Salim Widono
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret

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Antagonisme Bacillus terhadap Infeksi Layu Fusarium pada Bibit Pisang Hasil Kultur Jaringan Hadiwiyono Hadiwiyono; Arief Widyantoro; Salim Widono
Agrosains : Jurnal Penelitian Agronomi Vol 15, No 1 (2013): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.832 KB) | DOI: 10.20961/agsjpa.v15i1.18990

Abstract

Fusarium wilt (Fusarium oxysporum f.sp. cubense) is an important disease in banana. Fusarium wilt was hard to control because the pathogen can survive in many kind of soils type although there is no host. Therefore, overcoming the disease is urgently needed such as biological control. The endophytic Bacillus of banana was begun to use as antagonist agent to the pathogen. This research aimed to study the mechanism of antagonism and physiological character of Bacillus. There were 27 Bacillus isolates examined in-vitro to test the production of IAA, HCN, chitinase, pectinase, and antagonism. The top ten isolates based on the test in vitro were used to test in planta. The research showed that Bacillus were able to produce IAA, HCN, chitinase, pectinase, and able to retard the growth of Foc colony. The application of isolat B25 on banana seedling could decrease the disease intensity but still unable to prevent the fusarium wilt infection.
Efektivitas Minyak Atsiri Serai Untuk Pengendalian Penyakit Mosaik Kacang Panjang Supyani Supyani; Salim Widono; Ulfaizah Ulfaizah
Agrosains : Jurnal Penelitian Agronomi Vol 19, No 2 (2017): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.793 KB) | DOI: 10.20961/agsjpa.v19i2.20912

Abstract

Production of cowpea has declined, one of them caused by virus infection that caused mosaic disease which was still difficult to controlled. This study aimed to determine the effectivity of antiviral compounds on lemongrass essential oils to development of mosaic disease and the relationship to growth and development of cowpea that infected. This study used Completely Randomized Design (CRD) with two factors, they are the time of application and concentration of lemongrass essential oil. Data were analyzed using F test and DMRT at level of 5%. Results showed that application of lemongrass essential oil at concentration of 1,8% before planting (soaking seeds) tend to delaying the emergence of mosaic disease symptoms. Application of lemongrass essential oil at all of concentration that used, can inhibiting the disease intensity up to 31.39%. However, apllication of lemongrass essential oils in plants infected with a virus that causes mosaic has not able to improve plant growth and development.