Aaron T. Sihombing
Department of Urology, Faculty of Medicine/Padjadjaran University, Hasan Sadikin Hospital, Bandung, Indonesia.

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ESTROGEN-TESTOSTERONE RATIO IN PLEXUS PAMPINIFORM OF NORMAL RABBITS AND RABBITS WITH LEFT ARTIFICIAL VARICOCELE Simorangkir, Lambok; Sihombing, Aaron T.; Noegroho, Bambang S.
Indonesian Journal of Urology Vol 20 No 1 (2013)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v20i1.40

Abstract

Objective: To evaluate estrogen-testosterone ratio in rabbits with a left artificial varicocele. Material& Method: Eight rabbits were divided into two groups.The first group underwent ligation of left renal vein to induce artificial varicocele and the second group underwent a sham operation as control group. Eight week after ligation, blood samples were taken from the pampiniform plexus in both groups. Serologic examination (ELISA) was performed to measure testosterone and estrogen level. The t-test was used to compare the differences of the estrogen-testosterone ratios, a p value < 0,05 is considered significant.Results: Testosterone level was increased with 279% and estrogen level was increased with 33% blood from the pampiniform plexus in group 1 compared with group 2 (normal rabbits). The ratio of estrogen-testosterone between the control group and the artificial varicocele group were 1 : 3. Differences of the ratio between two groups were statistically significant (p < 0,05). Conclusion: Estrogen-testosterone ratio was lower in varicocele rabbit compared with normal rabbits.Keywords: Estrogen-testosteron ratio, left artificial varicocele.
EFFECT OF TESTOSTERONE REPLACEMENT ON EPITHELIAL AND STROMAL TISSUE IN PROSTATIC LOBE IN ORCHIDECTOMIZED WISTAR Irawan, Yudi; Sihombing, Aaron T.
Indonesian Journal of Urology Vol 20 No 1 (2013)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v20i1.41

Abstract

Objective: To evaluate the effect of testosterone replacement on epithelial and stromal changes of prostatic lobes in castrated wistar rats. Material & Method: The subjects were 30 wistars equally assigned to castrated + testosterone replacement group (n = 10), castrated group (n = 10), and control group (n = 10). After 60 days, prostatectomy was performed in all rats and prostatic specimens were analyzed by haematoxylin eosin (HE) staining under microscope. Semi–quantitative analysis was performed by evaluating growth of epithelial structure and loss of fibromuscular stroma. Results were analyzed using ANOVA test method for normally distributed data. The statistical analysis was performed using SPSS. Results: There was significant reversibility in castration + testosterone replacement groups in all prostatic lobes compared with castration groups (p = 0,010).There were 5 rats showing normal structure of prostate gland compared to control groups in all prostatic lobes (50%), and 5 showed hyperplasia in all prostatic lobes (50%). Conclusion: Testosterone deprivation can cause prostatic atrophy. Dominant atrophy was found in ventral and lateral lobes. Testosterone replacement can prevent atrophy in all prostatic lobes regardless of specific prostatic lobes.Keywords: Testosterone deprivation, testosterone replacement, prostatic lobes.
ATHEROSCLEROTIC PLAQUE OF RENAL VASA RECTA ON PATIENT WITH CALCIUM STONE Pramod, Sawkar Vijay; Sugandi, Suwandi; Sihombing, Aaron T.; Makmuri, Makmuri
Indonesian Journal of Urology Vol 20 No 1 (2013)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v20i1.45

Abstract

Objective: We would like to identify whether atherosclerotic plaque inside renal vasa recta correlates with the formation of calcium stone. Material & Method: Samples are taken from kidney tissue removed by nephrectomy due to stone and non-stone disease. Histopathological examination with specific staining was performed by a single pathologist to find the atherosclerotic plaques inside the renal vasa recta. The result of each group was compared, and then analyzed using the Chi square test/Fischer exact test (SPSS ver.16.0; p < 0,05). Results: We found that the risk of calcium lithiasis is two-fold higher in the group with atherosclerotic plaques inside the renal vasa recta. (PR = 2,15; p < 0,05). Conclusion: Calcium stone are more likely to occur on patients with renal vasa recta atherosclerotic plaque, as possible sequelae of vascular injury.Keywords: Atherosclerotic plaque, vasa recta, calcium renal stone.