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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science
Kamaruzaman Sijam
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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PRODUCTION OF POLYCLONAL ANTIBODY TO THE COAT PROTEIN OF CITRUS TRISTEZA VIRUS IN CHICKEN EGGS ., Nurhadi; Sijam, Kamaruzaman; Sulaiman, Inon
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Citrus tristeza virus (CTV) is one of the most destructive diseases in many citrus growing areas of Indonesia. Effective strategies for controlling CTV depend on diagnostic procedure namely enzyme-linked immunosorbent assay (ELISA). Study aimed to purify the CTV antigen and produced its polyclonal antibody. Virion of the severe CTV isolate designated UPM/T-002 was concentrated by polyethylene glycol (PEG) precipitation combined with low speed centrifugation. Semipurified antigen was further purified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). The specific coat protein (CP) band of CTV with molecular weight of 25 kD was excised and eluted using elution buffer containing 0.25 M Tris-HCl pH 6.8 + 0.1% SDS, then used as antigen for injectioninto 6-month-old female of White Leghorn chicken. Results, showed than the specific polyclonal antibody raised against the 25-kDa CP had a titer of approximately 104, gave low background reaction with healthy plant sap and reacted specifically with CTV isolates. The reaction was equally strong for a severe, a moderate, a mild, and a symptomless isolate, suggesting a broad reaction range of this antibody toward different CTV isolates. Optimal virus titer can be obtained since virus loss during purification could be minimized and the highly purified antigen as an immunogen could be obtained by cutting out the CP band from SDS-PAGE gels. Large amount of highly titer of CTV antibody can be produced in chicken egg. The simplicityof the procedure makes it economically acceptable and technically adoptable because the antibody can be produced in basic laboratory.
APPLICATION METHOD OF ANTIMICROBIAL SUBSTANCES FOR THE CONTROL OF SCHIZOPHYLLUM COMMUNE FR. CAUSING BROWN GERM AND SEED ROT OF OIL PALM Dikin, Antario; Sijam, Kamaruzaman; Seman, Idris Abu
BIOTROPIA Vol. 15 No. 1 (2008): BIOTROPIA Vol. 15 No. 1 June 2008
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.024 KB) | DOI: 10.11598/btb.2008.15.1.4

Abstract

Biological seed treatment promotes to save the environment from toxic chemicals in the agricultural practices. Schizophyllum commune is one of the important seedborne pathogenic fungi causing brown germ and seed rot of oil palm which required effective and efficient treat­ment based on environmental friendly approaches. Anti-microbial substances are extracted from antagonistic bacteria of B. multivorans and M. testaceum after mass production in the liquid media. Application method of anti-microbial substances for the control of Schizophylllum commune was done by seed dipping for 30 minutes and vacuum infiltration at 400 mm Hg. vac. for 2 min. in supernatant of anti-microbial substances diluted in sterilized distilled water with concentra­tion ratio of 1:4. Application method using anti-microbial substances from antagonistic bacteria inhibited the growth of pathogenic fungus, enhanced seed germination, and without causing any abnormal growth of oil palm seedlings.
ANTAGONISTIC BACTERIA AGAINST SCHIZOPHYLLUM COMMUNE FR. IN PENINSULAR MALAYSIA DIKIN, ANTARJO; SIJAM, KAMARUZAMAN; KADIR, JUGAH; SEMAN, IDRIS ABU
BIOTROPIA Vol. 13 No. 2 (2006): BIOTROPIA Vol. 13 No. 2 December 2006
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.621 KB) | DOI: 10.11598/btb.2006.13.2.221

Abstract

Schizophyllum commune Fr., is one of the important fungi, causes brown germ and seed rot of oil palm. Biodiversity of antagonistic bacteria from oil palm plantations in Peninsular Malaysia is expected to support in development of biopesticide. Isolation with liquid assay and screening antagonistic bacteria using dual culture assay were carried out in the bioexploration. A total of 265 bacterial isolates from plant parts of oil palm screened 52 antagonistic bacterial isolates against 5. commune. Bacterial isolates were identified by using Biolog* Identification System i.e. Bacillus macroccanus, B. thermoglucosidasius, Burkholderia cepacia, B. gladioli, B. multivorans, B pyrrocinia, B. spinosa, Corynebacterium agropyri, C. misitidis, Enterobacter aerogenes, Microbacterium testaceum, Pseudomonas aeruginosa, P. citronellolis, Rhodococcus rhodochrous, Serratia ficaria, Serratia sp., S. marcescens, Staphylococcus sciuri, Sternotrophomonas maltophilia.Key words : Schizophyllum commune, biodiversity, antagonistic bacteria
Co-Authors Dikin, Antario DIKIN, ANTARJO Inon Sulaiman KADIR, JUGAH Nurhadi . Seman, Idris Abu