Sri Hartati
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Sebelas Maret

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Journal : Journal of Biodiversity and Biotechnology

GROWTH ORCHID (♀) DENDROBIUM LINIALE X (♂) DENDROBIUM BIGGIBUM ON MEDIUM NAA AND ORGANIC MATERIAL IN VITRO Sri Hartati; Heni Purwanti; Nandariyah Nandariyah
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.60092

Abstract

The research aimed to study the influence of the application of NAA concentration, the addition of organic materials and a combination of both on media Knudson C towards the growth of orchid plantlet (♀) Dendrobium liniale >< (♂) Dendrobium biggibum. The purpose of using media Knudson C in culture in vitro is to optimize the growth of orchid plantlet Dendrobium liniale and Dendrobium biggibum. The experiments were carried out in the Laboratory of Plant Physiology and Biotechnology Faculty of Agriculture, Sebelas Maret University Surakarta. The research used Complete Randomized Design (CRD) with 2 treatment factors, factor I was the concentration of NAA (0 ppm, 1 ppm, 3 ppm and 5 ppm); and factor II was the organic materials (coconut water, banana, potatoes and sweet potatoes). In total there were 20 treatment combinations . Each combination was repeated four times. The results showed that the combination of sweet potato and NAA 5 ppm produced the following characteristics: the fastest root growth, most root length, and the largest number of leaves; while the combination of banana extracts and NAA 5 ppm produced a number of roots, the shoots appear most rapidly, and the highest number of shootsKeywords: genetic diversity; tissue culture; medium;
Application Cow Manure and Mychorriza to Physiological of Biduri (Calotropis gigantea) Sri Hartati; Samanhudi Samanhudi; Muji Rahayu; Mochammad Danny Sukardan; Nadia Alifia Rahma
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.61273

Abstract

Biduri (Calotropis gigantea) was a wild plant that had many benefits in the health and textile industries. In the health sector, biduri was used as a herbal plant, while in the textile industry, used as the main raw material. Biduri could be used as a substitute for kapok as the main raw material, therefore it was necessary to develop cultivation technology to met the needs. This research was conducted at the Jumantono Field Laboratory of Faculty of Agriculture UNS, Karanganyar using a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely cow dung fertilizer, and mycorrhizae. Cow dung fertilizer consists of 0 ton/ha, 10 ton/ha, and 20 ton/ha. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 36 treatment combination that consist of 12 treatment combination repeated 3 times. The interaction between cow dung did not significantly affect all physiological observation variables. The results showed that the application of 20 ton/ha of cow dung fertilizer gave the best results on the width of stomata openings, the rate of photosynthesis, and the rate of transpiration. Inoculation with mycorrhizal fungi had no significant effect on all observations of physiological variables Keywords: Calotropis gigantea, cow manure, mycorrhiza, physiological
The Effect of Dosage and Frequency of Leri Water Spraying on Phalaenopsis Fuller's Pink Stripe Orchid Growth of Aclimatisation Sri Hartati; Ongko Cahyono
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61198

Abstract

The phalaenopsis hybrid has become an economically important flower in the horticultural sector. Phalaenopsis Fuller's Pink Stripe Orchid is a hybrid orchid resulting from a cross between Phalaenopsis Fuller's Pink Swallow and Phalaenopsis Fullers Black Stripe. Rice washing water (leri) is a residue that accumulates when washing rice. This residue is usually thrown away when it is actually rich in various organic and mineral compounds. Waste that is wasted in vain can be used as nutrients for the growth of orchid plants. This research was conducted to study the effect of dose and frequency of leri water spraying on the growth of Phalaenopsis Fuller's Pink Stripe orchid at the vegetative stage. This study consisted of 10 treatments, namely giving 5 ml of water every 2 days (L1), 10 ml every 2 days (L2), 15 ml every 2 days (L3), 5 ml every 4 days (L4). a dose of 10 ml every 4 days (L5), a dose of 15 ml every 4 days (L6), a dose of 5 ml every 6 days (L7), a dose of 10 ml every 6 days (L8), a dose of 15 ml every 6 days (L9), and control treatment (L0). Each treatment was repeated 3 times. Based on the results of the study, it are often all over that there's no important interaction between the dose and also the frequency of administration of Leri water on the growth and performance of the Phalaenopsis Fuller's Pink Stripe.Keywords: Rice Wash Water, Phalaenopsis Fuller's Pink Stripe, vegetative growth