suppressing the development of pathogen FOCe that causes basal rot of garlic. In the garlic planting area in Tawangmangu discovered the fact that productive-suppressive land to FOCe the disease has low incidence (<1%) and degradative-conducive land with high incidens (≥60%). The research aims to study the succession of common fungal community structure and FOCe in the rhizosphere of garlic on both land for garlic plantation. The study was conducted from April to September 2013 in productive-suppressive land (Pancot) and degradative-conducive (Gondosuli) for soil sampling and laboratory analysis in Biologi Tanah UNS for fungi and FOCe analysis. The composite method used for sampling and the garlic rhizosphere samples  which is had healthy plants conditions aged 0, 20, 40, and 60 days after planting (DAP), and the garlic rhizosphere samples with healthy plants and diseased condition aged 80, 100, and 120 days after planting (DAP). Laboratory analysis using PDA culture medium as common fungi growing medium and SFA as a growing medium FOCe then observed population density and diversity. The method was pour plate method with 10-2 to 10-7 dilution. The results showed the population and diversity of fungi and FOCe have dynamics fluctuation. The fungi population in productive-suppressive land lower than degradative-conducive, with each value 108 and 3,5x108 CFU gram-1 soil. FOCe population on degradative-conducive land was higher than productive-suppressive land, and the highest population in both land at age 20 and 100 DAP with a FOCe density of each land about 1,25x107 and 1,66x107 CFU gram-1 soil. During the growth periode of garlic, the fungi diversity on productive-suppressive land always indicate higher than degradative-conducive land.
Daun Mimba, Sirih, dan Pahitan sebagai Fungisida Nabati: Efektifitas dan Kompatibelitasnya dengan Trichoderma untuk Pengendalian Penyakit Akar Gada (Plasmodiophora brassicae) Hadiwiyono, Hadiwiyono
Caraka Tani: Journal of Sustainable Agriculture Vol 20, No 2 (2005): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2368.34 KB) | DOI: 10.20961/carakatani.v20i2.20490

Abstract

The research aims to evaluate effectiveness of leaf extract oh neem, piper, and eupathorium as botanical pesticide and its compatibility which use of biological control agents Trichoderma for control of club root disease. The test conducted in greenhouse belong to Faculty of Agriculture, University of Sebelas Maret at Surakarta., on June till September 2004. The research used completely randomized design with 3 replications consisted 10 soil treatments before planting using leaf extract of neem (Azadirachta indica), Piper betle, and Eupathorium inulifolium and Trichoderma or its combinations. As dependent variables are disease severity, weight of health root, weight of fresh biomass and dryy biomass.The result of the research showed that soil treatment using leaf extract of neem, piper, and euphatorium could reduce effectively disease severity of club root. Treatment combinations of leaf extract of neem, piper, and euphatorium could increase effectiveness of control of club root and compatible with soil treatment using Trichoderma so it could increase effectiveness of the disease control.
Pengendalian Infeksi Akar Gada pada Pembibitan Kubis dengan Pupuk Hijau Daun Paitan Pratiwi, Diah Ayu; Gutomo, Hardjono Sri; Hadiwiyono, Hadiwiyono
Agrosains : Jurnal Penelitian Agronomi Vol 17, No 2 (2015): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.01 KB) | DOI: 10.20961/agsjpa.v17i2.18664

Abstract

Clubroot is a major disease that often infects cabbage caused by Plasmodiophora brassicae Wor.. The leaf of T. diversifolia is an example of weeds that can be used as green leaf manure. The research aimed to study the effectiveness and dosage of green manure from Tithonia leaf to control clubroot. In planta test was conducted by using factorial completely randomized design, the first factor was dosages of Tithonia green manure and second factor was application time of fertilizing. The research showed that green manure from Tithonia leaf effectively suppressed infection of P. brassicae and was capable of increasing growth variable of cabbage seedling. Fertilizing with green leaf manure of Tithonia with dosage of 10 ton ha-1 could decreased intensity of P. brassicae infection up to 10,18%. The application time of fertilizing 0 days after planting has the highest weight of cabbage seedling biomass is 14,10 g.
EFFECT OF ALTITUDE AND WOUNDING ON BLOOD DISEASE PROGRESS OF PLANTAIN Hadiwiyono, S. Subandiyah, C. Sumardiyono, J. Widada, and M. Fegan.
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 2 (2007): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.812 KB) | DOI: 10.23960/j.hptt.27111-116

Abstract

Effect of Altitude and Wounding on Blood Disease Progress of Plantain. In the latest decade, the blood disease of banana has spread in almost all provinces in Indonesia and caused wilting of millions banana clusters in several provinces. It is very difficult to control the disease due to the base data about ecology and epidemiology of the pathogen are still poorly understood. This research aimed to evaluate the effect of wounding of inoculation site on blood disease progress of plantain. The experiment was arranged using randomized completely block design It was conducted at three locations with altitude of 100, 1000, and 1600 m above sea levels as replication block. The treatments were wounding, unwounding inoculation site, inoculation, and uninoculation of plantain cv. Kepok Kuning Wounding was applied by stabbing with an injection pin around the corm of 15 stabs/seedling. The seedlings were planted singly in one liter of non sterile soil in plastic bag. Each treatment consisted of 5 seedlings which was replicated 3 times. Inoculation was done by soil drenching of 20 ml bacterial suspension at concentration of 108 cfu/ml two week after planting. The pathogen used for inoculation originated from low land area (about 100 m above sea level). Observation was done weekly for 5 weeks. The variables observed were wilt intensity and area under disease progress (AUDPC). The results showed that blood disease was able to establish at altitude of 1600 m above sea level. The disease progress however was slower that those at 100 and 1000 m above sea level. On wounded seedling, the disease progress was more aggressive than those on unwounded one.
Kompatibilitas dan Efektivitas Azospirillum dan Streptomyces untuk Mengendalikan Penyakit Moler pada Bawang Merah di Alfisol Jumantono Hadiwiyono, Hadiwiyono; Fitriana, Faedah; Poromarto, Susilo Hambeg; Cahyani, Vita Ratri
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.50413

Abstract

Pengaruh Azospirillum sp. dan Streptomyces sp. secara terpisah sebagai perlakuan tunggal pada kegiatan budidaya tanaman telah banyak dilaporkan pada penelitian sebelumnya, tetapi sebagai perlakuan kombinasi sebagai agens hayati masih terbatas. Penelitian ini bertujuan untuk menganalisis kompatibilitas dan efektivitas Azospirillum sp. dan Streptomyces sp. untuk pengendalian penyakit moler yang disebabkan oleh Fusarium oxysporum f. sp. cepae (FOCe) dan pertumbuhan bawang merah di tanah Alfisol Jumantono. Penelitian terdiri dari uji in vitro (uji antagonisme dan uji kompatibilitas) dan uji in vivo (penanaman pada polybag di screen house). Uji in vitro dilakukan dengan metode dual culture sementara uji in vivo dilakukan dengan Rancangan Acak Kelompok Lengkap (RAKL) yang terdiri dari lima perlakuan dan lima ulangan. Perlakuan terdiri dari perlakuan tanpa agens pengendali hayati, aplikasi fungisida, Azospirillum sp., Streptomyces sp., dan kombinasi Azospirillum sp. dengan Streptomyces sp. semua perlakuan diinkulasikan dengan FOCe.Variabel yang diamati pada uji in vitro yaitu kompatibilitas dan penghambatan pertumbuhan koloni FOCe, sementara pada uji in vivo variabel yang diamati adalah penghambatan terhadap intensitase penyakit serta pengaruhnya terhadap pertumbuhan bawang merah. Hasil penelitian menunjukkan bahwa secara in vitro Azospirillum sp. dan Streptomyces sp. kompatibel satu sama lain. Streptomyces sp. menekan pertumbuhan FOCe sebesar 52.96% lebih baik daripada Azospirillum sp. Akan tetapi, hasil uji in vivo menunjukkan Azospirillum sp. dan Streptomyces sp., baik secara individu maupun bersama-sama, belum berhasil memberikan pengendalian yang optimal terhadap penyakit moler pada bawang merah yang ditanam di tanah Alfisols Jumantono. Meskipun aplikasinya mampu mengurangi intensitas penyakit moler, tingkat efektivitasnya termasuk dalam kategori kurang. Selain itu, dampaknya terhadap parameter pertumbuhan bawang merah juga belum hasil yang diharapkan. Oleh karena itu, diperlukan peningkatan dalam metode aplikasi agens pengendali hayati guna mencapai hasil yang diinginkan.
Field Response to Pustule Bacteria and Yield of Several Soybean Varieties in the Mahogany Agroforestry System Under El Niño Dry Season in 2023 Haqiki, Zahra Tazkiyatun; Hadiwiyono, Hadiwiyono; Poromarto, Susilo Hambeg
AGRIVITA Journal of Agricultural Science Vol 47, No 2 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i2.4430

Abstract

The factors that affect the low national soybean production are the reduction of agricultural land, the dry season, and extreme temperatures due to El Niño. Agroforestry can be one of the solutions, but the environmental conditions that make soybean plant growth less suitable can make it vulnerable to pathogens causing disease, such as a bacterial pustule caused by Xanthomonas axonopodis pv. glycines. This research aimed to evaluate the field response to bacterial pustules and the yield potential of various soybean varieties in mahogany agroforestry systems under the stress of the 2023 El Niño drought. This research was carried out during the El Niño dry season, August-October 2023, in the Alas Bromo Special Purpose Forest Area, Karanganyar District, Karanganyar Regency, Central Java. The research design used in this research was a complete randomized design with the factor of soybean varieties using six varieties planted under mahogany tree stands. The observed variables were height, the number of leaves, disease incidence, disease severity, infection rate, and area under the disease progression curve (AUDPC). The result showed that two varieties resistant to bacterial pustule: Agromulyo and Denasa-2. El Niño, dry season stress, causes low yield productivity; the highest yield was just 0.6 t/ha, produced by the Argomulyo variety.
Identification of plant growth promoting rhizobacteria around Pulang Pisang Food Estate, Central Kalimantan, Indonesia Andriani, Lutfi Tri; Poromarto, Susilo Hambeg; Supyani, Supyani; Purwanto, Edi; Hadiwiyono, Hadiwiyono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225169-178

Abstract

Plant growth-promoting bacteria are well known as biostimulants, biofertilizers, bioprotectants, and biodegraders. The Pulang Pisau Food Estate is an Indonesian government program aimed at increasing rice crop production to achieve sustainable food self-sufficiency. Research on rhizobacteria in the Pulang Pisau Food Estate area is still relatively limited. In this study, we conducted in vitro assays to evaluate the potential of indigenous bacterial isolates from the Pulang Pisau Food Estate as plant growth-promoting rhizobacteria (PGPR). The study focused on four bacterial isolates, which were tested for plant growth-promoting traits including phosphate solubilization, indole-3-acetic acid (IAA) production, effects on rice seed germination, and detached leaf assays to assess the ability of rhizobacteria to inhibit bacterial pathogens. The results of 16S rRNA gene identification suggested that isolates UNS-P1, UNS-P3, and UNS-R1 were closely related to Bacillus cereus, while one previously identified isolate (UNS-R2) was confirmed as Bacillus subtilis. All bacterial strains were able to produce IAA, while only one isolate demonstrated the ability to solubilize phosphate. In the germination test, no significant differences were observed in root length, but a significant difference in shoot (plant) height was detected. Bacillus subtilis (UNS-R2), at a 10³ dilution, resulted in significantly greater plant height compared to other treatments. Among the four bacterial isolates, only one showed the ability to inhibit the pathogen Pantoea ananatis. These results suggest that indigenous bacterial isolates from the Pulang Pisau Food Estate have potential as plant growth-promoting rhizobacteria (PGPR) and may contribute to enhancing plant growth and serve as biocontrol agents against P. ananatis.