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Uji Parameter Non Spesifik dan Aktivitas Antibakteri Ekstrak Metanol dari Umbi Tanaman Dahlia (Dahlia variabilis) Haryani, Yuli; Muthmainah, Siti; Sikumbang, Saryono
Jurnal Penelitian Farmasi Indonesia Vol 1, No 2 (2013)
Publisher : Jurnal Penelitian Farmasi Indonesia

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Abstract

Dahlia variabilis is one of plant that commonly growing in highland hills in Indonesia. The preliminary screening on secondary metabolitesshowed that the presence of flavonoids, phenolics and saponin in methanol extract of this plant’s tuber. Its methanol extract was subjectedto the evaluation of non-specific parameter of extract standardization as well as antimicrobial assay using disc diffusion method againstEscherichia coli and Staphylococcus aureus. The result revealed that the methanol extract contained 25.48±1.63% of water content,4.06±0.15% of ash content, and there was no heavy metal contamination detected. Antibacterial activity assay of three differenceconcentrations of the extract (10; 8 and 2%) demonstrated that extract with 10% of concentration gave the strongest activity against twotested bacterium with diameter of inhibition of 8.67±0.57 and 8.33±0.28 mm against Escherichia coli and Staphylococcus aureus, respectively.
PENGARUH SUHU TERHADAP PRODUKSI ASAM LEVULINAT DARI INULIN UMBI DAHLIA (Dahlia Sp.) Murwindra, Rosa; Sikumbang, Saryono; Awaliddin, Amir; Linggawati, Amilia
Sistem Informasi Vol 7 No 01 (2016): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Dahlia is a floriculture plant with tuber that rich in inulin. Inulin is soluble in hot water and it will settle on cool temperature in alcohol. The inulin extraction to use hot water from dahlia tuber take from Bukittinggi of West Sumatra. Extraction result from 100 g dahlia tuber is 4% inulin (w/w dahlia tuber), such as inulin flour is white gray. As qualitative test with Seliwanoff and Nelson-Somogyi methods, that inulin positive contain of fructose and reducing sugar. This inulin can be converted into levulinic acid using acid catalyst at high temperature. Production of levulinic acid with variate reaction temperature from 130 – 170 oC. The reaction of inulin convertion is done in ampul that burned in oven. In this experiment, conversion of inulin to be levulinic acid is high 26,25% (w/w inulin) using acid catalyst of hydrochloride acid 1 M and heating 170 oC about 15 minutes.