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All Journal Aquacoastmarine ALCHEMY Jurnal Penelitian Kimia Journal of Saintech Transfer Jurnal Agrisep Biodiversitas Journal of Biological Diversity
Wahyu Haryati Maser
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology Other

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APPLICATION OF HPLC-BASED METABOLOMICS TO IDENTIFY RETENTION TIMES ANTIBACTERIAL COMPONENTS OF Stapylococcus aureus FROM KECOMBRANG FLOWER EXTRACT (Etlingera elatior) Wahyu Haryati Maser; Nancy Dewi Yuliana
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 2 (2017): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.2.4890.239-249

Abstract

Kecombrang (Etlingera elatior) is a well-known herb in Indonesia that is widely used in traditional medicine and as a flavour in local dishes. The aim of this study was to identify retention time of antibacterial components from kecombrang flower extract (Etlingera elatior) by HPLC-based metabolomics method. The antibacterial activity of ethanol extracts, chloroform, and water fractions was assessed by well-diffusion agar method against Staphylococcus aureus. Only chloroform fraction showed medium inhibition activity against Staphylococcus aureus. The HPLC chemical profiles of the fractions were then correlated to their antibacterial activity profile by means of Orthogonal Projection to Latent Sructure (OPLS) analysis. Peak area with retention time of 0.96 - 1.12 min present in chloroform fraction at UV 250 nm was found to significantly correlate to the antibacterial activity (Y related coefficient value 0.73).
A review of the enzymes utilization in the feed for indigenous Indonesian fish : enzymes utilization in the feed for indigenous Indonesian fish Maser, Wahyu Haryati; Muhtadi, Ahmad; Ritonga, Ajuan; Almira, Della; Panjaitan, Febriyanti; Sari, Nurmala; Sinurat, Ries Ruth Rytond; Kembaren, Natasyha Pebrina Br Sembiring; Purba, Theresia Widi Astuti; Nababan, David; Andari, Ella; Harahap, Muhamad Haikal Firyaldi; Pasaribu, Rihan Addilah
AQUACOASTMARINE: Journal of Aquatic and Fisheries Sciences Vol. 4 No. 1 (2025): AQUACOSTMARINE: Journal of Aquatic and Fisheries Sciences
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jafs.v4i1.20246

Abstract

Enzymes play a vital role in improving nutrient absorption and digestion efficiency in fish feed, particularly for indigenous Indonesian fish species. This review aimed to comprehensively evaluate the current state of enzyme utilization in feed for native Indonesian fish, highlighting its benefits, challenges, and potential contributions to the advancement of sustainable aquaculture. It examined the role of endogenous enzymes and various exogenous enzymes, such as protease, lipase, phytase, Non-Starch Polysaccharides (NSP) enzymes, and multi-carbohydrase complexes, in enhancing feed efficiency and fish growth. The integration of these enzymes into feed formulations offers significant advantages in boosting growth performance, optimizing feed conversion efficiency, and promoting environmental sustainability. Additionally, this review discussed the potential benefits and challenges associated with enzyme application in fish feed. Looking ahead, the development of enzyme utilization in aquafeed holds great promise for enhancing the sustainability, efficiency, and productivity of the aquaculture sector. This review provides valuable insights into the potential of enzyme supplementation to maximize feed utilization for native Indonesian fish species, outlining both its advantages and challenges, while also exploring its future prospects in driving cost-effective and eco-friendly aquaculture practices.
Strategi Perencanaan Dan Pengembangan Industri Pariwisata di Kecamatan Panai Hilir, Kabupaten Labuhanbatu Ajuan Ritonga; Wahyu Haryati Maser; Dian Habibie; Sri Wahyuni
Jurnal Agrisep Vol. 26 No. 2 (2026): Volume 26 Nomor 2 Desember 2025
Publisher : Departemen Agribisnis, Fakultas Pertanian, Universitas Syiah Kuala associated with AAI (Asosiasi Agribisnis Indonesia). Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam, Banda Aceh, 23111, Indonesia. Email: agrisep@usk.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agrisep.v26i2.345

Abstract

This study aimed to formulate planning and development strategies for the tourism industry in Panai Hilir District, Labuhanbatu Regency, North Sumatra, using SWOT and QSPM methods. The region holds significant natural and cultural tourism potential, but it has not been optimally developed due to limitations in infrastructure, promotion, and community involvement. This research employed a descriptive approach through the collection of primary and secondary data, which were then analyzed using IFAS, EFAS, SWOT, and QSPM matrices. The SWOT analysis resulted indicate strengths such as natural and cultural richness and community support, and weaknesses including limited facilities and infrastructure. Opportunities included government policies and community support, while threats involve competition from other regions and economic-political conditions. The QSPM analysis produced four main strategies, with the highest priority being the improvement of living standards and community welfare through the tourism sector, followed by infrastructure development, management system enhancement, and capacity building of human resources. These findings are expected to serve as a strategic policy foundation for sustainable tourism development in Panai Hilir and contribute to local economic growth and cultural preservation.
Community Service Activities on Developing Instant holat Spice Products for Housewife Groups in Medan Johor and Medan Tuntungan, Medan City Wahyu Haryati Maser; Emma Marsella; Ajuan Ritonga; Mangaraja Halongonan Harahap
Journal of Saintech Transfer Vol. 9 No. 1 (2026): Journal of Saintech Transfer
Publisher : Talenta Publisher Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jst.v9i1.20329

Abstract

This community service aimed to assist housewife groups in Medan Johor and Medan Tuntungan, Medan City, in developing instant holat spice products. Holat, a traditional Mandailing dish, holds cultural significance but is time-intensive and difficult to prepare, as well as the raw materials are difficult to find in urban areas. The initiative sought to preserve local culinary traditions while providing an economic opportunity for the target group. Activities included training sessions on ingredient selection, production techniques, food safety, packaging, and marketing strategies. Through these efforts, participants gained skills to produce instant holat spices and market them effectively. The program successfully empowered the participants by fostering entrepreneurship and promoting traditional cuisine spices in a modern way, contributing to both cultural preservation and economic improvement.
The pattern of Etlingera elatior cultivation in agroforestry systems and its use as traditional medicines and food by local people of Kabanjahe, North Sumatra, Indonesia AGUS PURWOKO; HELTIMALA TURNIP; WAHYU HARYATI MASER
Biodiversitas Journal of Biological Diversity Vol. 20 No. 7 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200728

Abstract

Abstract. Purwoko A, Turnip H, Maser WH. 2019. The pattern of Etlingera elatior cultivation in agroforestry systems and its use as traditional medicines and food by local people of Kabanjahe, North Sumatra, Indonesia. Biodiversitas 20: 1998-2003. Etlingera elatior or kecombrang (Indonesian name) is a native plant of Indonesia. In Kabanjahe, this plant is planted in agroforestry systems. The purposes of this study were to describe the pattern of Etlingera elatior cultivation in agroforestry systems, and to document the use of Etlingera elatior as medicines and food by the local people of Kabanjahe, North Sumatra. Data were collected with field observations and interviews, and the collected data were analyzed qualitatively. The results showed that kecombrang was planted in agroforestry systems in three patterns, namely (i) kecombrang, cinnamon, and coffee, (ii) kecombrang, coffee, and bamboo, and (iii) and kecombrang, avocado, and vegetables. Kecombrang has been used as medicines for cough, wound, eye problems, fever, low breast milk production, odor, and hair thinning. In addition, kecombrang has also been used as a food ingredient in ubi tumbuk, ikan arsik, sambal kincung, getah tasak telu, cipera, terong dotak, gat-gat, and gulen manuk. With this research, it is suggested that agroforestry systems be developed to improve economic benefits for the local people of Kabanjahe.
Evaluation of proximate composition, microbial load and sensory characteristics of instant holat as traditional spice in North Sumatra, Indonesia Wahyu Haryati Maser; MUHAMMAD ZULHAM EFENDI SINAGA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 8 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220841

Abstract

Abstract. Maser WH, Sinaga MZE. 2021. Evaluation of proximate composition, microbial load and sensory characteristics of instant holat as traditional spice in North Sumatra. Biodiversitas 22: 3440-3445. Holat is the name of traditional food from North Sumatra, Indonesia which has astringent flavor derived from the bark of Phyllanthus emblica. The aims of this study were to determine the proximate composition and evaluate microbial load, and sensory characteristics of instant holat spice. There were 12 treatments, namely the combination of different amounts of bark (15 g, 25 g, 35 g, 45 g) and drying time (20 h, 22 h, 24 h). The proximate composition (moisture, ash, protein, fat, crude fiber, fat, and total carbohydrate content), microbial analysis (total plate count; yeast and mold count), and sensory characteristics (aroma, color, taste, and overall acceptability) were carried out. The results showed that water, protein, fat, crude fiber, and total carbohydrates contents were all significantly different (p < 0.05), except ash content. There was no yeast, mold, and bacterial growth on total plate count. There were no significant differences in all sensory characteristics (aroma, color, taste, and overall acceptability) of instant holat before and after brewing (p ? 0.05). The treatment of F4-24 was the best formula and has the highest score that might be developed as a product of instant holat spice. Further study should be conducted to determine the effect by increasing the amount of bark and evaluating its bioactivity.
Effect of different extraction solvents on the yield and enzyme inhibition (a-amylase, a-glucosidase, and lipase) activity of some vegetables Wahyu Haryati Maser; Nur Maiyah; Muralidharan Nagarajan; Passakorn Kingwascharapong; Theeraphol Senphan; Ali Muhammed Moula Ali; Sri Charan Bindu Bavisetty
Biodiversitas Journal of Biological Diversity Vol. 24 No. 6 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240625

Abstract

Abstract. Maser WH, Maiyah N, Nagarajan M, Kingwascharapong P, Senphan T, Ali AMM, Bavisetty SCB. 2023. Effect of different extraction solvents on the yield and enzyme inhibition (a-amylase, a-glucosidase, and lipase) activity of some vegetables. Biodiversitas 24: 3320-3331. The present study investigated the in vitro inhibition of a-amylase, a-glucosidase, and lipase, and antioxidant activities (DPPH, metal chelating, and FRAP) of Allium cepa L., Apium graveolens L., Coriandrum sativum L., and Petroselinum crispum (Mill.) Fuss using solvent 80% ethanol, absolute ethanol, acetone, methanol, n-hexane, chloroform, and hot water. The total phenolic content (TPC) and the FTIR and GC-MS characterization spectra were compared with the activities. The TPC of 80% ethanol extract in A. graveolens L. was the highest among the extracts, with 23.78 mg GAE/g extract. The 80% ethanol extract exhibited the most potent antioxidant activity on DPPH radical scavenging (A. graveolens, 125.57 mg AEAC/g extract), metal chelating (C. sativum, 92.85 mg EECC/g extract), and FRAP activities (C. sativum, 46.98 mg AEAC/g extract). The 80% ethanol extract showed the highest anti-a-amylase (P. crispum, 30.61 mmol ACE/g extract) and anti-a-glucosidase (A. cepa, 595.28 mmol ACE/g extract) activities. In comparison, the absolute ethanol extract of C. sativum showed the highest anti-lipase activity (42.10% inhibition). According to the FTIR spectra, the 80% ethanol extract of the four green leafy vegetables is predicted to have several active compounds. GC-MS identified the compounds responsible for the activity. Studies on the recovery potential of active compounds as oral agents to treat diabetes mellitus with certain solvents are very useful in producing oral agents and functional foods to prevent diabetes mellitus.
Effect of different drying methods on phenolic content, antioxidant, antidiabetic, anti-obesity, and inhibition kinetic properties of selective green leafy vegetables WAHYU HARYATI MASER; SUPATRA KARNJANAPRATUM; PASSAKORN KINGWASCHARAPONG; KARTHIKEYAN VENKATACHALAM; ALI MUHAMMED MOULA ALI; SRI CHARAN BINDU BAVISETTY
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240932

Abstract

Abstract. Maser WH, Karnjanapratum S, Kingwascharapong P, Venkatachalam K, Ali AMM, Bavisetty SCB. 2023. Effect of different drying methods on phenolic content, antioxidant, antidiabetic, anti-obesity, and inhibition kinetic properties of selective green leafy vegetables. Biodiversitas 24: 4896-4909. The present study assessed the impact of dehydration drying, oven drying, and freeze drying on Total Phenolic Content (TPC), antioxidant activities (DPPH, metal chelating, and FRAP), and the inhibition activity of a-amylase, a-glucosidase, and lipase in selected green leafy vegetables, namely celery (Apium graveolens L.), coriander (Coriandrum sativum L.), parsley (Petroselinum crispum (Mill.) Fuss), and spring onion (Allium cepa L.). FTIR analysis was conducted to examine the functional groups, GC-MS was utilized to characterize the compounds, and a kinetic study of a-glucosidase inhibition was undertaken to assess its inhibitory mode as an antidiabetic agent. Freeze drying exhibited the highest extraction yield (17.89% to 26.15%). However, freeze-drying showed lower drying efficiency, TPC, and bioactivities. Oven drying showed the highest bioactivities, including antioxidant and enzyme inhibitory activities. Spring onion demonstrated the highest bioactivity with dehydration drying, particularly in a-glucosidase inhibitory activity. The results notified that oven drying can be highly suitable for green leafy vegetables with thinner structures, facilitating an efficient drying process. On the other hand, dehydration drying is better suited for thicker green leafy vegetables with the appropriate heat pump intensity. The results for bioactivities were aligned with the observed inhibition mechanism, functional groups, and bioactive compounds in the samples analyzed via FTIR and GC-MS analysis. Therefore, selecting the drying method for a specific sample should consider its unique characteristics to ensure optimal bioactivity.
Co-Authors AGUS PURWOKO Ahmad Muhtadi Ajuan Ritonga ALI MUHAMMED MOULA ALI Ali Muhammed Moula Ali Almira, Della Andari, Ella Emma Marsella Febriyanti Panjaitan Habibie, Dian Harahap, Muhamad Haikal Firyaldi HELTIMALA TURNIP KARTHIKEYAN VENKATACHALAM Kembaren, Natasyha Pebrina Br Sembiring Mangaraja Halongonan Harahap MUHAMMAD ZULHAM EFENDI SINAGA Muralidharan Nagarajan Nababan, David Nancy Dewi Yuliana Nur Maiyah Nurmala Sari Pasaribu, Rihan Addilah PASSAKORN KINGWASCHARAPONG Passakorn Kingwascharapong Purba, Theresia Widi Astuti Sinurat, Ries Ruth Rytond SRI CHARAN BINDU BAVISETTY Sri Charan Bindu Bavisetty Sri Wahyuni SUPATRA KARNJANAPRATUM Theeraphol Senphan