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PROSPEK ENZIM DAN LIMBAH LIGNOSELULOSA UNTUK PRODUKSI BIOETANOL Trisanti Anindyawati
JURNAL SELULOSA Vol 44, No 01 (2009): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4599.154 KB) | DOI: 10.25269/jsel.v44i01.149

Abstract

Substitution bioethanol as one of energy source has been selected as an alternative source for the fossil fuel substitution. The arricultural and industrial waste can be used for the production of bioethanol. The main component in those waste materials is lignocelluloses that contained cellulose, hemicelluloses and lignin. Lignocellulose will be the main source for bioethanol production in the long term. Several enzymes have been recorded in degrading lignollulose wich are cellulolytic, hemicellulolytic as well as lignolytic. The main enzyme which has the most important role in bioethanol production are complex enzyme which degrade lignocelluloses. Bioethanol prodaction is much affected by raw materials composition, type of microorgranisms, and the fermentation condition used.Key words: lignocelluloses, cellulase, hemicellulase, lignin degrading enzyme, bioethanolINTISARI Bioetanol merupakan salah satu energy alternative pengganti minyak bumi. Bahan limbah pertanian dan industry dapat digunakan untuk produksi bioetanol. Komponen utama pada limbah pertanian dan industri yang digunakan untuk produksi bioetanol adalah lignoselulosa yang terdiri dari selulosa, hemiselulosa dan lignin. Lignoselulosa merupkan bahan utama produksi bioetanol untuk jangka panjang. Enzim yang berperan dalam degradasi lignoselulosa adalah enzim yang bersifat selulotik, hemiselulolitik dan lignolitik. Enzim utama yang berperan penting pada produksi bioetanol merupakan enzim kompleks yang mampu mendegradasi lignoselulosa. Produksi bioetanol sangat di pengaruhi oleh komposisi bahan baku, jenis mikroorganisma dan kondisi fermentasi yang digunakan.Kata Kunci : lignoselulosa, selulase, hemiselulase, enzim pendegradasi lignin, bioetanol
Characterization of Protease Crude Extract from Indigenous Lactic Acid Bacteria and the Protein Degradation Capacity in Local Tuber and Cereal Paste Flour Tatik Khusniati; Nanda Sabbaha Nur Kasfillah; Vilya Syafriana; Resti Sofia Zahara; Padmono Citroreksoko; Sulistiani Sulistiani; Trisanti Anindyawati
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.694 KB) | DOI: 10.14203/jkti.v21i1.419

Abstract

Protease hidrolyzed protein in flour in order to more digest by human ulcer. Lactobacillus plantarum B110 and Lactobacillus satsumensis are indigenous lactic acid bacteria that produce protease. The objective of this research is to characterization of protease crude extract from indigenous lactic acid bacteria and the protein degradation capacity in local tuber and cereal paste flour. Tuber and cereal flour used were purple sweet potato (Dioscorea alata), cassava (Manihot esculenta), rice (Oryza sativa), corn (Zea mays) and wheat (Triticum) as comparison. Proteaseactivity was tested by Horikoshi method (1971) and protein degradation was by formol titration. Research results showed that optimum activities and stabilities of Lactobacillus plantarum B110 were at pH: 7.5, 45oC and pH:5.0-8.0, 35-50oC, while that L. satsumensis EN 38-32 were at pH: 7.0, 40oC and pH:6.0-8.0, 20-45oC. Increases in protein degradation capacity of the paste flour additional proteases crude extract from L. plantarum B110 were 0.0838% (purple sweat potato), 1.3299% (cassava), 0.5834% (corn), 0.7499% (rice) and 1.5551% (wheat as comparison); while that L. satsumensis EN 38-32 were 0.20% (purple sweet potato), 0.32% (cassava), 0.87% (corn), 1.17% (rice). Based on increases in protein degradation capacity, protease crude extract from L. plantarum B110  and L. satsumensis EN 38- 32 were sequently better to hidrolyze protein of cassava and rice paste flour than thatother tuber and cereal.
The Evaluation of Substrates and Trichoderma sp. Isolates for Cellulase Production Eka Triwahyuni; Yosi Aristiawan; Novita Ariani; Haznan Abimanyu; Trisanti Anindyawati
Jurnal Kimia Terapan Indonesia Vol 20, No 1 (2018)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.106 KB) | DOI: 10.14203/jkti.v20i1.384

Abstract

AbstractAs higher interest was on the lignocellulose-based or second generation bioethanol production, the research was then more focused on the production of cellulase, especially on the domestic enzyme. Trichoderma sp. is considered as one of the most efficient producer of cellulase. This study was conducted to investigate the performance of Trichoderma sp. on a variety of substrates to produce cellulase. Three types of substrate variations and three types of Trichoderma sp. were used in this experiment. The substrate used were wheat bran, rice bran and oil palm empty fruit bunches (EFBs), whereas Trichoderma sp. isolates were encoded as T004, T051 and T063. Production of cellulase was made by solid fermentation for 7 days. The analysis of cellulase activity was done by National Renewable Energy Laboratory (NREL) method for filter paper assay. The results showed that the type of substrate affected the performance of Trichoderma sp. All types of fungus produced cellulase on wheat bran substrate with activity of 0.52 FPU /ml for T004, 0.23 FPU/ml for T051 and 0.27 FPU /ml for T063. With the rice bran substrate and EFBs, only T004 could produce cellulase and the enzyme activity analyzed were 0.08 FPU /ml and 0.008 FPU/ml respectively. Optimation of the buffer addition on enzyme extraction process produces the highest activity 0.85 FPU/mL for T004 with wheat bran substrate. Keywords: cellulase, EFBs, rice bran , Trichoderma sp. , wheat bran