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All Journal Indonesian Journal of Agricultural Science BERITA BIOLOGI
Sobrizal Sobrizal
Center for the Application of Isotopes and Radiation Technology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya, P.O.Box 7002 JKSL, Jakarta 12070, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Journal : BERITA BIOLOGI

 1 
INDUKSI KALUS DAN REGENERASI TANAMAN DARI KULTUR ANTERA PADI Fl HYBRID (ORYZA SATIVA L.) Dwimahyani, Ita; Ishak, Ishak; Sobrizal, Sobrizal
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.767 KB) | DOI: 10.14203/beritabiologi.v5i1.1094

Abstract

A haploid breeding program was initiated to develop doubled haploid blast tolerance rice breeding via anther culture.Blast tolerant variety (Laka) was crossed with Kencana Bali (sensitive to blast).Anthers from F1 were cultured on two kind of media (combination of N6 macro salt and MS micro salt) containing 1 mg/l NAA + 0,1 mg/l kinetin called medium-1 and another one containing 1 mg/l 2,4-D + 0,1 mg/l kinetin called medium-2.Anthers were treated with cold shock (4 °C)for 5 and 10 days before cultured.Results of experiment showed that F1 plants derived anthers were able to form call Number ofplantlets were produced during anthers culture consisted of 482 green plants and 50 albinos. Most of green plants did not produced root.Root growth of plantlets were induced with 1.5-4.5 mg/l IBA in MS medium.
Co-Authors Ahmad Warsun Atsushi Yoshimura Carkum Carkum Ishak Ishak Ita Dwimahyani Masdiar Bustamam Soeranto Human Yoshimichi Fukuta