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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
A. Setiyono
FKH IPB
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Effect of fermented Jatropha curcas meal combined with enzymes on metabolizable energy, retention of N, P, Ca and digesteble crude fiber Yusriani, Yenni; Toharmat, T.; ., Sumiati; Wina, E.; Setiyono, A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.713 KB) | DOI: 10.14334/jitv.v16i3.610

Abstract

Jatropha curcas meal (JCM) contain high protein, but its utilization as feed ingredient is limited by the presence of several anti nutritive and toxic compounds. A research has been conducted in the Faculty of Animal Husbandry IPB to determine the values of metabolizable energy, crude fiber digestibility, retention of nitrogen, calcium and phosphorous of JCM fermented using Rhizopus oligoporus or of fermented JCM suplemented with enzymes on chickens. In this experiment 25 of ten weeks old chickens were used (20 chickens were fed experimental diets, and 5 chickens were used to measure endogenous energy). A Completely Randomized Design (CRD) with 5 treatments and 4 replications was used in this experiment. The experimental diets were R0 = basal diet without JCM; R1 = the diet contained unprocess JCM 5%; R2 = the diet contained fermented JCM 5%+ cellulase 20.000 U/kg; R3 = the diet contained 5% of fermented JCM + phytase 1000 FTU/kg; R4 = the diet contained 5% of fermented JCM+ cellulase 20.000 U/kg + phytase 1000 FTU/kg. The parameters measured were Metabolizable Energy (ME, Men, TME, TMEn) digestibility of crude fiber and retention of calcium, phosphorus and nitrogen. The result indicated that fermented JCM suplemented enzym phytase, cellulase as well as enzym combination increased metabolizable energy. Nitrogen retention was improved highest significantly (P < 0.01) by feed of fermented JCM suplemented enzyme compared to control diet or diet containing untreated JCM. The fermented JCM suplemented with phytase enzyme (R3) gave the highest nitrogen retention (76.73%.). The highest value of digested crude fibre (24.65%) was on the diet containing fermented JCM suplemented with cellulase (R2). The calcium retention improved significantly (P < 0.05) with diet containing fermented JCM supplemented enzyme compared to that of the diet containing untreated JCM. It can be concluded that fermented JCM supplemented enzymes cellulase, phytase and its combination increased the value of metabolizable energy, digestibility of crude fiber and nitrogen retention. Key Words: Chicken, JCM Fermented, Enzymes, Metabolic Energy
Curcumin effect on Bleomycin-induced pulmonary fibrosis in Mus musculus Rahmi, Annisa; Setiyono, A.; Juniantito, V.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.991 KB) | DOI: 10.14334/jitv.v20i2.1169

Abstract

Curcumin, a curcuminoid compound of turmeric has been demonstrated to have anti-oxidant and anti-inflammatory properties. Bleomycin (BLM) is an anti-cancer drug induced pulmonary fibrosis in human and animals. This study was aimed to investigate biological effects of curcumin on bleomycin-induced pulmonary fibrosis in mice (Mus musculus) through pathomorphological assessment. In this study, 16 mice ddy strain were divided into four groups, namely (i) control, mice were subcutaneously (SC) injected with 100 µl sterilized aquadest in dorsal skin, (ii) BLM group, injected SC with 100 µl of 1 mg/ml BLM in dorsal skin, (iii) Curcumin (CMN) group, mice were intraperitoneally (IP) injected with 100 mg/kg body weight (BW) curcumin dissolved in 0,5% carboxy methyl cellulose (CMC) and injected with 100 µl sterilized aquadest SC, (iv) BLM+CMN group, injected SC with 100 µl of BLM 1 mg/ml and injected IP with 100 mg/kg BW CMN in 0,5% CMC. All treatments were performed daily for four weeks period. The lung samples were collected and fixed in buffered neutral formalin (BNF) 10%. Histopathological evaluation was performed with hematoxylin-eosin (HE) and Masson’s trichrome (MT) stains. The results showed that BLM treatment significantly increased fibrosis area and alveolar wall area fraction as compared to control. OIn the other hand, CMN treatment significantly reduced fibrosis area and alveolar wall area fraction in mice treated with BLM. In conclusion, our study showed that CMN treatment may inhibit lung fibrogenesis in BLM-induced pulmonary fibrosis. Key Words: Bleomycin, Curcumin, Mice, Pulmonary Fibrosis
The potency of sericin as an alternative protein in collection and maturation media to support in vitro bovine oocyte maturation Karja, N. W. K.; Satrio, F. A.; Setiyono, A.; Prasetyaningtyas, W. E.; Fahrudin, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.224-230

Abstract

The purpose of this study was to evaluate the potency of sericin as a substitute for BSA (bovine serum albumin) to support the in vitro maturation of bovine oocytes. Cumulus-oocytes complexes were collected with BSA or 0,1% sericin, and then matured either in media with BSA or sericin. The maturation rate was evaluated based on the meiotic status of oocytes. The incidence of DNA fragmentation in oocytes was assessed by TUNEL. The percentage of oocytes reaching the MII stage in the media either with BSA or sericin was significantly higher than those of oocytes collected and matured in BSA only. Although the maturation rate of oocytes collected with BSA and then matured with sericin was comparable to oocytes in BSA-BSA groups (P>0.05) the rate was similar to oocytes collected with sericin and then even matured with BSA or sericin (P>0.05). There was no significant difference in the incidence of DNA fragmentation among the treatment groups (P>0.05), the indices of DNAfragmented oocytes were found around 17-20 %. In conclusion, Sericin has the potency to replace BSA as a source of protein either in collection media and/or in maturation media as well as potentially preventing the incidence of DNA fragmentation in oocytes.
Co-Authors Annisa Rahmi E. Wina M. Fahrudin N. W. K. Karja, N. W. K. Prasetyaningtyas, W. E. Satrio, F. A. Sumiati . T. Toharmat V. Juniantito, V. Yenni Yusriani