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Sisunandar Sisunandar
Biology Education Department, University of Muhammadiyah Purwokerto Jl. Raya Dukuhwaluh, Kembaran, Purwokerto, 53182, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Health Professions Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Other

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Journal : Proceeding Biology Education Conference

 1 
KEMAJUAN PENELITIAN INDUKSI DAN PEMANJANGAN TUNAS MERBAU (INTSIA BIJUGA [COLEBR.] O. KUNTZE) SECARA IN VITRO Ramadhani, Latifah Esti; Husin, Arief; Sisunandar, Sisunandar
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Abstract - Merbau is one of the high-quality timber tree with the demanding of international market increase every year. However, the over-exploitation of the tree put merbau as a vulnerable species in the future years. Moreover, seedling production of merbau using traditional technique showed several limitations  due to the low number of seed production every year. One of the most promising technique for mass seedling production of merbau is using shoot-tip culture. However, the protocol of shoot-tip culture is remain under develop with low success rate of shoot induction (less then 15 %), without protocols on shoot multiplication, root induction and acclimatisation. Our research at the Laboratory of Botany and Genetics, University of Muhammadiyah Purwokerto showed that the axillary buds isolated from 1-month seedlings can be maintained to induce shoot formation and elongation on the DKW medium (Driver and Kuniyuki, 1984) supplemented with 10-8 M kinetin. Two new shoots started to elongate from each explant after 4 weeks culture (60 % success rate) with each shoot has the lenght of c.a. 4 cm and 4 internode. The inducted shoots were then cut them of into four new axilary buds and subcultured onto the same medium every two week for shoots multiplication. The next step of shoot-tip culture technique, root induction and acclimatization stages is remain developed. Keywords : kinetin, shoot-tip culture,multiplication,micropropagation.
PRODUKSI BIBIT KELAPA KOPYOR TRUE-TO-TYPE MELALUI TEKNIK KULTUR EMBRYO Sisunandar, Sisunandar
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Kopyor is one of the most valuable germplasm in Indonesia. However, the production of true-to-type seedlings with 100 % kopyor nuts is still limited because the nut would not germinated naturally. The only technique available to solve the problem is by using embryo culture technique. However, the technique is limited especially on the low of success rate after acclimatisation step (less than 40 %). Therefore, we report a new breakthough technique for producing kopyor seedling using embryo culture technique especially on acclimatisation step. Our results showed that the best acclimatisation step could be done by culturing 4-month old seedlings in a culture room with 14 hour light with intensity around 1400 lux and 10 hours in the dark. Seedlings were cultured for three months in mini growth chamber with 95 % relative humidity level and temperature around 26 to 28 0C. The survival rate after acclimatisation step on the seedlings with full root was more than 95 %, while for the seedlings without root was c.a 90 %. The acclimatized seedlings then underwent to screenhouse for 1 year befor being field planting. Up to now, more than 170 seedlings of Kopyor have been transfer to the field for developing seed garden of Kopyor in Purwokerto, Indonesia. In the future, creating a breeding program to produce hybrid of Kopyor will be useful for poverty reduction programmes in Indonesia. Keywords: acclimatization, growth chamber, hybrid of kopyor, seed genebank
Co-Authors Arief Husin, Arief Latifah Esti Ramadhani Latifah, Nisa Khoeri