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Lestari Mahardika Urbaningrum
Universitas Singaperbangsa Karawang (UNSIKA) Jawa Barat, Indonesia
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Isolasi dan Identifikasi Senyawa Flavonoid pada Tanaman Audi Ichsani; Christina Febiola Lubis; Lestari Mahardika Urbaningrum; Nurma Dwi Rahmawati; Sridevi Anggraini
Jurnal Health Sains Vol. 2 No. 6 (2021): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v2i6.188

Abstract

Isolation of flavonoid compounds can be done in various stages. In this study, a literature study was done to determine the procedure for the Isolation and Identification of Flavonoid Compounds. The procedure usually begins with a phytochemical test (screening) first. Flavonoid phytochemical tests can be done with various types of tests, Bate Smith-Matcalfe test, 10% NaOH test, and Wilstatter test. It can be done with all of the tests or just one of them. The first step is extraction with a suitable solvent. The suitable solvents for the isolation of flavonoid compounds are polar and semi-polar solvents. Extraction of plant compounds for isolated flavonoid compounds used the maceration method. Maceration carried out for 72 hours while stirring every 24 hours. After that the extract was concentrated with a rotary vacuum evaporator. The next step is fractionation. For the fractionation of isolated flavonoid compounds, it can be done by the methods of Liquid-Liquid Extraction, Vacuum Liquid Chromatography, Column Chromatography, and Thin Layer Chromatography. Solvents that can be used in this step vary, depending on the method. Then, isolate be monitored to determine the type of flavonoid contained in the isolate. The methods that can be used in this step are UV-Vis Spectrophotometer, HNMR, and FTIR spectrophotometer. You can use one method or two methods.
Metode Analisis Kualitatif Senyawa Obat Methamphetamine pada Sampel Rambut Bunga Nur Annisa; Lestari Mahardika Urbaningrum; Nena Vauziah Sary; Cantika Nurul Sa’adah; Audi Ichsani Aribowo; Christina Febiola Lubis
Jurnal Health Sains Vol. 3 No. 4 (2022): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v3i4.478

Abstract

Methamphetamine (C10H15N) is a class two psychotropic, a stimulant that acts on the central nervous system, causing addiction when consumed. Hair as a part of the body is often used as an option to detect the presence of methamphetamine in the body. The advantage of hair is that the presence of a drug can last weeks to months. This review was done to see how methamphetamine was detected in hair samples. As a result, it is known that the detection of methamphetamine in hair samples can be carried out using the Solid Phase Extraction (SPE) method. The procedure begins with the preparation of Marquish reagent by mixing 37% formaldehyde with glacial acetic acid. The procedure continued with the preparation of hair samples by grinded 30-40 mg of hair and then washed it with ethanol. After that the sample was sonicated with various solvent systems at room temperature for 30 minutes. The last step is column extraction conducted by inserting the filtrate into the column basement membrane, the column was conditioned, then the sonicated analyte was added and carried out with dichloromethane: 4 ml, then eluted and the sample eluent obtained was identified using marquish reagent
Co-Authors Audi Ichsani Audi Ichsani Aribowo Bunga Nur Annisa Cantika Nurul Sa’adah Christina Febiola Lubis Nena Vauziah Sary Nurma Dwi Rahmawati Sridevi Anggraini