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IDENTIFICATION OF A MAJOR QUANTITATIVE TRAIT LOCUS CONFERRING RICE BLAST RESISTANCE USING RECOMBINANT INBRED LINES Sobrizal, Sobrizal; Bustamam, Masdiar; Carkum, Carkum; Warsun, Ahmad; Human, Soeranto; Fukuta, Yoshimichi
Indonesian Journal of Agricultural Science Vol 11, No 1 (2010): April 2010
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Blast disease caused by Pyricularia oryzae is one of the limitingfactors for rice production world wide. The use of resistantvarieties for managing blast disease is considered as the mosteco-friendly approaches. However, their resistances may bebroken down within a few years due to the appearance of newvirulent blast races in the field. The objective of the presentstudy was to identify the quantitative trait locus (QTL) conferringresistance to blast disease using 126 recombinant inbred(RI) lines originated from a crossing of a durably resistant uplandrice genotype (Laka) and a highly susceptible rice accessioncultivar (Kencana Bali). The RI population was developedthrough a single seed descent method from 1997 to 2004.Resistance of the RI lines was evaluated for blast in an endemicarea of Sukabumi, West Java, in 2005. Disease intensity of theblast was examined following the standard evaluation systemdeveloped by the International Rice Research Institute (IRRI).At the same year the RI lines were analyzed with 134 DNAmarkers. Results of the study showed that one major QTL wasfound to be associated with blast resistance, and this QTL waslocated near RM2136 marker on the long arm of chromosome11. This QTL explained 87% of the phenotypic variation with37% additive effect. The map position of this QTL differedfrom that of a partial resistant gene, Pi34, identified previouslyon chromosome 11 in the Japanese durably resistant variety,Chubu 32. The QTL, however, was almost at the same positionas that of the multiple allele-resistant gene, Pik. Therefore, anallelic test should be conducted to clarify the allelic relationshipbetween QTL identified in this study and the Pik. The RI linesare the permanent segregating population that could be veryuseful for analysing phenotypic variations of important agronomictraits possibly owned by the RI lines. The major QTLidentified in this study could be used as a genetic resource inimprovement of rice varieties for blast resistance in Indonesia
Determination of seed physiological maturity and storability of several sorghum varieties Faizal, Karima Rizki; Qadir, Abdul; Human, Soeranto; Ilyas, Satriyas
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.63421

Abstract

Seed vigor reaches a maximum at physiological maturity and decreases during storage. This study aimed to determine the physiological maturity and enhance the storability of sorghum seeds. Experiments were conducted at BRIN Lebak-Bulus from March 2023 to March 2024. The initial experiment employed a two-factor randomized complete block design, incorporating five sorghum varieties and five seed maturity levels. The second experiment used a complete randomized design with two factors (nest: temperature storage 18 °C and 26 °C, and nested: combined seed moisture content (MC) 10-11% and 12-13%, and storage period (1-24 weeks). The results indicated the seed physiological maturity stages of the five sorghum varieties were: Numbu 46 days after anthesis (DAA), Super-1 39 DAA, Bioguma-1 51 DAA, Samurai-2 55 DAA, and Pahat 40 DAA. The Numbu stored at 18 °C, 10-11% MC had storability up to 24 weeks (72.3% germination) and at 18 °C, 12-13% MC up to 20 weeks (72.3%), while at 26 °C, 10-11% MC had storability up to 18 weeks (71.3%) and 26 °C, 12-13% MC up to 22 weeks (70.3%). The Bioguma-1 and Samurai-2 seeds were suspected of having after-ripening dormancy. From the beginning until 24 weeks, they did not deteriorate, although the viability was below 70%. Keywords: germination; seed moisture content; storage period; viability; vigor
Pendugaan Parameter Genetik dan Seleksi Galur Mutan Sorgum (Sorghum bicolor (L.) Moench) di Tanah Masam (Estimation of Genetic Parameters and Selection of Sorghum Mutant Lines under Acid Soil Stress Conditions) Sungkono, Sungkono; Trikoesoemaningtyas, Trikoesoemaningtyas; Wirnas, Desta; Sopandie, Didy; Human, Soeranto; Yudiarto, Muhammad Arif
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.958 KB) | DOI: 10.24831/jai.v37i3.1238

Abstract

  Sorghum is one of high value commodities for food and energy security due to its wide adaptation. The objectives of this research were to study some genetic parameters of sorghum agronomic traits grown under acid soil conditions and to select aluminum-tolerant sorghum mutant lines.  The selection was conducted in an augmented design with 61 lines at the field research station of B2TP-BPPT Lampung. Genetic materials used were mutant lines developed throught gamma irradiation.  The results showed that there was significant differences among the mutant lines for  some agronomic characters observed.  The heritability estimates for agronomic characters were classified as intermediate to high.  Selection based on seed weight and biomass production resulted three best sorghum mutan lines i.e ZH30-29-07, ZH30-30-07, and ZH30-35-07.  These lines showed the best yield and biomass production under acid soil condition.   Key words:  sorghum, acid soil, heritability, selection